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AB152255

Recombinant Human RUNX1T1/ETO/CDR protein (GST tag N-Terminus)

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Recombinant Human RUNX1T1/ETO/CDR protein (GST tag N-Terminus) is a Human Fragment protein, in the 38 to 604 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2, RUNX1T1, Protein CBFA2T1, Cyclin-D-related protein, Eight twenty one protein, Protein ETO, Protein MTG8, Zinc finger MYND domain-containing protein 2

1 Images
SDS-PAGE - Recombinant Human RUNX1T1/ETO/CDR protein (GST tag N-Terminus) (AB152255)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RUNX1T1/ETO/CDR protein (GST tag N-Terminus) (AB152255)

12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, SDS-PAGE, WB

applications

Biologically active

No

Accession

Q06455

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB195329

Anti-RUNX1T1/ETO/CDR antibody - ChIP Grade

ChIP - Anti-RUNX1T1/ETO/CDR antibody - ChIP Grade (AB195329)

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Proteins & Peptides

AB134873

Recombinant Human RUNX1 / AML1 protein

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Primary Antibodies

AB23980

Anti-RUNX1 / AML1 antibody

Western blot - Anti-RUNX1 / AML1 antibody (AB23980)

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Primary Antibodies

AB76543

Anti-PU.1/Spi1 antibody [EPR3158Y]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] (AB76543)

  • 5
  • 3
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as RUNX1T1 / ETO.
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Sequence info

[{"sequence":"MPDSPVDVKTQSRLTPPTMPPPPTTQGAPRTSSFTPTTLTNGTSHSPTALNGAPSPPNGFSNGPSSSSSSSLANQQLPPACGARQLSKLKRFLTTLQQFGNDISPEIGERVRTLVLGLVNSTLTIEEFHSKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARLAKQNPAQYLAQHEQLLLDASTTSPVDSSELLLDVNENGKRRTPDRTKENGFDREPLHSEHPSKRPCTISPGQRYSPNNGLSYQPNGLPHPTPPPPQHYRLDDMAIAHHYRDSYRHPSHRDLRDRNRPMGLHGTRQEEMIDHRLTDREWAEEWKHLDHLLNCIMDMVEKTRRSLTVLRRCQEADREELNYWIRRYSDAEDLKKGGGSSSSHSRQQSPVNPDPVALDAHREFLHRPASGYVPEEIWKKAEEAVNEVKRQAMTELQKAVSEAERKAHDMITTERAKMERTVAEAKRQAAEDALAVINQQEDSSESCWNCGRKASETCSGCNTARYCGSFCQHKDWEKHHHICGQTLQAQQQGDTPAVSSSVTPNSGAGSPMDTPPAATPRSTTPGTPSTIETTPR","proteinLength":"Fragment","predictedMolecularWeight":"88 kDa","actualMolecularWeight":null,"aminoAcidEnd":604,"aminoAcidStart":38,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q06455","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RUNX1T1 also known as ETO or CDR is an important transcriptional regulator. It has a mass of approximately 70 kDa. This protein is expressed in various body tissues including hematopoietic cells. RUNX1T1 is heavily involved in transcriptional repression playing a role in chromatin remodeling and gene expression regulation. Structurally it binds to DNA through specific interactions which influences gene activation or repression contributing to the regulation of cell differentiation.
Biological function summary

The functions of RUNX1T1 involve its participation in the formation of transcriptional complexes. It interacts with several co-repressors and co-activators affecting gene transcription which is important for hematopoiesis. It regulates a range of gene expressions minimally necessary for normal blood cell development. These complexes enable RUNX1T1 to function in a tightly controlled way supporting tissue-specific gene expression and ensuring balance in cell proliferation and differentiation.

Pathways

RUNX1T1 has pivotal roles in the hematopoietic and differentiation pathways. It interacts with RUNX1 forming a RUNX1-RUNX1T1 fusion that disrupts normal blood cell development. This fusion alters its typical role in the regulation of gene transcription which intersects with other signaling pathways such as the TGF-beta pathway. RUNX1T1-ETO fusion is linked with important proteins involved in signaling and transcription processes which demonstrate its broad relevance within cellular growth pathways.

RUNX1T1's misregulation or fusion with RUNX1 characterizes certain leukemias notably acute myeloid leukemia (AML). This fusion protein acts aberrantly in hematopoiesis contributing to the development of leukemic cells. Apart from leukemia abnormalities in RUNX1T1 function can also impact other disorders related with defects in blood cell development. The disease association highlights RUNX1T1's interaction with other oncogenic proteins like FLT3 influencing AML pathogenesis.
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Specifications

Form

Liquid

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General info

Function

Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed : 10688654, PubMed : 12559562, PubMed : 15203199). Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed : 23251453). Can repress transactivation mediated by TCF12 (PubMed : 16803958). Acts as a negative regulator of adipogenesis (By similarity). The AML1-MTG8/ETO fusion protein frequently found in leukemic cells is involved in leukemogenesis and contributes to hematopoietic stem/progenitor cell self-renewal (PubMed : 23812588).

Sequence similarities

Belongs to the CBFA2T family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed : 10688654, PubMed : 12559562, PubMed : 15203199). Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed : 23251453). Can repress transactivation mediated by TCF12 (PubMed : 16803958). Acts as a negative regulator of adipogenesis (By similarity). The AML1-MTG8/ETO fusion protein frequently found in leukemic cells is involved in leukemogenesis and contributes to hematopoietic stem/progenitor cell self-renewal (PubMed : 23812588).
See full target information RUNX1T1
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