Recombinant Human RPS5 protein is a Human Full Length protein, in the 1 to 204 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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- Communications biology 5:5642022Stabilization of CDK6 by ribosomal protein uS7, a target protein of the natural product fucoxanthinol.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYosuke Iizumi,Yoshihiro Sowa,Wakana Goi,Yuichi Aono,Motoki Watanabe,Yoichi Kurumida,Tomoshi Kameda,Kenichi Akaji,Masatoshi Kitagawa,Toshiyuki SakaiPubMed 35681048
- PNAS nexus 1:pgac0592022A chemoproteoinformatics approach demonstrates that aspirin increases sensitivity to MEK inhibition by directly binding to RPS5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMotoki Watanabe,Shogen Boku,Kaito Kobayashi,Yoichi Kurumida,Mamiko Sukeno,Mitsuharu Masuda,Katsura Mizushima,Chikage Kato,Yosuke Iizumi,Kiichi Hirota,Yuji Naito,Michihiro Mutoh,Tomoshi Kameda,Toshiyuki SakaiPubMed 36713317
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M T E W E T A A P A V A E T P D I K L F G K W S T D D V Q I N D I S L Q D Y I A V K E K Y A K Y L P H S A G R Y A A K R F R K A Q C P I V E R L T N S M M M H G R N N G K K L M T V R I V K H A F E I I H L L T G E N P L Q V L V N A I I N S G P R E D S T R I G R A G T V R R Q A V D V S P L R R V N Q A I W L L C T G A R E A A F R N I K T I A E C L A D E L I N A A K G S S N S Y A I K K K D E L E R V A K S N R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the small ribosomal subunit (PubMed:23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
Alternative names
Small ribosomal subunit protein uS7, 40S ribosomal protein S5, RPS5
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Recombinant Human RPS5 protein is a Human Full Length protein, in the 1 to 204 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P46782-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M T E W E T A A P A V A E T P D I K L F G K W S T D D V Q I N D I S L Q D Y I A V K E K Y A K Y L P H S A G R Y A A K R F R K A Q C P I V E R L T N S M M M H G R N N G K K L M T V R I V K H A F E I I H L L T G E N P L Q V L V N A I I N S G P R E D S T R I G R A G T V R R Q A V D V S P L R R V N Q A I W L L C T G A R E A A F R N I K T I A E C L A D E L I N A A K G S S N S Y A I K K K D E L E R V A K S N R
- Accession
- P46782
- Protein length
- Full Length
- Predicted molecular weight
- 25.3 kDa
- Amino acids
- 1 to 204
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab137146 is purified using conventional chromatography techniques.
General info
- Function
Component of the small ribosomal subunit (PubMed:23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
- Sequence similarities
Belongs to the universal ribosomal protein uS7 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RPS5 also known as 40S ribosomal protein S5 plays an essential role in the structure and function of ribosomes. It is a component of the 40S subunit of ribosomes weighing approximately 21.8 kDa. RPS5 is expressed ubiquitously in a variety of cellular compartments including the cytoplasm where ribosomes are actively involved in protein synthesis. This protein is part of the S7P family of ribosomal proteins and contributes to the assembly and stability of the ribosomal structure.
- Biological function summary
The protein forms part of the small ribosomal subunit and contributes to the translation of mRNA into proteins. It cooperates with other ribosomal proteins and RNA to ensure the accurate decoding of mRNA sequences during protein synthesis. RPS5 interacts with multiple components of the ribosome to maintain the efficiency and fidelity of translation. It is important for the maturation and assembly of the ribosomal machinery which is essential in cell growth and division.
- Pathways
The translation process heavily depends on the activity of RPS5. It participates in the ribosome biogenesis pathway impacting translational control and protein homeostasis. It interacts with RPL5 and several other ribosomal proteins which are important for the proper functioning of the translation process. RPS5 also plays a role in the mTOR signaling pathway which influences cellular growth and metabolism by responding to nutrient and energy status.
- Associated diseases and disorders
Mutations or dysregulation of RPS5 relate to cancer and Diamond-Blackfan anemia. In certain cancers changes in ribosomal proteins like RPS5 disrupt normal protein synthesis contributing to unchecked cell proliferation. In Diamond-Blackfan anemia insufficient production of ribosomal proteins including RPS5 leads to impaired red blood cell production. RPS5 in conjunction with other ribosomal proteins becomes a focal point to study these diseases for potential therapeutic targets.
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SDS-PAGE - Recombinant Human RPS5 protein (ab137146)
15% SDS-PAGE analysis of 3 µg ab137146.
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