Recombinant Human RPS20 protein is a Human Full Length protein, in the 1 to 142 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A F K D T G K T P V E P E V A I H R I R I T L T S R N V K S L E K V C A D L I R G A K E K N L K V K G P V R M P T K T L R I T T R K T P C G E G S K T W D R F Q M R I H K R L I D L H S P S E I V K Q I T S I S I E P G V E L I E S T D A E P M D T E G Q Q Y T L R S V F E S P G T C P F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the small ribosomal subunit (PubMed:23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399).
Alternative names
Small ribosomal subunit protein uS10, 40S ribosomal protein S20, RPS20
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Recombinant Human RPS20 protein is a Human Full Length protein, in the 1 to 142 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P60866-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% EDTA, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A F K D T G K T P V E P E V A I H R I R I T L T S R N V K S L E K V C A D L I R G A K E K N L K V K G P V R M P T K T L R I T T R K T P C G E G S K T W D R F Q M R I H K R L I D L H S P S E I V K Q I T S I S I E P G V E L I E S T D A E P M D T E G Q Q Y T L R S V F E S P G T C P F
- Accession
- P60866
- Protein length
- Full Length
- Predicted molecular weight
- 18.4 kDa
- Amino acids
- 1 to 142
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab174426 is purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
General info
- Function
Component of the small ribosomal subunit (PubMed:23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399).
- Sequence similarities
Belongs to the universal ribosomal protein uS10 family.
- Post-translational modifications
Polyubiquitinated by ZNF598 via 'Lys-63'-linked ubiquitin chains when a ribosome has stalled, initiating the ribosome quality control (RQC) pathway to degrade the potentially detrimental aberrant nascent polypeptide (PubMed:28065601, PubMed:28132843, PubMed:28685749, PubMed:32011234, PubMed:36302773). Deubiquitinated by OTUD3 and USP21, antagonizing ZNF598 activity (PubMed:32011234).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RPS20 also known as Ribosomal Protein S20 is a component of the ribosome with a molecular weight of approximately 13 kDa. It operates within the small 40S subunit of the ribosome and plays an important role in protein synthesis by facilitating translation. RPS20 shows expression mainly in the cytosol and nucleus of cells where ribosome assembly and function occur.
- Biological function summary
This ribosomal protein contributes to accurate translation and assists in the binding of tRNA and mRNA to the ribosome. RPS20 is an essential part of the ribosomal complex that influences the fidelity and efficiency of protein synthesis. Any alterations in ribosomal protein components like RPS20 can lead to downstream effects on protein production impacting cellular processes such as growth and differentiation.
- Pathways
The activity of RPS20 is associated with the ribosome biogenesis and translation pathways which are central to cellular protein synthesis. It closely interacts with other ribosomal proteins such as RPS19 and RPS21 within these pathways to ensure proper ribosome assembly and function. This interplay maintains the balance of protein synthesis influencing cellular metabolism and overall homeostasis.
- Associated diseases and disorders
Mutations or dysregulation in RPS20 are linked to certain forms of ribosomopathies such as Diamond-Blackfan anemia. This disorder is characterized by impaired red blood cell production where ribosomal protein mutations disrupt normal erythropoiesis. RPS20's connection to ribosomopathies also relates to other ribosomal proteins like RPL11 which share involvement in similar pathways and disorders.
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SDS-PAGE - Recombinant Human RPS20 protein (ab174426)
SDS-PAGE analysis of ab174426 on 15% SDS-PAGE gel (3μg).
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