Recombinant human SBK1 protein is a Human Full Length protein, in the 1 to 424 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
>90% Densitometry
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
Amino acid sequence
M S V G C P E P E P P R S L T C C G P G T A P G P G A G V P L L T E D M Q A L T L R T L A A S D V T K H Y E L V R E L G K G T Y G K V D L V V Y K G T G T K M A L K F V N K S K T K L K N F L R E V S I T N S L S S S P F I I K V F D V V F E T E D C Y V F A Q E Y A P A G D L F D I I P P Q V G L P E D T V K R C V Q Q L G L A L D F M H G R Q L V H R D I K P E N V L L F D R E C R R V K L A D F G M T R R V G C R V K R V S G T I P Y T A P E V C Q A G R A D G L A V D T G V D V W A F G V L I F C V L T G N F P W E A A S G A D A F F E E F V R W Q R G R L P G L P S Q W R R F T E P A L R M F Q R L L A L E P E R R G P A K E V F R F L K H E L T S E L R R R P S H R A R K P P G D R P P A A G P L R L E A P G P L K R T V L T E S G S G S R P A P P A V G S V P L P V P V P V P V P V P V P V P E P G L A P Q G P P G R T D G R A D K S K G Q V V L A T A I E I C V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May be involved in signal-transduction pathways related to the control of brain development.
Alternative names
Serine/threonine-protein kinase SBK1, SH3-binding kinase 1, SBK1
Recommended products
Recombinant human SBK1 protein is a Human Full Length protein, in the 1 to 424 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
Alternative names
Serine/threonine-protein kinase SBK1, SH3-binding kinase 1, SBK1
Key facts
- Purity
>90% Densitometry
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
The specific activity of ab204137 was determined to be 20 nmol /min/mg.
- Accession
- Q52WX2-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
M S V G C P E P E P P R S L T C C G P G T A P G P G A G V P L L T E D M Q A L T L R T L A A S D V T K H Y E L V R E L G K G T Y G K V D L V V Y K G T G T K M A L K F V N K S K T K L K N F L R E V S I T N S L S S S P F I I K V F D V V F E T E D C Y V F A Q E Y A P A G D L F D I I P P Q V G L P E D T V K R C V Q Q L G L A L D F M H G R Q L V H R D I K P E N V L L F D R E C R R V K L A D F G M T R R V G C R V K R V S G T I P Y T A P E V C Q A G R A D G L A V D T G V D V W A F G V L I F C V L T G N F P W E A A S G A D A F F E E F V R W Q R G R L P G L P S Q W R R F T E P A L R M F Q R L L A L E P E R R G P A K E V F R F L K H E L T S E L R R R P S H R A R K P P G D R P P A A G P L R L E A P G P L K R T V L T E S G S G S R P A P P A V G S V P L P V P V P V P V P V P V P V P E P G L A P Q G P P G R T D G R A D K S K G Q V V L A T A I E I C V
- Accession
- Protein length
Full Length
- Predicted molecular weight
69 kDa
- Amino acids
1 to 424
- Nature
Recombinant
- Tags
GST tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
Affinity purified.
General info
- Function
May be involved in signal-transduction pathways related to the control of brain development.
- Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
PKI-alpha peptide ab204874 (PKI-alpha peptide) can be utilized as a substrate for assessing kinase activity
Supplementary info
- Activity summary
SBK1 also known as SH3 domain binding kinase 1 is a serine/threonine protein kinase. It measures approximately 110 kDa in mass. SBK1 expresses in various tissues including the brain and testis suggesting its involvement in multiple physiological processes. The kinase activity of SBK1 participates in phosphorylating target proteins influencing cellular functions. Researchers often study SBK1 to understand the regulation of signaling pathways in the nervous system.
- Biological function summary
SBK1 plays important roles in the regulation of neuronal development and synaptic function. It often associates with protein complexes that guide cellular signaling and differentiation. The kinase activity impacts the structure and function of synapses affecting how neurons connect and communicate. By interacting with other kinases and signaling molecules SBK1 contributes to the development function and maintenance of neuronal circuits.
- Pathways
SBK1 integrates into vital signaling networks including the MAPK and PI3K-Akt pathways. These pathways manage diverse cellular responses such as growth survival and differentiation. SBK1 interacts with other pathway members such as ERK1/2 in the MAPK pathway coordinating responses that control cell fate and function. By modulating these pathways SBK1 impacts key cellular processes providing insights into its broader physiological roles.
- Associated diseases and disorders
SBK1 relates to neurological conditions and certain cancers. The dysregulation of SBK1 can contribute to disorders like schizophrenia where its altered signaling affects brain function and structure. In cancer overactive SBK1-driven pathways influence cell proliferation and survival contributing to tumor development. Through these conditions SBK1 intersects with proteins like AKT indicating its significant impact in disease progression and as a potential therapeutic target.
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4 product images
Functional Studies - Recombinant human SBK1 protein (ab204137)
The specific activity of SBK1 (ab204137) was determined to be 17 nmol/min/mg as per activity assay protocol
SDS-PAGE - Recombinant human SBK1 protein (ab204137)
SDS PAGE analysis of ab204137
Functional Studies - Recombinant human SBK1 protein (ab204137)
Sample Kinase Activity Plot showing ab204137. The specific activity of SBK1 was determined to be 20 nmol /min/mg.
SDS-PAGE - Recombinant human SBK1 protein (ab204137)
SDS-PAGE showing ab204137.
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