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Recombinant human SDF1 protein (Active) is a Human Fragment protein, in the 22 to 89 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, Cell Culture, HPLC.

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Images

Functional Studies - Recombinant human SDF1 protein (Active) (AB259416), expandable thumbnail
  • SDS-PAGE - Recombinant human SDF1 protein (Active) (AB259416), expandable thumbnail
  • HPLC - Recombinant human SDF1 protein (Active) (AB259416), expandable thumbnail
  • Mass Spectrometry - Recombinant human SDF1 protein (Active) (AB259416), expandable thumbnail

Publications

Key facts

Purity

>=95% SDS-PAGE

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS, MS, Cell Culture, HPLC

Biologically active

Yes

Amino acid sequence

K P V S L S Y R C P C R F F E S H V A R A N V K H L K I L N T P N C A L Q I V A R L K N N N R Q V C I D P K L K W I Q E Y L E K A L N K

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Application

Cell Culture

Reactivity

Reacts

Dilution info

-

Notes

-

Application

HPLC

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner (PubMed:29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells (By similarity).

Alternative names

Recommended products

Recombinant human SDF1 protein (Active) is a Human Fragment protein, in the 22 to 89 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, Cell Culture, HPLC.

Key facts

Purity

>=95% SDS-PAGE

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, FuncS, MS, Cell Culture, HPLC

Biological activity

Fully biologically active determined by the dose dependant migration of THP-1 cells.

ED50 is ≤ 40 ng/mL, corresponding to a specific activity of 2.50 x 104 units/mg.

Accession
P48061-1
Animal free

Yes

Species

Human

Reconstitution

Reconstitute in PBS

Concentration
Loading...
Storage buffer

pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence

K P V S L S Y R C P C R F F E S H V A R A N V K H L K I L N T P N C A L Q I V A R L K N N N R Q V C I D P K L K W I Q E Y L E K A L N K

Accession

P48061

Protein length

Fragment

Predicted molecular weight

8.02 kDa

Amino acids

22 to 89

Nature

Recombinant

Specifications

Form

Lyophilized

Additional notes

>= 95 % HPLC.

General info

Function

Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner (PubMed:29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells (By similarity).

Sequence similarities

Belongs to the intercrine alpha (chemokine CxC) family.

Post-translational modifications

Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment.

This protein could lose the C-terminal Lys, but this does not affect performance.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The stromal cell-derived factor 1 (SDF1) also known as C-X-C motif chemokine 12 (CXCL12) is a chemokine protein that is important in immunological responses and cellular signaling. This protein weighs approximately 8 kDa. SDF1 is largely expressed in bone marrow stroma liver and endothelium of various tissues positioning it as an integral player in cell migration and homing processes. The protein functions as a chemoattractant for lymphocytes promoting cellular trafficking and organ development.

Biological function summary

SDF1 influences the migration and survival of hematopoietic progenitor cells. It plays a pivotal role in heart development angiogenesis and neuronal protein regulation. SDF1 binds with high affinity to its receptor CXCR4 forming a critical signal transduction complex that modulates cellular movement and growth responses. This interaction is important in the regulation of cell positioning and potential pathways of pathological changes.

Pathways

SDF1 has an important role in the chemokine signaling pathway and is involved in the pathways controlling hematopoietic stem cell migration and homing. The interaction between SDF1 and CXCR4 triggers downstream signaling events engaging proteins like PI3K and MAPK which promote cell survival and proliferation. Furthermore the SDF1/CXCR4 axis is central to the vascular endothelial growth factor (VEGF) pathway facilitating angiogenesis and tissue repair mechanisms.

Associated diseases and disorders

SDF1 relates closely to cancer metastasis and HIV infection. The SDF1/CXCR4 interaction acts as a co-receptor for HIV entry into host cells implicating it in viral pathogenesis. Overexpression of SDF1 and its binding partner CXCR4 contributes to tumor growth invasion and metastasis in various cancers by promoting angiogenesis and tumor cell migration. The targeting of the SDF1/CXCR4 axis holds therapeutic potential in cancer treatment and infectious disease management.

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4 product images

  • Functional Studies - Recombinant human SDF1 protein (Active) (ab259416), expandable thumbnail

    Functional Studies - Recombinant human SDF1 protein (Active) (ab259416)

    Fully biologically active determined by the dose dependant migration of THP-1 cells.

    ED50 is ≤ 40 ng/mL, corresponding to a specific activity of 2.50 x 104 units/mg.

  • SDS-PAGE - Recombinant human SDF1 protein (Active) (ab259416), expandable thumbnail

    SDS-PAGE - Recombinant human SDF1 protein (Active) (ab259416)

    SDS-PAGE analysis of ab259416.

  • HPLC - Recombinant human SDF1 protein (Active) (ab259416), expandable thumbnail

    HPLC - Recombinant human SDF1 protein (Active) (ab259416)

    Purity: 98.5%

    The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.

  • Mass Spectrometry - Recombinant human SDF1 protein (Active) (ab259416), expandable thumbnail

    Mass Spectrometry - Recombinant human SDF1 protein (Active) (ab259416)

    (M-Lys) +/- 0.1 Da (C-terminal Lys loss; calc. mass 7892 Da)

    The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).

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