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AB271737

Recombinant Human SARM protein (Tagged)

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Recombinant Human SARM protein (Tagged) is a Human Full Length protein in the 28 to 724 aa range with >=90% purity and suitable for western blot and SDS-PAGE.The predicted molecular weight of ab271737 protein is 77 kDa.

- Save time and ensure accurate results - use our recombinant human Human SARM1/SARM protein as a control

View Alternative Names

KIAA0524, SAMD2, SARM, SARM1, NAD(+) hydrolase SARM1, NADase SARM1, hSARM1, NADP(+) hydrolase SARM1, Sterile alpha and Armadillo repeat protein, Sterile alpha and TIR motif-containing protein 1, Sterile alpha motif domain-containing protein 2, Tir-1 homolog, MyD88-5, SAM domain-containing protein 2, HsTIR

2 Images
Western blot - Recombinant Human SARM protein (Tagged) (AB271737)
  • WB

Supplier Data

Western blot - Recombinant Human SARM protein (Tagged) (AB271737)

Recombinant Human SARM protein (Tagged) (ab271737) used as positive control in WB.

All lanes:

Western blot - Recombinant Human SARM protein (Tagged) (ab271737)

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SDS-PAGE - Recombinant Human SARM protein (Tagged) (AB271737)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human SARM protein (Tagged) (AB271737)

SDS-PAGE analysis of ab271737.

Key facts

Purity

>90% SDS-PAGE

Expression system

HEK 293 cells

Tags

DDDDK tag N-Terminus

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

Q6SZW1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Ensure the validity of your result using our recombinant human SARM1/SARM ab271737 as positive control in SDS-PAGE and western blot.


Check out our protein gel staining guide for SDS-PAGE here

Check out our western blot protocol for more information here
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"77 kDa","actualMolecularWeight":null,"aminoAcidEnd":724,"aminoAcidStart":28,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q6SZW1","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The sterile alpha and HEAT/Armadillo motif-containing protein commonly known as SARM is a member of the death domain superfamily. It has a molecular mass of approximately 68 kDa. SARM is expressed in the nervous system and various immune cells. Mechanically SARM functions as an adaptor protein involved in signaling pathways related to immunity and neuroprotection. It primarily modulates signaling cascades by interacting with other key proteins within the cellular environment.
Biological function summary

SARM engages in processes that are important to maintaining neurological health and regulating immune responses. It serves as a part of the innate immunity complex where it contributes to the regulation of inflammatory responses. SARM can also influence mitochondrial function indicating it plays a significant role in cellular energy management and apoptosis control. These multifaceted functions highlight its engagement in complex and dynamic cellular processes.

Pathways

SARM plays an important role in the toll-like receptor (TLR) signaling pathway and mitochondrial apoptosis pathway. SARM interacts closely with TLRs to modulate immune responses particularly impacting the production of type I interferons. In the mitochondrial apoptosis pathway SARM relates to proteins like TRAF6 impacting cell death and survival. Its involvement in these pathways reflects its essential function in controlling cellular stress responses.

SARM has been implicated in neurological diseases such as Alzheimer's disease and infectious diseases such as viral encephalitis. In Alzheimer's disease SARM interacts with other proteins like amyloid precursor protein (APP) modulating pathways that may exacerbate neurodegeneration. In viral encephalitis SARM mediates immune responses providing a link to immune-related proteins like MyD88 which contribute to neuronal damage during infection. This makes SARM a target of interest for therapeutic interventions in both neurological and infectious diseases.
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Specifications

Form

Liquid

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General info

Function

NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism (PubMed : 25908823, PubMed : 27671644, PubMed : 28334607). Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site (PubMed : 15123841, PubMed : 16964262, PubMed : 20306472, PubMed : 25908823). Wallerian degeneration is triggered by NAD(+) depletion : in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide; NAD(+) cleavage promoting cytoskeletal degradation and axon destruction (PubMed : 25908823, PubMed : 28334607, PubMed : 30333228, PubMed : 31128467, PubMed : 31439792, PubMed : 31439793, PubMed : 32049506, PubMed : 32828421, PubMed : 33053563). Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules (PubMed : 29395922). Can activate neuronal cell death in response to stress (PubMed : 20306472). Regulates dendritic arborization through the MAPK4-JNK pathway (By similarity). Involved in innate immune response : inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38 (PubMed : 16964262).

Sequence similarities

Belongs to the SARM1 family.

Post-translational modifications

Phosphorylation at Ser-548 by JNK kinases (MAPK8, MAPK9 and /or MAPK10) enhance the NAD(+) hydrolase (NADase) activity (PubMed:30333228). Phosphorylation at Ser-548 and subsequent activation takes place in response to oxidative stress conditions and inhibits mitochondrial respiration (PubMed:30333228).

Subcellular localisation

Mitochondrion

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Target data

NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism (PubMed : 25908823, PubMed : 27671644, PubMed : 28334607). Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site (PubMed : 15123841, PubMed : 16964262, PubMed : 20306472, PubMed : 25908823). Wallerian degeneration is triggered by NAD(+) depletion : in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide; NAD(+) cleavage promoting cytoskeletal degradation and axon destruction (PubMed : 25908823, PubMed : 28334607, PubMed : 30333228, PubMed : 31128467, PubMed : 31439792, PubMed : 31439793, PubMed : 32049506, PubMed : 32828421, PubMed : 33053563). Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules (PubMed : 29395922). Can activate neuronal cell death in response to stress (PubMed : 20306472). Regulates dendritic arborization through the MAPK4-JNK pathway (By similarity). Involved in innate immune response : inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38 (PubMed : 16964262).
See full target information SARM1
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