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AB161213

Recombinant Human SEC61B protein (GST tag N-Terminus)

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(1 Publication)

Recombinant Human SEC61B protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 96 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

Protein transport protein Sec61 subunit beta, SEC61B

1 Images
SDS-PAGE - Recombinant Human SEC61B protein (GST tag N-Terminus) (AB161213)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SEC61B protein (GST tag N-Terminus) (AB161213)

ab161213 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

P60468

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB15576

Anti-SEC61B antibody

Immunocytochemistry/ Immunofluorescence - Anti-SEC61B antibody (AB15576)

  • 4
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB174456

Recombinant Human ProSAAS protein (denatured) (His tag N-Terminus)

SDS-PAGE - Recombinant Human ProSAAS protein (denatured) (His tag N-Terminus) (AB174456)

  • 0
  • 0
Proteins & Peptides

AB159886

Recombinant Human ZP2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZP2 protein (GST tag N-Terminus) (AB159886)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGRTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":96,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P60468","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEC61B also known as Sec61 beta subunit or Protein Transport Protein Sec61 subunit beta is a component of the Sec61 complex. It has a mass of approximately 9 kDa. SEC61B is expressed broadly in various tissues but it is found most abundantly in the endoplasmic reticulum (ER) membrane. This protein functions mechanically as a part of the protein translocation machinery that guides nascent polypeptides into or across the ER membrane.
Biological function summary

SEC61B assists in the regulation and positioning of the Sec61 translocon complex which includes alpha and gamma subunits. This protein is not a gatekeeper of the channel but rather stabilizes the complex and influences co-translational and post-translational translocation of proteins. It plays roles in maintaining ER homeostasis and facilitating the integration of newly synthesized proteins into the ER membrane affecting various cellular processes such as secretion and membrane biogenesis.

Pathways

SEC61B operates in the protein translocation pathways within the ER membrane. It is integral to the secretory pathway that moves proteins from the cytoplasm into the ER lumen or membrane. Additionally SEC61B interacts with proteins like ribosome-bound signal recognition particle (SRP) to facilitate accurate protein targeting and translocation. It associates with other ER resident proteins to manage polypeptide folding and processing.

SEC61B has connections to conditions that involve protein misfolding and improperly handled ER stress such as cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis misfolded proteins can trigger ER stress and cause adaptive responses. The involvement of proteins like the SEC61 complex in ameliorating ER stress highlights potential therapeutic targets. Therefore understanding SEC61B function and regulation may provide insights into therapeutic intervention for ER-stress-related disorders.
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Specifications

Form

Liquid

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General info

Function

Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER) (PubMed : 12475939). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides (PubMed : 12475939). The SEC61 channel is also involved in ER membrane insertion of transmembrane proteins : it mediates membrane insertion of the first few transmembrane segments of proteins, while insertion of subsequent transmembrane regions of multi-pass membrane proteins is mediated by the multi-pass translocon (MPT) complex (PubMed : 32820719, PubMed : 36261522). The SEC61 channel cooperates with the translocating protein TRAM1 to import nascent proteins into the ER (PubMed : 19121997).

Sequence similarities

Belongs to the SEC61-beta family.

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Product protocols

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Target data

Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER) (PubMed : 12475939). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides (PubMed : 12475939). The SEC61 channel is also involved in ER membrane insertion of transmembrane proteins : it mediates membrane insertion of the first few transmembrane segments of proteins, while insertion of subsequent transmembrane regions of multi-pass membrane proteins is mediated by the multi-pass translocon (MPT) complex (PubMed : 32820719, PubMed : 36261522). The SEC61 channel cooperates with the translocating protein TRAM1 to import nascent proteins into the ER (PubMed : 19121997).
See full target information SEC61B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 29:3155-3167 PubMed30332321

2018

Emerin induces nuclear breakage in extract and early embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew R Dilsaver,Pan Chen,Trey A Thompson,Traci Reusser,Richik N Mukherjee,John Oakey,Daniel L Levy
View all publications
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Product promise

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