Recombinant Human SCO2 protein is a Human Full Length protein, in the 42 to 266 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G P A E T G G Q G Q P Q G P G L R T R L L I T G L F G A G L G G A W L A L R A E K E R L Q Q Q K R T E A L R Q A A V G Q G D F H L L D H R G R A R C K A D F R G Q W V L M Y F G F T H C P D I C P D E L E K L V Q V V R Q L E A E P G L P P V Q P V F I T V D P E R D D V E A M A R Y V Q D F H P R L L G L T G S T K Q V A Q A S H S Y R V Y Y N A G P K D E D Q D Y I V D H S I A I Y L L N P D G L F T D Y Y G R S R S A E Q I S D S V R R H M A A F R S V L S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Copper metallochaperone essential for the synthesis and maturation of cytochrome c oxidase subunit II (MT-CO2/COX2). Involved in transporting copper to the Cu(A) site on MT-CO2/COX2 (PubMed:15229189, PubMed:17189203). Also acts as a thiol-disulfide oxidoreductase to regulate the redox state of the cysteines in SCO1 during maturation of MT-CO2/COX2 (PubMed:19336478).
Alternative names
SCO2
Recommended products
Recombinant Human SCO2 protein is a Human Full Length protein, in the 42 to 266 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- O43819-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G P A E T G G Q G Q P Q G P G L R T R L L I T G L F G A G L G G A W L A L R A E K E R L Q Q Q K R T E A L R Q A A V G Q G D F H L L D H R G R A R C K A D F R G Q W V L M Y F G F T H C P D I C P D E L E K L V Q V V R Q L E A E P G L P P V Q P V F I T V D P E R D D V E A M A R Y V Q D F H P R L L G L T G S T K Q V A Q A S H S Y R V Y Y N A G P K D E D Q D Y I V D H S I A I Y L L N P D G L F T D Y Y G R S R S A E Q I S D S V R R H M A A F R S V L S
- Accession
- O43819
- Protein length
- Full Length
- Predicted molecular weight
- 27.4 kDa
- Amino acids
- 42 to 266
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab103492 was purified using conventional chromatography techniques.
General info
- Function
Copper metallochaperone essential for the synthesis and maturation of cytochrome c oxidase subunit II (MT-CO2/COX2). Involved in transporting copper to the Cu(A) site on MT-CO2/COX2 (PubMed:15229189, PubMed:17189203). Also acts as a thiol-disulfide oxidoreductase to regulate the redox state of the cysteines in SCO1 during maturation of MT-CO2/COX2 (PubMed:19336478).
- Sequence similarities
Belongs to the SCO1/2 family.
- Subcellular localisation
- Mitochondrion inner membrane
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Synthesis of cytochrome c oxidase 2 (SCO2) is a mitochondrial protein essential for the formation of cytochrome c oxidase complex also known as complex IV of the electron transport chain. SCO2 has a molecular weight of approximately 29 kDa. This protein is expressed mainly in tissues with high energy demands such as the heart brain and skeletal muscles. It functions within the mitochondria playing a direct role in the maturation of the copper centers of cytochrome c oxidase which is important for the enzyme's proper function.
- Biological function summary
SCO2 ensures efficient oxidative phosphorylation by participating in the assembly and maintenance of complex IV a critical component of the electron transport chain. It associates with other assembly factors and proteins to deliver copper ions to their designated sites within cytochrome c oxidase. By facilitating proper assembly SCO2 supports efficient electron transfer through the respiratory chain ultimately leading to ATP production.
- Pathways
SCO2 is intimately involved in mitochondrial respiratory pathways particularly the oxidative phosphorylation pathway. This pathway is fundamental for ATP production under aerobic conditions. SCO2 interacts with proteins such as COX17 and COX20 which also participate in the copper delivery and assembly of cytochrome c oxidase. This interaction ensures the transfer of electrons from NADH and FADH2 to oxygen a process that generates a proton gradient used by ATP synthase for ATP synthesis.
- Associated diseases and disorders
Defects in SCO2 relate to conditions such as Leigh syndrome and hypertrophic cardiomyopathy. Leigh syndrome a severe neurological disorder emerges from impaired energy production due to dysfunctional cytochrome c oxidase assembly in which defective SCO2 plays a significant part. SCO2 also associates with disorders involving other proteins like SCO1 and SURF1 both of which contribute to the pathology of cytochrome c oxidase deficiencies manifesting in severe metabolic and multisystemic disorders.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human SCO2 protein (ab103492)
15% SDS-PAGE analysis of 3μg ab103492.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com