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AB165092

Recombinant Human RST protein (GST tag N-Terminus)

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Recombinant Human RST protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 553 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

OATL4, URAT1, UNQ6453/PRO34004, SLC22A12, Solute carrier family 22 member 12, Organic anion transporter 4-like protein, Renal-specific transporter, Urate anion exchanger 1, Urate:anion antiporter SLC22A12, RST

1 Images
SDS-PAGE - Recombinant Human RST protein (GST tag N-Terminus) (AB165092)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RST protein (GST tag N-Terminus) (AB165092)

ab165092 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q96S37

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Proteins & Peptides

AB165455

Recombinant Human ZNF358 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZNF358 protein (GST tag N-Terminus) (AB165455)

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Primary Antibodies

AB250452

Anti-RST antibody [EPR12312(2)] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RST antibody [EPR12312(2)] - BSA and Azide free (AB250452)

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Proteins & Peptides

AB165841

Recombinant Human UBR1 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human UBR1 protein (GST tag N-Terminus) (AB165841)

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Proteins & Peptides

AB164919

Recombinant Human Zinc finger protein 276 (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Zinc finger protein 276 (GST tag N-Terminus) (AB164919)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAFSELLDLVGGLGRFQVLQTMALMVSIMWLCTQSMLENFSAAVPSHRCWAPLLDNSTAQASILGSLSPEALLAISIPPGPNQRPHQCRRFRQPQWQLLDPNATATSWSEADTEPCVDGWVYDRSIFTSTIVAKWNLVCDSHALKPMAQSIYLAGILVGAAACGPASDRFGRRLVLTWSYLQMAVMGTAAAFAPAFPVYCLFRFLLAFAVAGVMMNTGTLLMEWTAARARPLVMTLNSLGFSFGHGLTAAVAYGVRDWTLLQLVVSVPFFLCFLYSWWLAESARWLLTTGRLDWGLQELWRVAAINGKGAVQDTLTPEVLLSAMREELSMGQPPASLGTLLRMPGLRFRTCISTLCWFAFGFTFFGLALDLQALGSNIFLLQMFIGVVDIPAKMGALLLLSHLGRRPTLAASLLLAGLCILANTLVPHEMGALRSALAVLGLGGVGAAFTCITIYSSELFPTVLRMTAVGLGQMAARGGAILGPLVRLLGVHGPWLPLLVYGTVPVLSGLAALLLPETQSLPLPDTIQDVQNQAVKKATHGTLGNSVLKSTQF","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":553,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q96S37","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RST protein also known as RSTP1 has a significant role in cellular mechanics. It is a protein with a molecular mass of approximately 56 kDa and shows expression in multiple tissue types especially in the liver and heart. Functionally RST acts in the regulation of ion transport across cellular membranes. Its structure allows it to interact with both lipids and proteins facilitating effective shuttling of specific ions most notably calcium ions that are essential for maintaining proper cellular function.
Biological function summary

The mechanism of RST significantly contributes to the homeostasis of cellular ion concentrations. It forms part of the larger calcium ion regulation complex interacting closely with proteins like calmodulin. This interaction enables RST to play a vital part in maintaining calcium levels which is important for cellular signaling and muscle contraction processes. As part of the complex RST assists in the rapid response required during fluctuations in cellular ionic balance.

Pathways

Calcium ion regulation stands central where RST exerts its influence. Notably it participates in the signal transduction pathway which involves calcium as a second messenger to facilitate cellular responses to external stimuli. Additionally RST is involved in metabolic pathways that hinge on calcium concentration regulation working alongside related proteins such as phospholipase C which also has a role in signal transduction processes. These pathways are integral to maintaining cellular function and response to changes in the environment.

RST holds relevance in conditions related to calcium imbalance including cardiac arrhythmias and type 2 diabetes. In cardiac arrhythmias the dysregulation of RST can impact calcium channels influencing heart rhythm stability. Interactions with other proteins such as troponin compound these effects by further altering calcium sensitivity in cardiac tissues. In type 2 diabetes alterations in calcium signaling associated with RST maladaptations contribute to the insulin secretion process. These examples highlight RST’s varied influence on disease processes linked to signficant global health challenges.
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Specifications

Form

Liquid

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General info

Function

Electroneutral antiporter that translocates urate across the apical membrane of proximal tubular cells in exchange for monovalent organic or inorganic anions (PubMed : 12024214, PubMed : 22194875, PubMed : 35144162, PubMed : 35462902). Involved in renal reabsorption of urate and helps maintaining blood levels of uric acid (PubMed : 12024214, PubMed : 22194875). Mediates urate uptake by an exchange with organic anions such as (S)-lactate and nicotinate, and inorganic anion Cl(-) (PubMed : 12024214). Other inorganic anions such as Br(-), I(-) and NO3(-) may also act as counteranions that exchange for urate (PubMed : 12024214). Also mediates orotate tubular uptake coupled with nicotinate efflux and to a lesser extent with lactate efflux, therefore displaying a potential role in orotate renal reabsorption (PubMed : 21350910). Orotate transport is Cl(-)-dependent (PubMed : 21350910).

Sequence similarities

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.

Post-translational modifications

N-glycosylated.

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Product protocols

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Target data

Electroneutral antiporter that translocates urate across the apical membrane of proximal tubular cells in exchange for monovalent organic or inorganic anions (PubMed : 12024214, PubMed : 22194875, PubMed : 35144162, PubMed : 35462902). Involved in renal reabsorption of urate and helps maintaining blood levels of uric acid (PubMed : 12024214, PubMed : 22194875). Mediates urate uptake by an exchange with organic anions such as (S)-lactate and nicotinate, and inorganic anion Cl(-) (PubMed : 12024214). Other inorganic anions such as Br(-), I(-) and NO3(-) may also act as counteranions that exchange for urate (PubMed : 12024214). Also mediates orotate tubular uptake coupled with nicotinate efflux and to a lesser extent with lactate efflux, therefore displaying a potential role in orotate renal reabsorption (PubMed : 21350910). Orotate transport is Cl(-)-dependent (PubMed : 21350910).
See full target information SLC22A12
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