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AB99326

Recombinant Human SAHH protein (His tag N-Terminus)

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(2 Publications)

Recombinant Human SAHH protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 432 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec, WB.

View Alternative Names

SAHH, AHCY, Adenosylhomocysteinase, AdoHcyase, S-adenosyl-L-homocysteine hydrolase

2 Images
Western blot - Recombinant Human SAHH protein (His tag N-Terminus) (AB99326)
  • WB

Unknown

Western blot - Recombinant Human SAHH protein (His tag N-Terminus) (AB99326)

All lanes:

Anti-SAHH antibody (<a href='/en-us/products/unavailable/sahh-antibody-ab56146'>ab56146</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human SAHH protein (His tag N-Terminus) (ab99326) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 90s

SDS-PAGE - Recombinant Human SAHH protein (His tag N-Terminus) (AB99326)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SAHH protein (His tag N-Terminus) (AB99326)

15% SDS-PAGE showing ab99326 (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec, WB

applications

Biologically active

No

Accession

P23526

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab99326 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/sahh-antibody-ab56146'>ab56146</a>.</p>" } } }
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Product details

This product was previously labelled as S adenosylhomocysteine hydrolase
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMSDKLPYKVADIGLAAWGRKALDIAENEMPGLMRMRERYSASKPLKGARIAGCLHMTVETAVLIETLVTLGAEVQWSSCNIFSTQDHAAAAIAKAGIPVYAWKGETDEEYLWCIEQTLYFKDGPLNMILDDGGDLTNLIHTKYPQLLPGIRGISEETTTGVHNLYKMMANGILKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVVAGYGDVGKGCAQALRGFGARVIITEIDPINALQAAMEGYEVTTMDEACQEGNIFVTTTGCIDIILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDRYRLKNGRRIILLAEGRLVNLGCAMGHPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMSCDGPFKPDHYRY","proteinLength":"Full Length","predictedMolecularWeight":"49.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":432,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P23526","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S-Adenosylhomocysteine hydrolase also known as SAHH plays a critical mechanical role in cellular metabolism. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (SAH) to homocysteine and adenosine. This enzyme approximately 48 kDa in mass is ubiquitously expressed in most tissues highlighting its universal importance in biological systems. SAHH maintains the cellular methylation balance by regulating the concentrations of SAH a byproduct of methylation reactions.
Biological function summary

S-Adenosylhomocysteine hydrolase acts as an important player in the methylation processes within the cell. By breaking down SAH it ensures proper methylation of DNA proteins and lipids through the recycling of homocysteine and adenosine. SAHH operates as a homotetramer forming a complex essential for its enzymatic function. The balance maintained by SAHH in cellular methylation allows for proper gene expression and signal transduction within various cellular processes.

Pathways

S-Adenosylhomocysteine hydrolase plays an essential role in the methionine cycle and one-carbon metabolism pathways. These pathways are interconnected with the folate cycle important for nucleotide synthesis and cellular methylation. SAHH works with enzymes like methionine synthase and adenosylmethionine linking it to regulation of homocysteine levels. These interconnections are fundamental for maintaining cellular function and homeostasis.

S-Adenosylhomocysteine hydrolase dysregulation can have significant implications. Hyperhomocysteinemia a condition characterized by high levels of homocysteine in the blood often results from SAHH dysfunction leading to cardiovascular diseases. Additionally aberrant SAHH activity connects to certain types of cancer as altered methylation patterns can disrupt normal cell proliferation. The interplay of SAHH with other proteins such as methylenetetrahydrofolate reductase (MTHFR) illustrates the complex network within which this enzyme operates affecting disease outcomes.
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Specifications

Form

Liquid

Additional notes

ab99326 is purified using conventional chromatography techniques.

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General info

Function

Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine (PubMed : 10933798). Binds copper ions (By similarity).

Sequence similarities

Belongs to the adenosylhomocysteinase family.

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Product protocols

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Target data

Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine (PubMed : 10933798). Binds copper ions (By similarity).
See full target information AHCY
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Assay and drug development technologies 12:258-71 PubMed24927133

2014

A sensitive luminescent assay for the histone methyltransferase NSD1 and other SAM-dependent enzymes.

Applications

FuncS

Species

Unspecified reactive species

Katherine M Drake,Venita G Watson,Anne Kisielewski,Rebecca Glynn,Andrew D Napper

Analytical chemistry 84:3593-8 PubMed22416759

2012

Luciferase-based assay for adenosine: application to S-adenosyl-L-homocysteine hydrolase.

Applications

Unspecified application

Species

Unspecified reactive species

Emmanuel S Burgos,Shivali A Gulab,María B Cassera,Vern L Schramm
View all publications
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Secondary Antibodies

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