Recombinant Human SEI-1 protein is a Human Full Length protein, in the 1 to 236 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M L S K G L K R K R E E E E E K E P L A V D S W W L D P G H T A V A Q A P P A V A S S S L F D L S V L K L H H S L Q Q S E P D L R H L V L V V N T L R R I Q A S M A P A A A L P P V P S P P A A P S V A D N L L A S S D A A L S A S M A S L L E D L S H I E G L S Q A P Q P L A D E G P P G R S I G G A A P S L G A L D L L G P A T G C L L D D G L E G L F E D I D T S M Y D N E L W A P A S E G L K P G P E D G P G K E E A P E L D E A E L D Y L M D V L V G T Q A L E R P P G P G R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Acts at E2F-responsive promoters as coregulator to integrate signals provided by PHD- and/or bromodomain-containing transcription factors. Stimulates E2F1/TFDP1 transcriptional activity. Renders the activity of cyclin D1/CDK4 resistant to the inhibitory effects of CDKN2A/p16INK4A.
Alternative names
SEI1, TRIPBR1, SERTAD1, SERTA domain-containing protein 1, CDK4-binding protein p34SEI1, Transcriptional regulator interacting with the PHD-bromodomain 1, SEI-1, p34(SEI-1), TRIP-Br1
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Recombinant Human SEI-1 protein is a Human Full Length protein, in the 1 to 236 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9UHV2-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M L S K G L K R K R E E E E E K E P L A V D S W W L D P G H T A V A Q A P P A V A S S S L F D L S V L K L H H S L Q Q S E P D L R H L V L V V N T L R R I Q A S M A P A A A L P P V P S P P A A P S V A D N L L A S S D A A L S A S M A S L L E D L S H I E G L S Q A P Q P L A D E G P P G R S I G G A A P S L G A L D L L G P A T G C L L D D G L E G L F E D I D T S M Y D N E L W A P A S E G L K P G P E D G P G K E E A P E L D E A E L D Y L M D V L V G T Q A L E R P P G P G R
- Accession
- Q9UHV2
- Protein length
- Full Length
- Predicted molecular weight
- 27.3 kDa
- Amino acids
- 1 to 236
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab130022 was purified using conventional chromatography.
General info
- Function
Acts at E2F-responsive promoters as coregulator to integrate signals provided by PHD- and/or bromodomain-containing transcription factors. Stimulates E2F1/TFDP1 transcriptional activity. Renders the activity of cyclin D1/CDK4 resistant to the inhibitory effects of CDKN2A/p16INK4A.
- Post-translational modifications
Polyubiquitinated, which promotes proteasomal degradation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as Sertad1.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SEI-1 also known as p34SEI-1 or CDK4-binding protein p34 is an important protein involved in cell cycle regulation. It has a molecular mass of approximately 31 kDa. Expression of SEI-1 occurs in various tissues with a noticeable presence in proliferative tissues like the thymus spleen and certain cancerous tissues. It exerts its role mechanistically by interacting with cyclin-dependent kinases (CDKs) particularly CDK4 and modulating their activity during cell cycle progression.
- Biological function summary
SEI-1 influences cell cycle regulation and proliferation. It acts as a regulatory factor for the CDK4/Cyclin D1 complex facilitating the G1 to S phase transition in the cell cycle. This protein by interacting directly with CDK4 plays a part in the assembly and activation of CDK4 complexes which are pivotal for proper cell division. SEI-1 also has a role outside the cell cycle like apoptosis regulation hinting at diverse functionality within cellular processes.
- Pathways
SEI-1 sits prominently in the cell cycle control pathway. It associates closely with the retinoblastoma protein (Rb) pathway where its modulation of CDK4 activity influences Rb phosphorylation. SEI-1’s interaction with CDK4 links it to both the MAP kinase (MAPK) and PI3K/Akt signaling pathways impacting cell growth and survival. Through these associations SEI-1 contributes importantly to the regulation of cellular proliferation and survival mechanisms.
- Associated diseases and disorders
SEI-1 shows connections to cancer particularly in its overexpression found in several cancer types such as breast cancer and gliomas. Its ability to amplify CDK4 activity and deregulate the cell cycle links SEI-1 with oncogenesis. Therapeutic interest targets its interaction with CDK4 to control cancer cell proliferation. SEI-1 also associates with diabetes as its pathway involvement connects to insulin signaling potentially influencing insulin resistance. In both conditions the protein may serve as a potential biomarker or target for new therapeutic strategies.
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1 product image
SDS-PAGE - Recombinant Human SEI-1 protein (ab130022)
15% SDS-PAGE analysis of 3 μg ab130022.
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