Recombinant Human SDHA protein (Tagged) is a Human Fragment protein, in the 44 to 293 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
Amino acid sequence
S A K V S D S I S A Q Y P V V D H E F D A V V V G A G G A G L R A A F G L S E A G F N T A C V T K L F P T R S H T V A A Q G G I N A A L G N M E E D N W R W H F Y D T V K G S D W L G D Q D A I H Y M T E Q A P A A V V E L E N Y G M P F S R T E D G K I Y Q R A F G G Q S L K F G K G G Q A H R C C C V A D R T G H S L L H T L Y G R S L R Y D T S Y F V E Y F A L D L L M E N G E C R G V I A L C I E D G S I H R I R A K N T V V A T G G Y G R T Y F S C T S A H T S T G D G T A M I T R A G L P C Q D L E F V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:10746566, PubMed:24781757). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity). Can act as a tumor suppressor (PubMed:20484225).
Alternative names
SDH2, SDHF, SDHA, SDH2, SDHF, Flavoprotein subunit of complex II, Malate dehydrogenase [quinone] flavoprotein subunit, Fp
Recommended products
Recombinant Human SDHA protein (Tagged) is a Human Fragment protein, in the 44 to 293 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Accession
- P31040-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
S A K V S D S I S A Q Y P V V D H E F D A V V V G A G G A G L R A A F G L S E A G F N T A C V T K L F P T R S H T V A A Q G G I N A A L G N M E E D N W R W H F Y D T V K G S D W L G D Q D A I H Y M T E Q A P A A V V E L E N Y G M P F S R T E D G K I Y Q R A F G G Q S L K F G K G G Q A H R C C C V A D R T G H S L L H T L Y G R S L R Y D T S Y F V E Y F A L D L L M E N G E C R G V I A L C I E D G S I H R I R A K N T V V A T G G Y G R T Y F S C T S A H T S T G D G T A M I T R A G L P C Q D L E F V
- Accession
- Protein length
Fragment
- Predicted molecular weight
54.1 kDa
- Amino acids
44 to 293
- Nature
Recombinant
- Tags
GST tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:10746566, PubMed:24781757). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity). Can act as a tumor suppressor (PubMed:20484225).
- Sequence similarities
Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.
- Post-translational modifications
Phosphorylation at Tyr-215 is important for efficient electron transfer in complex II and the prevention of ROS generation.
- Subcellular localisation
Mitochondrion inner membrane
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Succinate dehydrogenase complex flavoprotein subunit A (SDHA) also known as complex II Fp or SDH2 plays an important role in the mitochondrial electron transport chain and the tricarboxylic acid (TCA) cycle. It functions as a flavoprotein oxidoreductase catalyzing the oxidation of succinate to fumarate. With a molecular mass of approximately 72 kDa SDHA is expressed in the inner mitochondrial membrane of eukaryotic cells where it is a core component of the succinate dehydrogenase complex (SDHC). The complex is essential for cellular respiration and energy production.
- Biological function summary
SDHA participates in the TCA cycle by accepting electrons from succinate which it donates to the coenzyme Q in the electron transport chain. This essential role connects SDHA to the regulation of ATP production in cells. SDHA operates as part of the larger succinate dehydrogenase (SDH) complex which includes other subunits such as SDHB SDHC and SDHD. This structurally integrated multisubunit complex influences mitochondrial integrity and cellular energy homeostasis.
- Pathways
SDHA is deeply involved in the TCA cycle and oxidative phosphorylation pathway. As a part of these pathways it links to other critical enzymes such as fumarase and aconitase working in concert to drive the conversion of biochemical fuel into usable cellular energy. Its interactions with coenzyme Q and cytochrome complex enzymes are important for electron flow and proton gradient formation across the mitochondrial membrane. Such interactions are central to cellular respiration and energy generation.
- Associated diseases and disorders
Mutations in SDHA correlate with various mitochondrial diseases and cancer syndromes. Specifically SDHA mutations have an association with Leigh syndrome and certain types of mitochondrial complex II deficiency. These mutations disrupt the function of the SDH complex causing metabolic imbalances and energy production issues. Furthermore the integral interaction of SDHA with other SDH subunits means that alterations can impact this entire enzymatic complex with implications for cellular respiration and disease progression.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
SDS-PAGE - Recombinant Human SDHA protein (Tagged) (ab226268)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226268 with 5% enrichment gel and 15% separation gel.
Mass Spectrometry - Recombinant Human SDHA protein (Tagged) (ab226268)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab226268 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) SDHA.
Mass Spectrometry - Recombinant Human SDHA protein (Tagged) (ab226268)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab226268 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) SDHA.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com