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AB315375

Recombinant human Salivary Alpha Amylase protein

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(0 Publication)

Recombinant human Salivary Alpha Amylase protein is a Human Fragment protein, in the 15 to 511 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for WB, SDS-PAGE.

View Alternative Names

AMY1, AMY1A, Alpha-amylase 1A, Salivary alpha-amylase

2 Images
Western blot - Recombinant human Salivary Alpha Amylase protein (AB315375)
  • WB

Supplier Data

Western blot - Recombinant human Salivary Alpha Amylase protein (AB315375)

ab315375 used in Western blot.

false

SDS-PAGE - Recombinant human Salivary Alpha Amylase protein (AB315375)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Salivary Alpha Amylase protein (AB315375)

SDS-PAGE analysis of ab315375.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

P0DUB6

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in 10mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage buffer

Preservative: 0.8% Proclin 300 Constituents: 79.82% Trehalose, 14.02% Sodium chloride, 3.86% Tris, 0.46% EDTA, 0.24% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.16% Sodium-N-Lauroylsarcosinate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
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Sequence info

[{"sequence":"AQYSSNTQQGRTSIVHLFEWRWVDIALECERYLAPKGFGGVQVSPPNENVAIHNPFRPWWERYQPVSYKLCTRSGNEDEFRNMVTRCNNVGVRIYVDAVINHMCGNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDGKCKTGSGDIENYNDATQVRDCRLSGLLDLALGKDYVRSKIAEYMNHLIDIGVAGFRIDASKHMWPGDIKAILDKLHNLNSNWFPEGSKPFIYQEVIDLGGEPIKSSDYFGNGRVTEFKYGAKLGTVIRKWNGEKMSYLKNWGEGWGFMPSDRALVFVDNHDNQRGHGAGGASILTFWDARLYKMAVGFMLAHPYGFTRVMSSYRWPRYFENGKDVNDWVGPPNDNGVTKEVTINPDTTCGNDWVCEHRWRQIRNMVNFRNVVDGQPFTNWYDNGSNQVAFGRGNRGFIVFNNDDWTFSLTLQTGLPAGTYCDVISGDKINGNCTGIKIYVSDDGKAHFSISNSAEDPFIAIHAESKL","proteinLength":"Fragment","predictedMolecularWeight":"57.2 kDa","actualMolecularWeight":"43 kDa","aminoAcidEnd":511,"aminoAcidStart":15,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P0DUB6","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1 month
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle|The product can be stored for up to 12 months
False
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Specifications

Form

Lyophilized

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General info

Function

Calcium-binding enzyme that initiates starch digestion in the oral cavity (PubMed : 12527308). Catalyzes the hydrolysis of internal (1->4)-alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins (PubMed : 12527308).

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

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Product protocols

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Target data

Calcium-binding enzyme that initiates starch digestion in the oral cavity (PubMed : 12527308). Catalyzes the hydrolysis of internal (1->4)-alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins (PubMed : 12527308).
See full target information AMY1A
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