Recombinant human SCF protein (Active)
- Premium Bioactive
- What is this?
Be the first to review this product! Submit a review
|
(0 Publication)
Recombinant human SCF protein (Active) is a Human Fragment protein, in the 26 to 190 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, Cell Culture, HPLC.
View Alternative Names
MGF, SCF, KITLG, Kit ligand, Mast cell growth factor, Stem cell factor, c-Kit ligand
- Mass Spec
Supplier Data
Mass Spectrometry - Recombinant human SCF protein (Active) (AB259391)
M + 0. 9 Da (calc mass 18586.85)
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water : FA (99.9 : 0.1 v/v) and 15 % acetonitrile : FA (90 : 9.9 : 0.1 v/v/v) to 55 % water : FA (99.9 : 0.1 v/v) and 45 % acetonitrile : FA (90 : 9.9 : 0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).
- FuncS
Lab
Functional Studies - Recombinant human SCF protein (Active) (AB259391)
Fully biologically active when compared to standard. Determined by dose-dependant stimulation of human TF-1 cells. ED50 is ≤ 0.9601 ng /ml, corresponding to a specific activity of 1.04 x 106 units/mg.
- SDS-PAGE
Lab
SDS-PAGE - Recombinant human SCF protein (Active) (AB259391)
SDS-PAGE analysis of ab259391.
- HPLC
Lab
HPLC - Recombinant human SCF protein (Active) (AB259391)
Purity : 95%
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water : TFA (99.9 : 0.1 v/v) and 20 % acetonitrile : water : TFA (90 : 9.9 : 0.1 v/v/v) to 20 % water : TFA (99.9 : 0.1 v/v) and 80 % acetonitrile : water : TFA (90 : 9.9 : 0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SCF acts as an indispensable growth factor promoting hematopoiesis and melanogenesis. It plays a significant role in the regulation of hematopoietic stem cells and mast cells. SCF and its receptor c-KIT form a complex that activates signaling pathways essential for these processes. This protein is also involved in gametogenesis by aiding the proliferation and survival of primordial germ cells. SCF's action is not limited to mammals as studies demonstrate its activity in other species like mouse SCF.
Pathways
SCF impacts multiple important biological systems by engaging with c-KIT. The SCF/c-KIT interaction contributes to the MAPK/ERK pathway influencing cell division and growth. It also plays a role in the PI3K/AKT pathway that governs cell survival. Beyond its direct association with c-KIT SCF's involvement in these pathways connects it to several other proteins like RAS and PI3K which are important for signal transduction.
Specifications
Form
Lyophilized
Additional notes
Purity by HPLC >= 95%.
General info
Function
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
Sequence similarities
Belongs to the SCF family.
Post-translational modifications
A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.. Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.. A soluble form exists as a cleavage product of the extracellular domain.
Subcellular localisation
Cytoskeleton
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com