Recombinant human SAE1 + UBA2 protein (Active)
Be the first to review this product! Submit a review
|
(0 Publication)
Recombinant human SAE1 + UBA2 protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE, FuncS.
View Alternative Names
AOS1, SUA1, UBLE1A, SAE1, SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A
- FuncS
Supplier Data
Functional Studies - Recombinant human SAE1 + UBA2 protein (Active) (AB268952)
The specific activity of ab268952 was 45 nmol/min/mg in a sumoylation assay using human SUMO2 (1-93) and RanGAP protein as substrate.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human SAE1 + UBA2 protein (Active) (AB268952)
SDS-PAGE analysis of ab268952.
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SAE1 and UBA2 play important roles in cellular processes like DNA repair transcriptional regulation and chromosome segregation. This heterodimer forms the initial step in the SUMOylation cascade which is important for modulating protein interactions locations and stability. These processes regulate gene expression and maintain genomic integrity. By modifying several key proteins SAE1 and UBA2 affect cellular responses to stress and DNA damage.
Pathways
Many cellular mechanisms rely on the SUMOylation pathway that SAE1 and UBA2 facilitate. The SUMOylation pathway interacts closely with the ubiquitin-proteasome system as both systems regulate protein homeostasis. Key proteins such as PIAS and SENP families are also involved in the SUMO pathway either as ligases or proteases and they modulate the effects initiated by SAE1 and UBA2. Additionally the pathway influences the response to cellular stress by modulating transcription factors and DNA repair enzymes.
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Sequence similarities
Belongs to the ubiquitin-activating E1 family.
Subcellular localisation
Nucleus
Target data
Additional targets
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com