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AB140416

Recombinant Human SAE2 / UBA2 protein

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Recombinant Human SAE2 / UBA2 protein is a Human Full Length protein, in the 1 to 640 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, WB.

View Alternative Names

SAE2, UBLE1B, HRIHFB2115, UBA2, SUMO-activating enzyme subunit 2, Anthracycline-associated resistance ARX, Ubiquitin-like 1-activating enzyme E1B, Ubiquitin-like modifier-activating enzyme 2

1 Images
SDS-PAGE - Recombinant Human SAE2 / UBA2 protein (AB140416)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SAE2 / UBA2 protein (AB140416)

SDS-PAGE analysis of ab140416.

Key facts

Purity

>85% Densitometry

Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

Q9UBT2

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Complementary Products

Primary Antibodies

AB185955

Anti-SAE2 / UBA2 antibody [EPR14880]

RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)

  • 4
  • 4
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB140417

Recombinant Human Sumo 1 protein

Western blot - Recombinant Human Sumo 1 protein (AB140417)

  • 0
  • 0
Proteins & Peptides

AB167934

Recombinant Human NCE2/UBE2F protein

SDS-PAGE - Recombinant Human NCE2/UBE2F protein (AB167934)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MALSRGLPRELAEAVAGGRVLVVGAGGIGCELLKNLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFYPKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADVPLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGDIKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDWAEVQSQGEETNASDQQNEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLAEKGDGAELIWDKDDPSAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNIIPAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCALDPPNPNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIEDGKGTILISSEEGETEANNHKKLSEFGIRNGSRLQADDFLQDYTLLINILHSEDLGKDVEFEVVGDAPEKVGPKQAEDAAKSITNGSDDGAQPSTSTAQEQDDVLIVDSDEEDSSNNADVSEEERSRKRKLDEKENLSAKRSRIEQKEELDDVIALD","proteinLength":"Full Length","predictedMolecularWeight":"115 kDa","actualMolecularWeight":null,"aminoAcidEnd":640,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9UBT2","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SAE2 also known as UBA2 is a protein involved mechanically in the SUMO (Small Ubiquitin-like Modifier) conjugation pathway. It functions as an E1 activating enzyme collaborating closely with its partner SAE1 in the initiation of SUMOylation. SAE2 has a known molecular mass of approximately 72 kDa. It is widely expressed in various tissues with high expressions noted in the nucleus. This expression pattern suggests it plays a role in maintaining cellular processes under a variety of conditions.
Biological function summary

This protein plays an important role in the post-translational modification process of SUMOylation which is critical for regulating protein function. SAE2 is part of the E1 activating enzyme complex working specifically with SAE1 to activate and transfer SUMO to target proteins. It contributes to cellular processes such as nuclear-cytosolic transport chromosome segregation and DNA repair. This indicates its functions are central to maintaining genomic stability and cellular homeostasis.

Pathways

SAE2 is integral in the SUMOylation pathway which interacts with critical cellular pathways like the DNA damage response and cell cycle regulation pathways. It has important interactions with proteins such as UBE2I (also known as Ubc9) the only E2 enzyme in the SUMOylation cascade and PIAS family proteins which function as E3 ligases. These connections allow SAE2 to influence various pathways essential for cell survival and proliferation.

SAE2 influences pathological conditions such as cancer and neurodegenerative diseases. Its role in SUMOylation connects it to the regulation of oncogenic pathways potentially linking it with proteins like p53 a well-known tumor suppressor. In neurodegenerative diseases alterations in SUMOylation pathways with SAE2 as a participant might affect proteins involved in neuron function and survival contributing to disease progression. Understanding SAE2's interactions in these diseases might provide insights into therapeutic strategies.
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General info

Function

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Sequence similarities

Belongs to the ubiquitin-activating E1 family.

Post-translational modifications

Sumoylated with SUMO1 and SUMO2/3 and by UBC9. Sumoylation at Lys-236 inhibits enzymatic activity. Sumoylation at the C-terminal lysine cluster plays an essential role in nuclear trafficking.

Subcellular localisation

Nucleus

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Product protocols

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Target data

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
See full target information UBA2
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Product promise

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