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AB137146

Recombinant Human RPS5 protein

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(2 Publications)

Recombinant Human RPS5 protein is a Human Full Length protein, in the 1 to 204 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Small ribosomal subunit protein uS7, 40S ribosomal protein S5, RPS5

1 Images
SDS-PAGE - Recombinant Human RPS5 protein (AB137146)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RPS5 protein (AB137146)

15% SDS-PAGE analysis of 3 µg ab137146.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P46782

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMTEWETAAPAVAETPDIKLFGKWSTDDVQINDISLQDYIAVKEKYAKYLPHSAGRYAAKRFRKAQCPIVERLTNSMMMHGRNNGKKLMTVRIVKHAFEIIHLLTGENPLQVLVNAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR","proteinLength":"Full Length","predictedMolecularWeight":"25.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":204,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P46782","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS5 also known as 40S ribosomal protein S5 plays an essential role in the structure and function of ribosomes. It is a component of the 40S subunit of ribosomes weighing approximately 21.8 kDa. RPS5 is expressed ubiquitously in a variety of cellular compartments including the cytoplasm where ribosomes are actively involved in protein synthesis. This protein is part of the S7P family of ribosomal proteins and contributes to the assembly and stability of the ribosomal structure.
Biological function summary

The protein forms part of the small ribosomal subunit and contributes to the translation of mRNA into proteins. It cooperates with other ribosomal proteins and RNA to ensure the accurate decoding of mRNA sequences during protein synthesis. RPS5 interacts with multiple components of the ribosome to maintain the efficiency and fidelity of translation. It is important for the maturation and assembly of the ribosomal machinery which is essential in cell growth and division.

Pathways

The translation process heavily depends on the activity of RPS5. It participates in the ribosome biogenesis pathway impacting translational control and protein homeostasis. It interacts with RPL5 and several other ribosomal proteins which are important for the proper functioning of the translation process. RPS5 also plays a role in the mTOR signaling pathway which influences cellular growth and metabolism by responding to nutrient and energy status.

Mutations or dysregulation of RPS5 relate to cancer and Diamond-Blackfan anemia. In certain cancers changes in ribosomal proteins like RPS5 disrupt normal protein synthesis contributing to unchecked cell proliferation. In Diamond-Blackfan anemia insufficient production of ribosomal proteins including RPS5 leads to impaired red blood cell production. RPS5 in conjunction with other ribosomal proteins becomes a focal point to study these diseases for potential therapeutic targets.
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Specifications

Form

Liquid

Additional notes

ab137146 is purified using conventional chromatography techniques.

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General info

Function

Component of the small ribosomal subunit (PubMed : 23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

Sequence similarities

Belongs to the universal ribosomal protein uS7 family.

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Product protocols

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Target data

Component of the small ribosomal subunit (PubMed : 23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information 40S ribosomal protein S5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 5:564 PubMed35681048

2022

Stabilization of CDK6 by ribosomal protein uS7, a target protein of the natural product fucoxanthinol.

Applications

Unspecified application

Species

Unspecified reactive species

Yosuke Iizumi,Yoshihiro Sowa,Wakana Goi,Yuichi Aono,Motoki Watanabe,Yoichi Kurumida,Tomoshi Kameda,Kenichi Akaji,Masatoshi Kitagawa,Toshiyuki Sakai

PNAS nexus 1:pgac059 PubMed36713317

2022

A chemoproteoinformatics approach demonstrates that aspirin increases sensitivity to MEK inhibition by directly binding to RPS5.

Applications

Unspecified application

Species

Unspecified reactive species

Motoki Watanabe,Shogen Boku,Kaito Kobayashi,Yoichi Kurumida,Mamiko Sukeno,Mitsuharu Masuda,Katsura Mizushima,Chikage Kato,Yosuke Iizumi,Kiichi Hirota,Yuji Naito,Michihiro Mutoh,Tomoshi Kameda,Toshiyuki Sakai
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Complementary Products

Primary Antibodies

AB32158

Anti-Bim antibody [Y36]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Bim antibody [Y36] (AB32158)

  • 4
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Primary Antibodies

AB210745

Anti-RPS5 antibody

Western blot - Anti-RPS5 antibody (AB210745)

  • 0
  • 0
Primary Antibodies

AB241412

Anti-RPL3 antibody

Immunoprecipitation - Anti-RPL3 antibody (AB241412)

  • 0
  • 0
Primary Antibodies

AB74711

Anti-RPS9 antibody

Western blot - Anti-RPS9 antibody (AB74711)

  • 0
  • 0

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