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AB103492

Recombinant Human SCO2 protein (His tag N-Terminus)

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Recombinant Human SCO2 protein (His tag N-Terminus) is a Human Full Length protein, in the 42 to 266 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

SCO2

1 Images
SDS-PAGE - Recombinant Human SCO2 protein (His tag N-Terminus) (AB103492)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SCO2 protein (His tag N-Terminus) (AB103492)

15% SDS-PAGE analysis of 3μg ab103492.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

O43819

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGPAETGGQGQPQGPGLRTRLLITGLFGAGLGGAWLALRAEKERLQQQKRTEALRQAAVGQGDFHLLDHRGRARCKADFRGQWVLMYFGFTHCPDICPDELEKLVQVVRQLEAEPGLPPVQPVFITVDPERDDVEAMARYVQDFHPRLLGLTGSTKQVAQASHSYRVYYNAGPKDEDQDYIVDHSIAIYLLNPDGLFTDYYGRSRSAEQISDSVRRHMAAFRSVLS","proteinLength":"Full Length","predictedMolecularWeight":"27.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":266,"aminoAcidStart":42,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O43819","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synthesis of cytochrome c oxidase 2 (SCO2) is a mitochondrial protein essential for the formation of cytochrome c oxidase complex also known as complex IV of the electron transport chain. SCO2 has a molecular weight of approximately 29 kDa. This protein is expressed mainly in tissues with high energy demands such as the heart brain and skeletal muscles. It functions within the mitochondria playing a direct role in the maturation of the copper centers of cytochrome c oxidase which is important for the enzyme's proper function.
Biological function summary

SCO2 ensures efficient oxidative phosphorylation by participating in the assembly and maintenance of complex IV a critical component of the electron transport chain. It associates with other assembly factors and proteins to deliver copper ions to their designated sites within cytochrome c oxidase. By facilitating proper assembly SCO2 supports efficient electron transfer through the respiratory chain ultimately leading to ATP production.

Pathways

SCO2 is intimately involved in mitochondrial respiratory pathways particularly the oxidative phosphorylation pathway. This pathway is fundamental for ATP production under aerobic conditions. SCO2 interacts with proteins such as COX17 and COX20 which also participate in the copper delivery and assembly of cytochrome c oxidase. This interaction ensures the transfer of electrons from NADH and FADH2 to oxygen a process that generates a proton gradient used by ATP synthase for ATP synthesis.

Defects in SCO2 relate to conditions such as Leigh syndrome and hypertrophic cardiomyopathy. Leigh syndrome a severe neurological disorder emerges from impaired energy production due to dysfunctional cytochrome c oxidase assembly in which defective SCO2 plays a significant part. SCO2 also associates with disorders involving other proteins like SCO1 and SURF1 both of which contribute to the pathology of cytochrome c oxidase deficiencies manifesting in severe metabolic and multisystemic disorders.
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Specifications

Form

Liquid

Additional notes

ab103492 was purified using conventional chromatography techniques.

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General info

Function

Copper metallochaperone essential for the synthesis and maturation of cytochrome c oxidase subunit II (MT-CO2/COX2). Involved in transporting copper to the Cu(A) site on MT-CO2/COX2 (PubMed : 15229189, PubMed : 17189203). Also acts as a thiol-disulfide oxidoreductase to regulate the redox state of the cysteines in SCO1 during maturation of MT-CO2/COX2 (PubMed : 19336478).

Sequence similarities

Belongs to the SCO1/2 family.

Subcellular localisation

Mitochondrion inner membrane

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Product protocols

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Target data

Copper metallochaperone essential for the synthesis and maturation of cytochrome c oxidase subunit II (MT-CO2/COX2). Involved in transporting copper to the Cu(A) site on MT-CO2/COX2 (PubMed : 15229189, PubMed : 17189203). Also acts as a thiol-disulfide oxidoreductase to regulate the redox state of the cysteines in SCO1 during maturation of MT-CO2/COX2 (PubMed : 19336478).
See full target information Protein SCO2 homolog, mitochondrial
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