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AB135221

Recombinant Human SCYL1 protein

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Recombinant Human SCYL1 protein is a Human Fragment protein, in the 1 to 556 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP, HT019, SCYL1, N-terminal kinase-like protein, Coated vesicle-associated kinase of 90 kDa, SCY1-like protein 1, Telomerase regulation-associated protein, Telomerase transcriptional element-interacting factor, Teratoma-associated tyrosine kinase

1 Images
SDS-PAGE - Recombinant Human SCYL1 protein (AB135221)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SCYL1 protein (AB135221)

SDS-PAGE analysis of ab135221.

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

Q96KG9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MWFFARDPVRDFPFELIPEPPEGGLPGPWALHRGRKKATGSPVSIFVYDVKPGAEEQTQVAKAAFKRFKTLRHPNILAYIDGLETEKCLHVVTEAVTPLGIYLKARVEAGGLKELEISWGLHQIVKALSFLVNDCSLIHNNVCMAAVFVDRAGEWKLGGLDYMYSAQGNGGGPPRKGIPELEQYDPPELADSSGRVVREKWSADMWRLGCLIWEVFNGPLPRAAALRNPGKIPKTLVPHYCELVGANPKVRPNPARFLQNCRAPGGFMSNRFVETNLFLEEIQIKEPAEKQKFFQELSKSLDAFPEDFCRHKVLPQLLTAFEFGNAGAVVLTPLFKVGKFLSAEEYQQKIIPVVVKMFSSTDRAMRIRLLQQMEQFIQYLDEPTVNTQIFPHVVHGFLDTNPAIREQTVKSMLLLAPKLNEANLNVELMKHFARLQAKDEQGPIRCNTTVCLGKIGSYLSASTRHRVLTSAFSRATRDPFAPSRVAGVLGFAATHNLYSMNDCAQKILPVLCGLTVDPEKSVRDQAFKAIRSFLSKLESVSEDPTQLEEVEKDVHA","proteinLength":"Fragment","predictedMolecularWeight":"85 kDa","actualMolecularWeight":null,"aminoAcidEnd":556,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q96KG9","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCYL1 also known as Scy1 Like Pseudokinase 1 is a pseudokinase with a molecular mass of approximately 95 kDa. It localizes primarily to the cytoplasm and is strongly expressed in the central nervous system liver and kidney. The protein possesses several phosphorylation sites and a characteristic N-terminal kinase-like domain although it lacks the typical catalytic activity of kinases. SCYL1 appears to be involved in cellular processes related to protein trafficking and stability.
Biological function summary

SCYL1 contributes to the sorting and transport of proteins between the Golgi apparatus and the endoplasmic reticulum. It interacts with coatomer complexes to facilitate vesicular trafficking ensuring proper cellular function and protein homeostasis. As part of the cellular transport machinery SCYL1 helps maintain the structural integrity of the Golgi complex and likely plays role in neurodevelopment due to its expression patterns and involvement in protein handling.

Pathways

SCYL1 influences the Golgi-to-ER transport pathways and is involved in vesicle formation and movement. It works alongside proteins like coatomer protein complex subunits to maintain efficient vesicular transport within the cell. Through these pathways SCYL1 connects to broader cellular functions like protein sorting and endosome-to-Golgi retrieval contributing to the systematic recycling and allocation of proteins across the cellular framework.

SCYL1 mutations link the protein to spinocerebellar ataxia type 21 and the hepatic disorder 3C syndrome. These conditions highlight the protein's connections to neurological and liver functions reflecting its significant role in these organs. Additionally interactions with proteins like TDP-43 suggest potential contributions to neurodegenerative processes when misregulated. Understanding SCYL1's role in these diseases provides insight into its importance for normal cellular functioning and the consequences of its dysfunction.
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Specifications

Form

Liquid

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General info

Function

Regulates COPI-mediated retrograde protein traffic at the interface between the Golgi apparatus and the endoplasmic reticulum (PubMed : 18556652). Involved in the maintenance of the Golgi apparatus morphology (PubMed : 26581903). Has no detectable kinase activity in vitro (PubMed : 18556652).. Isoform 6 acts as a transcriptional activator. It binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. It also binds to the TERT promoter region.

Sequence similarities

Belongs to the protein kinase superfamily.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Regulates COPI-mediated retrograde protein traffic at the interface between the Golgi apparatus and the endoplasmic reticulum (PubMed : 18556652). Involved in the maintenance of the Golgi apparatus morphology (PubMed : 26581903). Has no detectable kinase activity in vitro (PubMed : 18556652).. Isoform 6 acts as a transcriptional activator. It binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. It also binds to the TERT promoter region.
See full target information SCYL1
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