Recombinant Human S100A5 protein is a Human Full Length protein, in the 1 to 92 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M E T P L E K A L T T M V T T F H K Y S G R E G S K L T L S R K E L K E L I K K E L C L G E M K E S S I D D L M K S L D K N S D Q E I D F K E Y S V F L T M L C M A Y N D F F L E D N K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binds calcium, zinc and copper. One subunit can simultaneously bind 2 calcium ions or 2 copper ions plus 1 zinc ion. Calcium and copper ions compete for the same binding sites.
Alternative names
S100D, S100A5, Protein S100-A5, Protein S-100D, S100 calcium-binding protein A5
Recommended products
Recombinant Human S100A5 protein is a Human Full Length protein, in the 1 to 92 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P33763-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M E T P L E K A L T T M V T T F H K Y S G R E G S K L T L S R K E L K E L I K K E L C L G E M K E S S I D D L M K S L D K N S D Q E I D F K E Y S V F L T M L C M A Y N D F F L E D N K
- Accession
- P33763
- Protein length
- Full Length
- Predicted molecular weight
- 12.9 kDa
- Amino acids
- 1 to 92
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab104750 is purified using conventional chromatography techniques.
General info
- Function
Binds calcium, zinc and copper. One subunit can simultaneously bind 2 calcium ions or 2 copper ions plus 1 zinc ion. Calcium and copper ions compete for the same binding sites.
- Sequence similarities
Belongs to the S-100 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
S100A5 also known as S100 calcium-binding protein A5 is a member of the S100 protein family which plays a role in calcium signal transduction. It has a mass of approximately 10.6 kDa. S100A5 is expressed in various human tissues including the brain where it is found at higher levels than in other tissues. It functions by binding calcium ions which induces a conformational change that allows it to interact with specific target proteins influencing cellular responses.
- Biological function summary
S100A5 influences cellular processes such as proliferation and differentiation. It is not currently known to form part of a protein complex but interacts with other proteins to regulate different cellular functions. The protein's activity is linked to its calcium-dependent conformational change making it responsive to fluctuations in intracellular calcium levels. Scientists have noticed its abundant expression in the brain suggesting a potential role in the regulation of neurological processes.
- Pathways
S100A5 participates in the calcium signaling pathway which is essential for various cellular activities. Through this pathway S100A5 interacts with other S100 family proteins like S100B and S100A1 which share similar functions in calcium handling and signaling. Another pathway showing relevance is the MAPK signaling pathway where S100A5 might play a modulatory role affecting cell survival and apoptosis through calcium-dependent mechanisms.
- Associated diseases and disorders
S100A5 shows associations with neurological disorders due to its expression in the brain and involvement in calcium signaling. Studies suggest a possible link to Alzheimer's disease given the disruption of calcium homeostasis observed in the disorder. Additionally variations in S100A5 expression or function may contribute to schizophrenia's pathophysiology. In both contexts its interaction with other S100 proteins like S100B is important as these proteins collectively affect the central nervous system's function.
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1 product image
SDS-PAGE - Recombinant Human S100A5 protein (ab104750)
15% SDS-PAGE (3μg).
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