Recombinant Human RRM1 protein is a Human Fragment protein, in the 593 to 792 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
I R N S L L I A P M P T A S T A Q I L G N N E S I E P Y T S N I Y T R R V L S G E F Q I V N P H L L K D L T E R G L W H E E M K N Q I I A C N G S I Q S I P E I P D D L K Q L Y K T V W E I S Q K T V L K M A A E R G A F I D Q S Q S L N I H I A E P N Y G K L T S M H F Y G W K Q G L K T G M Y Y L R T R P A A N P I Q F T L N K E K L K D K E K V S K E E E E K E R N T A A M V C S L E N R D E C L M C G S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
Alternative names
RR1, RRM1, Ribonucleoside-diphosphate reductase large subunit, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleotide reductase large subunit
Recommended products
Recombinant Human RRM1 protein is a Human Fragment protein, in the 593 to 792 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P23921-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- I R N S L L I A P M P T A S T A Q I L G N N E S I E P Y T S N I Y T R R V L S G E F Q I V N P H L L K D L T E R G L W H E E M K N Q I I A C N G S I Q S I P E I P D D L K Q L Y K T V W E I S Q K T V L K M A A E R G A F I D Q S Q S L N I H I A E P N Y G K L T S M H F Y G W K Q G L K T G M Y Y L R T R P A A N P I Q F T L N K E K L K D K E K V S K E E E E K E R N T A A M V C S L E N R D E C L M C G S
- Accession
- P23921
- Protein length
- Fragment
- Amino acids
- 593 to 792
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
- Sequence similarities
Belongs to the ribonucleoside diphosphate reductase large chain family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RRM1 also known as ribonucleotide reductase large subunit or RNR1 plays an important role in DNA synthesis and repair converting ribonucleotides to deoxyribonucleotides. This enzyme has a mass of approximately 90 kDa. RRM1 is found in multiple tissues exhibiting especially high expression levels in proliferative tissues and tumors. Its activity is tightly regulated through the cell cycle and it is essential during DNA replication and cell division.
- Biological function summary
The large subunit of ribonucleotide reductase contributes to the enzymatic function through binding and activation of smaller subunits including RRM2 and RRM2B forming a complex. This complex is important for maintaining balanced deoxyribonucleotide pools during the S-phase of the cell cycle. Proper RRM1 function ensures the fidelity of DNA synthesis impacting genomic stability and cell viability.
- Pathways
RRM1 acts within the DNA replication and repair pathways. It interacts with the tumor suppressor protein p53 which modulates its activities in response to DNA damage. Through pathways like the p53-dependent checkpoint RRM1 contributes to DNA damage response mechanisms. This interaction with p53 and involvement in the DNA replication pathway highlight its centrality in maintaining genomic integrity.
- Associated diseases and disorders
RRM1 has connections with cancer and chemoresistance. Overexpression of RRM1 often correlates with increased tumor aggressiveness and poor prognosis in several cancers including lung and pancreatic cancers. In the context of chemoresistance RRM1 interplays with the excision repair cross-complementation group 1 protein (ERCC1) impacting the effectiveness of certain chemotherapies. Its role in these conditions makes it a target for cancer diagnosis and treatment strategies.
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1 product image
SDS-PAGE - Recombinant Human RRM1 protein (ab152668)
ab152668 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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