Recombinant Human S100A10 protein (His tag) is a Human Full Length protein, in the 1 to 97 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/g endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/g
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M P S Q M E H A M E T M M F T F H K F A G D K G Y L T K E D L R V L M E K E F P G F L E N Q K D P L A V D K I M K D L D Q C R D G K V G F Q S F F S L I A G L T I A C N D Y F V V H M K Q K G K K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
Alternative names
ANX2LG, CAL1L, CLP11, S100A10, Protein S100-A10, Calpactin I light chain, Calpactin-1 light chain, Cellular ligand of annexin II, S100 calcium-binding protein A10, p10 protein, p11
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Recombinant Human S100A10 protein (His tag) is a Human Full Length protein, in the 1 to 97 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/g endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/g
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P60903-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 500 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 5% Trehalose, 0.87% Sodium chloride, 0.61% Tris buffer
Sequence info
Amino acid sequence
- M P S Q M E H A M E T M M F T F H K F A G D K G Y L T K E D L R V L M E K E F P G F L E N Q K D P L A V D K I M K D L D Q C R D G K V G F Q S F F S L I A G L T I A C N D Y F V V H M K Q K G K K
- Accession
- P60903
- Protein length
- Full Length
- Predicted molecular weight
- 12 kDa
- Amino acids
- 1 to 97
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
Purified by Immobilized metal affinity chromatography.
General info
- Function
Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
- Sequence similarities
Belongs to the S-100 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
S100A10 also known as p11 is a member of the S100 protein family. It has a molecular weight of about 11 kDa. This protein usually exists in the cytoplasm and nucleus of many cell types including epithelial cells and immune cells. S100A10 shows high expression in tissues like lungs kidneys and the intestinal tract. This protein functions mechanically as a calcium-binding protein even though it actually does not bind calcium directly promoting its interaction with other proteins.
- Biological function summary
S100A10 is involved in different cellular processes such as membrane trafficking and cellular signaling. It forms a complex with annexin A2 which is vital for the regulation of processes like endocytosis and exocytosis. This complex helps stabilize plasma membrane phospholipids promoting various transport and signaling mechanisms. S100A10 also plays a role in cellular adhesion and migration connecting with actin cytoskeleton.
- Pathways
S100A10 takes part in pathways linked to cellular migration and angiogenesis. It connects with pathways modulating actin dynamics commonly interacting with other proteins like annexin A2 and ARF6. Additionally S100A10 is linked to the plasminogen activation pathway where it facilitates the conversion of plasminogen to plasmin impacting tissue remodeling processes and endothelial cell function.
- Associated diseases and disorders
S100A10 has been linked to conditions such as cancer and inflammatory diseases. It has an association with annexin A2 in many cancer types suggesting its role in promoting tumor growth and invasion. Additionally S100A10 appears to play a part in depression by influencing serotonin signaling. Changes in S100A10 levels can disrupt cellular environments suggesting its significant link to the pathophysiology of these disorders.
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2 product images
SDS-PAGE - Recombinant Human S100A10 protein (His tag) (ab221399)
SDS-PAGE using ab221399. DTT-reduced protein migrates as 12 kDa.
Western blot - Recombinant Human S100A10 protein (His tag) (ab221399)
**Blocking buffer:** 5% NFDM/TBST
This data was developed using Anti-S100A10 antibody [EPR3317] ab76472, the same antibody clone in a different buffer formulation.Lane 1: Western blot - Anti-S100A10 antibody [EPR3317] (Anti-S100A10 antibody [EPR3317] ab76472) at 1/1000 dilution
Lane 2: Western blot - Anti-S100A10 antibody [EPR3317] (Anti-S100A10 antibody [EPR3317] ab76472) at 1/5000 dilution
All lanes: Western blot - Recombinant Human S100A10 protein (His tag) (ab221399) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa, 25 kDa
Exposure time: 3s
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