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AB104670

Recombinant Human SDOS protein

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Recombinant Human SDOS protein is a Human Full Length protein, in the 1 to 211 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

SDOS, TIRR, NUDT16L1, Tudor-interacting repair regulator protein, NUDT16-like protein 1, Protein syndesmos

1 Images
SDS-PAGE - Recombinant Human SDOS protein (AB104670)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SDOS protein (AB104670)

15% SDS-PAGE showing ab104670 at approximately 25.5kDa (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9BRJ7

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMSTAAVPELKQISRVEAMRLGPGWSHSCHAMLYAANPGQLFGRIPMRFSVLMQMRFDGLLGFPGGFVDRRFWSLEDGLNRVLGLGLGCLRLTEADYLSSHLTEGPHRVVAHLYARQLTLEQLHAVEISAVHSRDHGLEVLGLVRVPLYTQKDRVGGFPNFLSNAFVSTAKCQLLFALKVLNMMPEEKLVEALAAATEKQKKALEKLLPASS","proteinLength":"Full Length","predictedMolecularWeight":"25.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":211,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9BRJ7","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SDOS also known as SDH-dependent oxidoreductase subunit functions as part of the succinate dehydrogenase complex located in the mitochondrial inner membrane. This protein with a mass of approximately 65 kDa is essential for enzymatic activities in the tricarboxylic acid cycle and the electron transport chain. SDOS is highly expressed in tissues with elevated metabolic rates such as the heart liver and skeletal muscles. Its role involves transferring electrons from succinate to coenzyme Q.
Biological function summary

The SDOS protein contributes significantly to cellular respiration and energy production. It participates in the succinate dehydrogenase complex an important component of the citric acid cycle and the mitochondrial electron transport chain. Within the complex SDOS contributes to the oxidation of succinate to fumarate and the concomitant reduction of ubiquinone. This protein interplays with other complex subunits to facilitate efficient electron flow and ATP synthesis.

Pathways

SDOS plays a significant role in the tricarboxylic acid (TCA) cycle and oxidative phosphorylation pathways. These pathways are critical for aerobic respiration and energy production. SDOS interacts with other components of the succinate dehydrogenase complex such as SDHA and SDHB to link the TCA cycle with the electron transport chain. This connection allows cells to maintain an efficient energy balance and respond to metabolic demands.

The SDOS protein is associated with conditions such as mitochondrial diseases and certain cancers. Mutations affecting SDOS can disrupt the function of the succinate dehydrogenase complex leading to a buildup of succinate and contributing to tumorigenesis. Studies have also shown that alterations in the SDOS protein may relate to the development of Leigh syndrome a severe neurological disorder. These disease connections highlight the significance of SDOS in maintaining cellular metabolic functions.
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Specifications

Form

Liquid

Additional notes

ab104670 is purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

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General info

Function

Key regulator of TP53BP1 required to stabilize TP53BP1 and regulate its recruitment to chromatin (PubMed : 28241136). In absence of DNA damage, interacts with the tandem Tudor-like domain of TP53BP1, masking the region that binds histone H4 dimethylated at 'Lys-20' (H4K20me2), thereby preventing TP53BP1 recruitment to chromatin and maintaining TP53BP1 localization to the nucleus (PubMed : 28241136). Following DNA damage, ATM-induced phosphorylation of TP53BP1 and subsequent recruitment of RIF1 leads to dissociate NUDT16L1/TIRR from TP53BP1, unmasking the tandem Tudor-like domain and allowing recruitment of TP53BP1 to DNA double strand breaks (DSBs) (PubMed : 28241136). Binds U8 snoRNA (PubMed : 18820299).

Sequence similarities

Belongs to the Nudix hydrolase family. TIRR subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Key regulator of TP53BP1 required to stabilize TP53BP1 and regulate its recruitment to chromatin (PubMed : 28241136). In absence of DNA damage, interacts with the tandem Tudor-like domain of TP53BP1, masking the region that binds histone H4 dimethylated at 'Lys-20' (H4K20me2), thereby preventing TP53BP1 recruitment to chromatin and maintaining TP53BP1 localization to the nucleus (PubMed : 28241136). Following DNA damage, ATM-induced phosphorylation of TP53BP1 and subsequent recruitment of RIF1 leads to dissociate NUDT16L1/TIRR from TP53BP1, unmasking the tandem Tudor-like domain and allowing recruitment of TP53BP1 to DNA double strand breaks (DSBs) (PubMed : 28241136). Binds U8 snoRNA (PubMed : 18820299).
See full target information NUDT16L1
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