Recombinant human RSPO1 protein (Active)
- Bioactive
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Recombinant human RSPO1 protein (Active) is a Human Fragment protein, in the 31 to 263 aa range, expressed in CHO cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.
View Alternative Names
R-spondin-1, Roof plate-specific spondin-1, hRspo1, RSPO1
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human RSPO1 protein (Active) (AB246420)
SDS-PAGE abalysis of ab246420 (1 μg per lane) under reducing (Lane 1) and non-reducing (Lane 2) conditions.
ab246420 runs at a higher molecular weight than predicted (25.6 kDa) due to glycosylation.
Complementary Products
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RSPO1 acts as a major stimulator of cell proliferation and differentiation. It does not function alone but rather works in association with the Wnt receptors for signal enhancement. RSPO1 positively influences the regenerative processes in tissues and organs contributing to cell fate determination during development. As part of this interaction RSPO1 can reverse the degradation of Wnt receptors therefore amplifying the signaling required for proper developmental processes and tissue maintenance.
Pathways
RSPO1 integrates deeply into the Wnt/β-catenin signaling pathway where its role is important for amplifying the signals important for cellular activities such as proliferation and migration. RSPO1 interacts with pathway members such as Frizzled receptors and lipoprotein receptor-related proteins (Lrp5/6) to potentiate Wnt signaling. The function of RSPO1 in the Wnt pathway aligns with its interplay in epithelial cell stimulation and development making it an essential component in pathways driving cellular growth and differentiation.
Specifications
Form
Lyophilized
General info
Function
Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors (PubMed : 29769720). Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1 (PubMed : 17804805).
Sequence similarities
Belongs to the R-spondin family.
Post-translational modifications
C-, and N-glycosylated. N-glycosylation at Asn-137, negatively influences its secretion and enhancing effect on Wnt/beta-catenin signaling (PubMed:27123103). C-mannosylation at Trp-156 by DPY19L3 is required for its secretion and regulates the enhancing activity of Wnt signaling (PubMed:26764097).
Subcellular localisation
Nucleus
Target data
Product promise
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