Recombinant human SDF1 protein (Active)

Specifications

Form

Lyophilized

Additional notes

>= HPLC analyses.Sterile filtered.

General info

Function

Chemoattractant active on T-lymphocytes and monocytes but not neutrophils (PubMed : 18802065, PubMed : 39093700). Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis (PubMed : 8752281, PubMed : 18802065, PubMed : 39093700). Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for CXCL12/SDF-1 (PubMed : 16107333, PubMed : 19255243). Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV : ITGB3, ITGA4 : ITGB1 and ITGA5 : ITGB1 in a CXCR4-independent manner (PubMed : 29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase (PubMed : 18802065). Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins (PubMed : 16107333, PubMed : 18802065, PubMed : 19255243, PubMed : 39093700). CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase (PubMed : 18802065). Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1 (PubMed : 8752281). Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation (By similarity). Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells (By similarity).. SDF-1-beta(3-72). Shows a reduced chemotactic activity.. SDF-1-alpha(3-67). Shows a reduced chemotactic activity (PubMed : 14525775). Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites (PubMed : 14525775).

Sequence similarities

Belongs to the intercrine alpha (chemokine CxC) family.

Post-translational modifications

Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.