Recombinant Human S100A4 Protein is a Human Full Length protein, in the 2 to 101 aa range, expressed in HEK 293, with >=95% purity and <= 0.005 EU/µg endotoxin level.
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Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Biologically active
No
Amino acid sequence
A C P L E K A L D V M V S T F H K Y S G K E G D K F K L N K S E L K E L L T R E L P S F L G K R T D E A A F Q K L M S N L D S N R D N E V D F Q E Y C V F L S C I A M M C N E F F E G F P D K Q P R K K
Target data
Function
Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy (PubMed:16707441, PubMed:23752197, PubMed:30713770). Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (PubMed:16707441). Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (By similarity). Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (PubMed:23752197). Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes (By similarity). In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (PubMed:26654597, PubMed:30713770).
Alternative names
CAPL, MTS1, S100A4, Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4
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Recombinant Human S100A4 Protein is a Human Full Length protein, in the 2 to 101 aa range, expressed in HEK 293, with >=95% purity and <= 0.005 EU/µg endotoxin level.
Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Biologically active
No
- Accession
- P26447-1
- Animal free
No
- Species
Human
- Reconstitution
Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
A C P L E K A L D V M V S T F H K Y S G K E G D K F K L N K S E L K E L L T R E L P S F L G K R T D E A A F Q K L M S N L D S N R D N E V D F Q E Y C V F L S C I A M M C N E F F E G F P D K Q P R K K
- Accession
- P26447
- Protein length
Full Length
- Amino acids
2 to 101
- Nature
Recombinant
Specifications
- Form
Lyophilized
General info
- Function
Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy (PubMed:16707441, PubMed:23752197, PubMed:30713770). Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (PubMed:16707441). Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (By similarity). Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (PubMed:23752197). Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes (By similarity). In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (PubMed:26654597, PubMed:30713770).
- Sequence similarities
Belongs to the S-100 family.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
S100A4 also known as metastasin or MTS1 is a protein with a molecular weight of approximately 12 kDa. It functions by binding calcium which induces conformational changes aiding in its interaction with target proteins. S100A4 is part of the S100 family of proteins characterized by two EF-hand calcium-binding motifs. Expression of S100A4 is observed in various cell types including fibroblasts endothelial cells and several tumor cells where it contributes to processes like cell motility invasion and angiogenesis.
- Biological function summary
The role of S100A4 extends beyond mere calcium binding; it associates with the cytoskeleton promoting cell structures related to movement and metastasis. It does not act alone but often partners with proteins like myosin IIA to regulate cytoskeletal dynamics. Additionally S100A4 modulates extracellular matrix components and interacts with signaling pathways that control cancer progression making it significant in both normal physiological contexts and pathological states.
- Pathways
Functional activities of S100A4 integrate into important pathways involved in cellular movement and metastasis. S100A4 plays roles in the regulation of the Wnt/β-catenin pathway which is pivotal for cell-cell interaction and fate determination. It also interacts with matrix metalloproteinases like MMPs thereby managing processes of tissue invasion and remodeling essential in cancer metastasis. Other pathways influencing cell adhesion and signal transduction may indirectly involve S100A4 through related proteins.
- Associated diseases and disorders
Elevated S100A4 levels have implications in cancer particularly in enhancing metastasis in breast and colorectal cancers. The protein promotes tumor developments by inducing changes in the tumor microenvironment making it a target of interest in oncology. S100A4 also interacts with proteins like p53 and β-catenin which are critical in the pathology of tumors. Moreover S100A4 contributes to fibrotic diseases accentuating tissue remodeling activities by modulating fibroblast behavior and extracellular matrix deposition.
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3 product images
Mass Spectrometry - Recombinant Human S100A4 Protein (ab290059)
Mass determination by ESI-TOF.
Predicted MW is 11654.38 Da. (+/- 10 Da by ESI-TOF). Observed MW is 11398.23 Da.
HPLC - Recombinant Human S100A4 Protein (ab290059)
HPLC analysis of ab290059
SDS-PAGE - Recombinant Human S100A4 Protein (ab290059)
SDS-PAGE analysis of ab290059
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