Recombinant Human SAT2 protein is a Human Fragment protein, in the 1 to 190 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A S V R I R E A K E G D C G D I L R L I R E L A E F E K L S D Q V K I S E E A L R A D G F G D N P F Y H C L V A E I L P A P G K L L G P C V V G Y G I Y Y F I Y S T W K G R T I Y L E D I Y V M P E Y R G Q G I G S K I I K K V A E V A L D K G C S Q F R L A V L D W N Q R A M D L Y K A L G A Q D L T E A E G W H F F C F Q G E A T R K L A G K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the N-acetylation of the amino acid thialysine (S-(2-aminoethyl)-L-cysteine), a L-lysine analog with the 4-methylene group substituted with a sulfur (PubMed:15283699). May also catalyze acetylation of polyamines, such as norspermidine, spermidine or spermine (PubMed:12803540). However, ability to acetylate polyamines is weak, suggesting that it does not act as a diamine acetyltransferase in vivo (PubMed:15283699).
Alternative names
SSAT2, SAT2, Thialysine N-epsilon-acetyltransferase, Diamine acetyltransferase 2, Spermidine/spermine N(1)-acetyltransferase 2, SSAT-2
Recommended products
Recombinant Human SAT2 protein is a Human Fragment protein, in the 1 to 190 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q96F10-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A S V R I R E A K E G D C G D I L R L I R E L A E F E K L S D Q V K I S E E A L R A D G F G D N P F Y H C L V A E I L P A P G K L L G P C V V G Y G I Y Y F I Y S T W K G R T I Y L E D I Y V M P E Y R G Q G I G S K I I K K V A E V A L D K G C S Q F R L A V L D W N Q R A M D L Y K A L G A Q D L T E A E G W H F F C F Q G E A T R K L A G K
- Accession
- Q96F10
- Protein length
- Fragment
- Predicted molecular weight
- 21 kDa
- Amino acids
- 1 to 190
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
purified by using conventional chromatography techniques.
General info
- Function
Catalyzes the N-acetylation of the amino acid thialysine (S-(2-aminoethyl)-L-cysteine), a L-lysine analog with the 4-methylene group substituted with a sulfur (PubMed:15283699). May also catalyze acetylation of polyamines, such as norspermidine, spermidine or spermine (PubMed:12803540). However, ability to acetylate polyamines is weak, suggesting that it does not act as a diamine acetyltransferase in vivo (PubMed:15283699).
- Sequence similarities
Belongs to the acetyltransferase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SAT2 also known as SLC38A2 is a sodium-coupled neutral amino acid transporter. This protein has a mass of approximately 56 kDa. SAT2 is mainly expressed in the brain and skeletal muscles but also appears in other tissues like the placenta and testis. It transports small neutral amino acids particularly in conditions where high affinity for L-glutamine and L-alanine is needed. SAT2 plays a role in cellular uptake of these amino acids facilitating their movement across cell membranes in a sodium-dependent manner.
- Biological function summary
SAT2 functions as part of the system A amino acid transporter family which is important for amino acid homeostasis. It actively transports amino acids that are used to synthesize proteins and other biologically active molecules. In the central nervous system SAT2 helps maintain glutamine levels for neurotransmitter cycling and energy metabolism. It does not form part of a larger complex but interacts closely with intracellular and extracellular environments to facilitate its transport role.
- Pathways
SAT2 engages in amino acid transport processes integral to nitrogen metabolism and neurotransmitter cycling. In the mTOR signaling pathway SAT2 contributes by supplying necessary amino acids that serve as signaling molecules to regulate cell growth and metabolism. It closely interacts with other amino acid transporters like LAT1 which together maintain balanced amino acid availability necessary for cellular signaling and metabolic needs.
- Associated diseases and disorders
SAT2 plays a role in metabolic conditions like glutamine deficiency where disrupted glutamine transport affects brain and muscle function. Abnormal SAT2 function associates with neurological disorders potentially impacting proteins involved in neurotransmitter synthesis like glutamine synthetase. Such disruptions can have implications for conditions such as epilepsy where altered glutamine and glutamate metabolism may influence seizure activities.
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1 product image
SDS-PAGE - Recombinant Human SAT2 protein (ab124307)
ab124307 at 3 μg analysed by 15% SDS-PAGE.
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