Recombinant Human RUNX1T1/ETO/CDR protein is a Human Fragment protein, in the 38 to 604 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M P D S P V D V K T Q S R L T P P T M P P P P T T Q G A P R T S S F T P T T L T N G T S H S P T A L N G A P S P P N G F S N G P S S S S S S S L A N Q Q L P P A C G A R Q L S K L K R F L T T L Q Q F G N D I S P E I G E R V R T L V L G L V N S T L T I E E F H S K L Q E A T N F P L R P F V I P F L K A N L P L L Q R E L L H C A R L A K Q N P A Q Y L A Q H E Q L L L D A S T T S P V D S S E L L L D V N E N G K R R T P D R T K E N G F D R E P L H S E H P S K R P C T I S P G Q R Y S P N N G L S Y Q P N G L P H P T P P P P Q H Y R L D D M A I A H H Y R D S Y R H P S H R D L R D R N R P M G L H G T R Q E E M I D H R L T D R E W A E E W K H L D H L L N C I M D M V E K T R R S L T V L R R C Q E A D R E E L N Y W I R R Y S D A E D L K K G G G S S S S H S R Q Q S P V N P D P V A L D A H R E F L H R P A S G Y V P E E I W K K A E E A V N E V K R Q A M T E L Q K A V S E A E R K A H D M I T T E R A K M E R T V A E A K R Q A A E D A L A V I N Q Q E D S S E S C W N C G R K A S E T C S G C N T A R Y C G S F C Q H K D W E K H H H I C G Q T L Q A Q Q Q G D T P A V S S S V T P N S G A G S P M D T P P A A T P R S T T P G T P S T I E T T P R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:10688654, PubMed:12559562, PubMed:15203199). Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed:23251453). Can repress transactivation mediated by TCF12 (PubMed:16803958). Acts as a negative regulator of adipogenesis (By similarity). The AML1-MTG8/ETO fusion protein frequently found in leukemic cells is involved in leukemogenesis and contributes to hematopoietic stem/progenitor cell self-renewal (PubMed:23812588).
Alternative names
AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2, RUNX1T1, Protein CBFA2T1, Cyclin-D-related protein, Eight twenty one protein, Protein ETO, Protein MTG8, Zinc finger MYND domain-containing protein 2
Recommended products
Recombinant Human RUNX1T1/ETO/CDR protein is a Human Fragment protein, in the 38 to 604 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q06455-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M P D S P V D V K T Q S R L T P P T M P P P P T T Q G A P R T S S F T P T T L T N G T S H S P T A L N G A P S P P N G F S N G P S S S S S S S L A N Q Q L P P A C G A R Q L S K L K R F L T T L Q Q F G N D I S P E I G E R V R T L V L G L V N S T L T I E E F H S K L Q E A T N F P L R P F V I P F L K A N L P L L Q R E L L H C A R L A K Q N P A Q Y L A Q H E Q L L L D A S T T S P V D S S E L L L D V N E N G K R R T P D R T K E N G F D R E P L H S E H P S K R P C T I S P G Q R Y S P N N G L S Y Q P N G L P H P T P P P P Q H Y R L D D M A I A H H Y R D S Y R H P S H R D L R D R N R P M G L H G T R Q E E M I D H R L T D R E W A E E W K H L D H L L N C I M D M V E K T R R S L T V L R R C Q E A D R E E L N Y W I R R Y S D A E D L K K G G G S S S S H S R Q Q S P V N P D P V A L D A H R E F L H R P A S G Y V P E E I W K K A E E A V N E V K R Q A M T E L Q K A V S E A E R K A H D M I T T E R A K M E R T V A E A K R Q A A E D A L A V I N Q Q E D S S E S C W N C G R K A S E T C S G C N T A R Y C G S F C Q H K D W E K H H H I C G Q T L Q A Q Q Q G D T P A V S S S V T P N S G A G S P M D T P P A A T P R S T T P G T P S T I E T T P R
- Accession
- Q06455
- Protein length
- Fragment
- Predicted molecular weight
- 88 kDa
- Amino acids
- 38 to 604
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:10688654, PubMed:12559562, PubMed:15203199). Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed:23251453). Can repress transactivation mediated by TCF12 (PubMed:16803958). Acts as a negative regulator of adipogenesis (By similarity). The AML1-MTG8/ETO fusion protein frequently found in leukemic cells is involved in leukemogenesis and contributes to hematopoietic stem/progenitor cell self-renewal (PubMed:23812588).
- Sequence similarities
Belongs to the CBFA2T family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as RUNX1T1 / ETO.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RUNX1T1 also known as ETO or CDR is an important transcriptional regulator. It has a mass of approximately 70 kDa. This protein is expressed in various body tissues including hematopoietic cells. RUNX1T1 is heavily involved in transcriptional repression playing a role in chromatin remodeling and gene expression regulation. Structurally it binds to DNA through specific interactions which influences gene activation or repression contributing to the regulation of cell differentiation.
- Biological function summary
The functions of RUNX1T1 involve its participation in the formation of transcriptional complexes. It interacts with several co-repressors and co-activators affecting gene transcription which is important for hematopoiesis. It regulates a range of gene expressions minimally necessary for normal blood cell development. These complexes enable RUNX1T1 to function in a tightly controlled way supporting tissue-specific gene expression and ensuring balance in cell proliferation and differentiation.
- Pathways
RUNX1T1 has pivotal roles in the hematopoietic and differentiation pathways. It interacts with RUNX1 forming a RUNX1-RUNX1T1 fusion that disrupts normal blood cell development. This fusion alters its typical role in the regulation of gene transcription which intersects with other signaling pathways such as the TGF-beta pathway. RUNX1T1-ETO fusion is linked with important proteins involved in signaling and transcription processes which demonstrate its broad relevance within cellular growth pathways.
- Associated diseases and disorders
RUNX1T1's misregulation or fusion with RUNX1 characterizes certain leukemias notably acute myeloid leukemia (AML). This fusion protein acts aberrantly in hematopoiesis contributing to the development of leukemic cells. Apart from leukemia abnormalities in RUNX1T1 function can also impact other disorders related with defects in blood cell development. The disease association highlights RUNX1T1's interaction with other oncogenic proteins like FLT3 influencing AML pathogenesis.
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1 product image
SDS-PAGE - Recombinant Human RUNX1T1/ETO/CDR protein (ab152255)
12.5% SDS-PAGE stained with Coomassie Blue.
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