Recombinant human Rsk 2 / MAPKAP Kinase 1b (mutated L608F) protein is a Human Full Length protein, in the 1 to 740 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB, FuncS.
Images
Key facts
- Purity
>95% Densitometry
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, WB, FuncS
- Biologically active
Yes
Amino acid sequence
M P L A Q L A D P W Q K M A V E S P S D S A E N G Q Q I M D E P M G E E E I N P Q T E E V S I K E I A I T H H V K E G H E K A D P S Q F E L L K V L G Q G S F G K V F L V K K I S G S D A R Q L Y A M K V L K K A T L K V R D R V R T K M E R D I L V E V N H P F I V K L H Y A F Q T E G K L Y L I L D F L R G G D L F T R L S K E V M F T E E D V K F Y L A E L A L A L D H L H S L G I I Y R D L K P E N I L L D E E G H I K L T D F G L S K E S I D H E K K A Y S F C G T V E Y M A P E V V N R R G H T Q S A D W W S F G V L M F E M L T G T L P F Q G K D R K E T M T M I L K A K L G M P Q F L S P E A Q S L L R M L F K R N P A N R L G A G P D G V E E I K R H S F F S T I D W N K L Y R R E I H P P F K P A T G R P E D T F Y F D P E F T A K T P K D S P G I P P S A N A H Q L F R G F S F V A I T S D D E S Q A M Q T V G V H S I V Q Q L H R N S I Q F T D G Y E V K E D I G V G S Y S V C K R C I H K A T N M E F A V K I I D K S K R D P T E E I E I L L R Y G Q H P N I I T L K D V Y D D G K Y V Y V V T E L M K G G E L L D K I L R Q K F F S E R E A S A V L F T I T K T V E Y L H A Q G V V H R D L K P S N I L Y V D E S G N P E S I R I C D F G F A K Q L R A E N G L L M T P C Y T A N F V A P E V L K R Q G Y D A A C D I W S L G V L L Y T M L T G Y T P F A N G P D D T P E E I L A R I G S G K F S L S G G Y W N S V S D T A K D L V S K M L H V D P H Q R L T A A L V L R H P W I V H W D Q L P Q Y Q L N R Q D A P H L V K G A M A A T Y S A L N R N Q S P V L E P V G R S T L A Q R R G I K K I T S T A L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes Kinase assay. Specific Activity: 140 nmol/min/mg. |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1 (PubMed:16213824, PubMed:16223362, PubMed:17360704, PubMed:9770464). In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes (PubMed:10436156, PubMed:9770464). In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP (PubMed:16223362). Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity (PubMed:8250835). Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex (PubMed:17360704). In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation (PubMed:18508509, PubMed:18813292). Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway (PubMed:18722121). Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function (PubMed:16213824). Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4) (PubMed:18508509, PubMed:18813292). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression (By similarity). In LPS-stimulated dendritic cells, is involved in TLR4-induced macropinocytosis, and in myeloma cells, acts as effector of FGFR3-mediated transformation signaling, after direct phosphorylation at Tyr-529 by FGFR3 (By similarity). Negatively regulates EGF-induced MAPK1/3 phosphorylation via phosphorylation of SOS1 (By similarity). Phosphorylates SOS1 at 'Ser-1134' and 'Ser-1161' that create YWHAB and YWHAE binding sites and which contribute to the negative regulation of MAPK1/3 phosphorylation (By similarity). Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration (PubMed:26158630). Acts as a regulator of osteoblast differentiation by mediating phosphorylation of ATF4, thereby promoting ATF4 transactivation activity (By similarity).
Alternative names
ISPK1, MAPKAPK1B, RSK2, RPS6KA3, Ribosomal protein S6 kinase alpha-3, S6K-alpha-3, 90 kDa ribosomal protein S6 kinase 3, Insulin-stimulated protein kinase 1, MAP kinase-activated protein kinase 1b, Ribosomal S6 kinase 2, pp90RSK2, p90-RSK 3, p90RSK3, ISPK-1, MAPK-activated protein kinase 1b, MAPKAP kinase 1b, MAPKAPK-1b, RSK-2
Recommended products
Recombinant human Rsk 2 / MAPKAP Kinase 1b (mutated L608F) protein is a Human Full Length protein, in the 1 to 740 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
>95% Densitometry
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, WB, FuncS
- Biologically active
Yes
- Biological activity
Specific Activity, 140 nmol/min/mg.
- Accession
- P51812-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.61% Tris-HCl buffer, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
M P L A Q L A D P W Q K M A V E S P S D S A E N G Q Q I M D E P M G E E E I N P Q T E E V S I K E I A I T H H V K E G H E K A D P S Q F E L L K V L G Q G S F G K V F L V K K I S G S D A R Q L Y A M K V L K K A T L K V R D R V R T K M E R D I L V E V N H P F I V K L H Y A F Q T E G K L Y L I L D F L R G G D L F T R L S K E V M F T E E D V K F Y L A E L A L A L D H L H S L G I I Y R D L K P E N I L L D E E G H I K L T D F G L S K E S I D H E K K A Y S F C G T V E Y M A P E V V N R R G H T Q S A D W W S F G V L M F E M L T G T L P F Q G K D R K E T M T M I L K A K L G M P Q F L S P E A Q S L L R M L F K R N P A N R L G A G P D G V E E I K R H S F F S T I D W N K L Y R R E I H P P F K P A T G R P E D T F Y F D P E F T A K T P K D S P G I P P S A N A H Q L F R G F S F V A I T S D D E S Q A M Q T V G V H S I V Q Q L H R N S I Q F T D G Y E V K E D I G V G S Y S V C K R C I H K A T N M E F A V K I I D K S K R D P T E E I E I L L R Y G Q H P N I I T L K D V Y D D G K Y V Y V V T E L M K G G E L L D K I L R Q K F F S E R E A S A V L F T I T K T V E Y L H A Q G V V H R D L K P S N I L Y V D E S G N P E S I R I C D F G F A K Q L R A E N G L L M T P C Y T A N F V A P E V L K R Q G Y D A A C D I W S L G V L L Y T M L T G Y T P F A N G P D D T P E E I L A R I G S G K F S L S G G Y W N S V S D T A K D L V S K M L H V D P H Q R L T A A L V L R H P W I V H W D Q L P Q Y Q L N R Q D A P H L V K G A M A A T Y S A L N R N Q S P V L E P V G R S T L A Q R R G I K K I T S T A L
- Accession
- P51812
- Protein length
Full Length
- Predicted molecular weight
84 kDa
- Amino acids
1 to 740
- Nature
Recombinant
- Tags
GST tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
Affinity purified.
General info
- Function
Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1 (PubMed:16213824, PubMed:16223362, PubMed:17360704, PubMed:9770464). In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes (PubMed:10436156, PubMed:9770464). In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP (PubMed:16223362). Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity (PubMed:8250835). Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex (PubMed:17360704). In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation (PubMed:18508509, PubMed:18813292). Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway (PubMed:18722121). Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function (PubMed:16213824). Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4) (PubMed:18508509, PubMed:18813292). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression (By similarity). In LPS-stimulated dendritic cells, is involved in TLR4-induced macropinocytosis, and in myeloma cells, acts as effector of FGFR3-mediated transformation signaling, after direct phosphorylation at Tyr-529 by FGFR3 (By similarity). Negatively regulates EGF-induced MAPK1/3 phosphorylation via phosphorylation of SOS1 (By similarity). Phosphorylates SOS1 at 'Ser-1134' and 'Ser-1161' that create YWHAB and YWHAE binding sites and which contribute to the negative regulation of MAPK1/3 phosphorylation (By similarity). Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration (PubMed:26158630). Acts as a regulator of osteoblast differentiation by mediating phosphorylation of ATF4, thereby promoting ATF4 transactivation activity (By similarity).
- Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
- Post-translational modifications
Activated by phosphorylation at Ser-227 by PDPK1. Autophosphorylated on Ser-386, as part of the activation process. May be phosphorylated at Thr-365 and Ser-369 by MAPK1/ERK2 and MAPK3/ERK1. Can also be activated via phosphorylation at Ser-386 by MAPKAPK2.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-80°C
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Rsk 2 also known as MAPKAP Kinase 1b is a 60- to 90-kilodalton serine/threonine kinase belonging to the RSK family. It plays an important role in cell signaling by phosphorylating various substrates. The protein is expressed in many tissues including the brain heart and liver. Rsk 2 contains two distinct catalytic domains which allow it to carry out its functions efficiently. Activation of this protein involves its interaction with ERK/MAPK signaling proteins.
- Biological function summary
Rsk 2 is a central player in the regulation of cell growth survival and motility. It exists as part of the larger MAPK/ERK pathway where it acts downstream of ERK1/2. Upon activation Rsk 2 phosphorylates target proteins which regulate gene expression cell cycle progression and apoptosis. This allows it to control multiple aspects of cell physiology contributing to proper cellular responses to external stimuli.
- Pathways
The protein Rsk 2 integrates into the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are essential for controlling processes such as proliferation and differentiation. Rsk 2 activation depends on upstream kinases like MEK and ERK. In the MAPK/ERK pathway it collaborates with other kinases and transcription factors to mediate cellular responses. This involvement places Rsk 2 at a nexus of signaling events that finely tune cell function and fate.
- Associated diseases and disorders
Rsk 2 mutations can lead to Coffin-Lowry syndrome which is a form of intellectual disability accompanied by physical abnormalities. The protein has also been implicated in certain cancer types whereby its upregulation supports tumor growth and survival. In these contexts interactions between Rsk 2 and proteins such as ERK and AKT contribute to the pathological alterations that occur in these diseases. The ongoing study of Rsk 2 holds potential for understanding and targeting conditions where it plays a role.
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2 product images
SDS-PAGE - Recombinant human Rsk 2 / MAPKAP Kinase 1b (mutated L608F) protein (ab172182)
ab172182 on SDS-PAGE.
Functional Studies - Recombinant human Rsk 2 / MAPKAP Kinase 1b (mutated L608F) protein (ab172182)
Sample Kinase Activity Plot. ab172182 specific activity was determined to be 140 nmol/min/mg.
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