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AB160946

Recombinant Human RRAGB protein

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(1 Publication)

Recombinant Human RRAGB protein is a Human Full Length protein, in the 1 to 346 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

Ras-related GTP-binding protein B, Rag B, RagB, RRAGB

1 Images
SDS-PAGE - Recombinant Human RRAGB protein (AB160946)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RRAGB protein (AB160946)

ab160946 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q5VZM2

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MEESDSEKTTEKENLGPRMDPPLGEPEGSLGWVLPNTAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATIDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPDAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECSCFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLRNFAEIIEADEVLLFERATFLVISHYQCKEQRDAHRFEKISNIIKQFKLSCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKLERVDGPKQCLLMR","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":346,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q5VZM2","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RRAGB also known as Ras-related GTPase B is a member of the Rag family of GTPases. This protein has a molecular mass of approximately 42 kDa. RRAGB is expressed in various tissues with significant expression in the brain and skeletal muscles. The protein functions as a molecular switch toggling between active GTP-bound and inactive GDP-bound states. This cycling allows RRAGB to regulate mTORC1 signaling which is essential for cellular growth and metabolism.
Biological function summary

RRAGB partners with other Rag GTPases to form heterodimeric complexes. These complexes facilitate the proper localization and activation of mTORC1 on the lysosomal membrane. Moreover RRAGB plays a role in amino acid sensing which impacts cellular growth. By responding to intracellular nutrient levels RRAGB helps modulate anabolic processes and energy balance.

Pathways

RRAGB is integral to the mTOR signaling and nutrient-sensing pathways. As a part of these pathways RRAGB collaborates with proteins such as Raptor and Rheb. These interactions ensure efficient transmission of signals that regulate cell growth and metabolism. The accurate function of this network supports cellular adaptation to varying environmental nutrient conditions.

RRAGB has been associated with certain cancers and neurodegenerative diseases. Alterations in the mTOR pathway involving RRAGB can contribute to undesired cellular proliferation observed in cancers. Moreover disruptions in RRAGB activity can influence mTORC1 signaling in neurodegenerative conditions. It's important to further understand how RRAGB and related proteins like mTOR are involved in the pathology of these diseases to develop targeted therapies.
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Specifications

Form

Liquid

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General info

Function

Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade (PubMed : 18497260, PubMed : 20381137, PubMed : 23723238, PubMed : 24095279). Forms heterodimeric Rag complexes with RagC/RRAGC or RagD/RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form : RagB/RRAGB is in its active form when GTP-bound RagB/RRAGB forms a complex with GDP-bound RagC/RRAGC (or RagD/RRAGD) and in an inactive form when GDP-bound RagB/RRAGB heterodimerizes with GTP-bound RagC/RRAGC (or RagD/RRAGD) (PubMed : 18497260, PubMed : 20381137, PubMed : 23723238, PubMed : 24095279). In its GTP-bound active form, promotes the recruitment of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB (PubMed : 18497260, PubMed : 20381137, PubMed : 23723238). Involved in the RCC1/Ran-GTPase pathway (PubMed : 9394008).

Sequence similarities

Belongs to the GTR/RAG GTP-binding protein family.

Subcellular localisation

Lysosome

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Product protocols

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Target data

Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade (PubMed : 18497260, PubMed : 20381137, PubMed : 23723238, PubMed : 24095279). Forms heterodimeric Rag complexes with RagC/RRAGC or RagD/RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form : RagB/RRAGB is in its active form when GTP-bound RagB/RRAGB forms a complex with GDP-bound RagC/RRAGC (or RagD/RRAGD) and in an inactive form when GDP-bound RagB/RRAGB heterodimerizes with GTP-bound RagC/RRAGC (or RagD/RRAGD) (PubMed : 18497260, PubMed : 20381137, PubMed : 23723238, PubMed : 24095279). In its GTP-bound active form, promotes the recruitment of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB (PubMed : 18497260, PubMed : 20381137, PubMed : 23723238). Involved in the RCC1/Ran-GTPase pathway (PubMed : 9394008).
See full target information RRAGB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Regenerative therapy 26:458-468 PubMed39100534

2024

Berberine ameliorates endothelial progenitor cell function and wound healing and via the miR-21-3p/RRAGB axis for venous leg ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Qiao,Lingying Niu,Weihua Jiang,Li Lu,Jiali Liu
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