Recombinant Human SDHA protein (His tag) is a Human Fragment protein, in the 44 to 664 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
S A K V S D S I S A Q Y P V V D H E F D A V V V G A G G A G L R A A F G L S E A G F N T A C V T K L F P T R S H T V A A Q G G I N A A L G N M E E D N W R W H F Y D T V K G S D W L G D Q D A I H Y M T E Q A P A A V V E L E N Y G M P F S R T E D G K I Y Q R A F G G Q S L K F G K G G Q A H R C C C V A D R T G H S L L H T L Y G R S L R Y D T S Y F V E Y F A L D L L M E N G E C R G V I A L C I E D G S I H R I R A K N T V V A T G G Y G R T Y F S C T S A H T S T G D G T A M I T R A G L P C Q D L E F V Q F H P T G I Y G A G C L I T E G C R G E G G I L I N S Q G E R F M E R Y A P V A K D L A S R D V V S R S M T L E I R E G R G C G P E K D H V Y L Q L H H L P P E Q L A T R L P G I S E T A M I F A G V D V T K E P I P V L P T V H Y N M G G I P T N Y K G Q V L R H V N G Q D Q I V P G L Y A C G E A A C A S V H G A N R L G A N S L L D L V V F G R A C A L S I E E S C R P G D K V P P I K P N A G E E S V M N L D K L R F A D G S I R T S E L R L S M Q K S M Q N H A A V F R V G S V L Q E G C G K I S K L Y G D L K H L K T F D R G M V W N T D L V E T L E L Q N L M L C A L Q T I Y G A E A R K E S R G A H A R E D Y K V R I D E Y D Y S K P I Q G Q Q K K P F E E H W R K H T L S Y V D V G T G K V T L E Y R P V I D K T L N E A D C A T V P P A I R S Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:24781757). Can act as a tumor suppressor (PubMed:20484225).
Alternative names
Flavoprotein subunit of complex II, Fp, SDHA, SDH2, SDHF
Recommended products
Recombinant Human SDHA protein (His tag) is a Human Fragment protein, in the 44 to 664 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P31040-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
S A K V S D S I S A Q Y P V V D H E F D A V V V G A G G A G L R A A F G L S E A G F N T A C V T K L F P T R S H T V A A Q G G I N A A L G N M E E D N W R W H F Y D T V K G S D W L G D Q D A I H Y M T E Q A P A A V V E L E N Y G M P F S R T E D G K I Y Q R A F G G Q S L K F G K G G Q A H R C C C V A D R T G H S L L H T L Y G R S L R Y D T S Y F V E Y F A L D L L M E N G E C R G V I A L C I E D G S I H R I R A K N T V V A T G G Y G R T Y F S C T S A H T S T G D G T A M I T R A G L P C Q D L E F V Q F H P T G I Y G A G C L I T E G C R G E G G I L I N S Q G E R F M E R Y A P V A K D L A S R D V V S R S M T L E I R E G R G C G P E K D H V Y L Q L H H L P P E Q L A T R L P G I S E T A M I F A G V D V T K E P I P V L P T V H Y N M G G I P T N Y K G Q V L R H V N G Q D Q I V P G L Y A C G E A A C A S V H G A N R L G A N S L L D L V V F G R A C A L S I E E S C R P G D K V P P I K P N A G E E S V M N L D K L R F A D G S I R T S E L R L S M Q K S M Q N H A A V F R V G S V L Q E G C G K I S K L Y G D L K H L K T F D R G M V W N T D L V E T L E L Q N L M L C A L Q T I Y G A E A R K E S R G A H A R E D Y K V R I D E Y D Y S K P I Q G Q Q K K P F E E H W R K H T L S Y V D V G T G K V T L E Y R P V I D K T L N E A D C A T V P P A I R S Y
- Accession
- Protein length
Fragment
- Predicted molecular weight
84 kDa
- Amino acids
44 to 664
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:24781757). Can act as a tumor suppressor (PubMed:20484225).
- Sequence similarities
Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.
- Post-translational modifications
Phosphorylation at Tyr-215 is important for efficient electron transfer in complex II and the prevention of ROS generation.
- Subcellular localisation
Mitochondrion inner membrane
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Succinate dehydrogenase complex flavoprotein subunit A (SDHA) also known as complex II Fp or SDH2 plays an important role in the mitochondrial electron transport chain and the tricarboxylic acid (TCA) cycle. It functions as a flavoprotein oxidoreductase catalyzing the oxidation of succinate to fumarate. With a molecular mass of approximately 72 kDa SDHA is expressed in the inner mitochondrial membrane of eukaryotic cells where it is a core component of the succinate dehydrogenase complex (SDHC). The complex is essential for cellular respiration and energy production.
- Biological function summary
SDHA participates in the TCA cycle by accepting electrons from succinate which it donates to the coenzyme Q in the electron transport chain. This essential role connects SDHA to the regulation of ATP production in cells. SDHA operates as part of the larger succinate dehydrogenase (SDH) complex which includes other subunits such as SDHB SDHC and SDHD. This structurally integrated multisubunit complex influences mitochondrial integrity and cellular energy homeostasis.
- Pathways
SDHA is deeply involved in the TCA cycle and oxidative phosphorylation pathway. As a part of these pathways it links to other critical enzymes such as fumarase and aconitase working in concert to drive the conversion of biochemical fuel into usable cellular energy. Its interactions with coenzyme Q and cytochrome complex enzymes are important for electron flow and proton gradient formation across the mitochondrial membrane. Such interactions are central to cellular respiration and energy generation.
- Associated diseases and disorders
Mutations in SDHA correlate with various mitochondrial diseases and cancer syndromes. Specifically SDHA mutations have an association with Leigh syndrome and certain types of mitochondrial complex II deficiency. These mutations disrupt the function of the SDH complex causing metabolic imbalances and energy production issues. Furthermore the integral interaction of SDHA with other SDH subunits means that alterations can impact this entire enzymatic complex with implications for cellular respiration and disease progression.
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1 product image
SDS-PAGE - Recombinant Human SDHA protein (His tag) (ab226453)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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