Recombinant Human RQCD1 protein is a Human Full Length protein, in the 1 to 299 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M H S L A T A A P V P T T L A Q V D R E K I Y Q W I N E L S S P E T R E N A L L E L S K K R E S V P D L A P M L W H S F G T I A A L L Q E I V N I Y P S I N P P T L T A H Q S N R V C N A L A L L Q C V A S H P E T R S A F L A A H I P L F L Y P F L H T V S K T R P F E Y L R L T S L G V I G A L V K T D E Q E V I N F L L T T E I I P L C L R I M E S G S E L S K T V A T F I L Q K I L L D D T G L A Y I C Q T Y E R F S H V A M I L G K M V L Q L S K E P S A R L L K H V V R C Y L R L S D N P R A R E A L R Q C L P D Q L K D T T F A Q V L K D D T T T K R W L A Q L V K N L Q E G Q V T D P R G I P L P P Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in down-regulation of MYB- and JUN-dependent transcription. May play a role in cell differentiation (By similarity). Can bind oligonucleotides, such as poly-G, poly-C or poly-T (in vitro), but the physiological relevance of this is not certain. Does not bind poly-A. Enhances ligand-dependent transcriptional activity of nuclear hormone receptors, including RARA, expect ESR1-mediated transcription that is not only slightly increased, if at all.
Alternative names
CCR4-NOT transcription complex subunit 9, Cell differentiation protein RQCD1 homolog, Rcd-1, RCD1, RQCD1, CNOT9
Recommended products
Recombinant Human RQCD1 protein is a Human Full Length protein, in the 1 to 299 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Alternative names
CCR4-NOT transcription complex subunit 9, Cell differentiation protein RQCD1 homolog, Rcd-1, RCD1, RQCD1, CNOT9
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Accession
- Q92600-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M H S L A T A A P V P T T L A Q V D R E K I Y Q W I N E L S S P E T R E N A L L E L S K K R E S V P D L A P M L W H S F G T I A A L L Q E I V N I Y P S I N P P T L T A H Q S N R V C N A L A L L Q C V A S H P E T R S A F L A A H I P L F L Y P F L H T V S K T R P F E Y L R L T S L G V I G A L V K T D E Q E V I N F L L T T E I I P L C L R I M E S G S E L S K T V A T F I L Q K I L L D D T G L A Y I C Q T Y E R F S H V A M I L G K M V L Q L S K E P S A R L L K H V V R C Y L R L S D N P R A R E A L R Q C L P D Q L K D T T F A Q V L K D D T T T K R W L A Q L V K N L Q E G Q V T D P R G I P L P P Q
- Accession
- Protein length
Full Length
- Predicted molecular weight
35.7 kDa
- Amino acids
1 to 299
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
ab101208 was purified using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
General info
- Function
Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in down-regulation of MYB- and JUN-dependent transcription. May play a role in cell differentiation (By similarity). Can bind oligonucleotides, such as poly-G, poly-C or poly-T (in vitro), but the physiological relevance of this is not certain. Does not bind poly-A. Enhances ligand-dependent transcriptional activity of nuclear hormone receptors, including RARA, expect ESR1-mediated transcription that is not only slightly increased, if at all.
- Sequence similarities
Belongs to the CNOT9 family.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RQCD1 also known as Required for Cell Differentiation 1 is a protein with an approximate mass of 34 kDa. It plays an essential role in gene expression regulation by acting as a transcriptional cofactor. RQCD1 is highly expressed in various tissues including the liver kidney and heart. The protein interacts with different transcription factors and modulates their activities which influences cellular pathways critical for cell development and differentiation.
- Biological function summary
The protein RQCD1 acts as a component of the human mRNA methyltransferase-associated complex. The complex is involved in post-transcriptional modifications. Through its interaction in the complex RQCD1 contributes to RNA processing and stability further affecting gene expression outcomes. This involvement impacts cell cycle regulation and proliferation necessary for maintaining cellular homeostasis.
- Pathways
The protein RQCD1 is important in the MAPK signaling pathway and Wnt signaling pathway. In these pathways it interacts with proteins like c-Fos and β-catenin influencing pathways related to cell growth and differentiation. Its presence in these pathways demonstrates the protein's role in signal transduction processes that govern various cellular responses.
- Associated diseases and disorders
Proteins like RQCD1 have shown associations with cancer and metabolic disorders. Aberrant expression or mutations in RQCD1 are linked to the development and progression of certain cancers due to its role in transcriptional regulation. Additionally this protein has connections with metabolic proteins like AMPK indicating its potential impact on disorders related to metabolic imbalance. These roles suggest that RQCD1 is a significant player in both cancer biology and metabolism-related diseases.
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1 product image
SDS-PAGE - Recombinant Human RQCD1 protein (ab101208)
ab101208 at 3 μg analysed by 15% SDS PAGE.
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