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AB276312

Recombinant Human Semaphorin 3A protein (Fc Chimera)

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Recombinant Human Semaphorin 3A protein (Fc Chimera) is a Human Fragment protein, in the 26 to 546 aa range, expressed in HEK 293 cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

SEMAD, SEMA3A, Semaphorin-3A, Semaphorin III, Sema III

1 Images
SDS-PAGE - Recombinant Human Semaphorin 3A protein (Fc Chimera) (AB276312)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Semaphorin 3A protein (Fc Chimera) (AB276312)

SDS-PAGE analysis of ab276312

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

Fc tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q14563

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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Primary Antibodies

AB199475

Anti-Semaphorin 3A antibody [EPR19367]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 3A antibody [EPR19367] (AB199475)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB40772

Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (AB40772)

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Primary Antibodies

AB215715

Anti-TGF beta 1 antibody [EPR21143]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-TGF beta 1 antibody [EPR21143] (AB215715)

  • 5
  • 6
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"KNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLYVGAKDHIFSFDLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLKAYNQTHLYACGTGAFHPICTYIEIGHHPEDNIFKLENSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHPIRTEQHDSRWLNDPKFISAHLISESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLVNKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNFKDPKNPVVYGVFTTSSNIFKGSAVCMYSMSDVRRVFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPMNNRPIVIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSIPKETWYDLEEVLLEEMTVFREPTAISAMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSACSRYF","proteinLength":"Fragment","predictedMolecularWeight":"87.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":546,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q14563","tags":[{"tag":"Fc","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Semaphorin 3A also known as SEMA3A is a secreted protein that plays a role as a guidance cue in the nervous system by binding to receptors like neuropilin-1 and plexin-A. It weighs approximately 95 kDa. Expressed mainly in neurons SEMA3A influences neuronal wiring during development. In addition to neural tissues its expression occurs in endothelial cells and immune cells suggesting a role in vascular and immune system functioning.
Biological function summary

Semaphorin 3A influences cell migration axonal guidance and organogenesis. It functions as a repulsive guidance molecule that unfavorably influences the direction of growing axons and migrating neurons. SEMA3A does not act alone but is part of a complex involving neuropilin and plexin for effective signal transduction. These signaling complexes ensure that axonal pathways form correctly and aid in correct neural network formation.

Pathways

Semaphorin 3An importantly integrates into the axon guidance and immune response pathways. In the axon guidance pathway it repulses axons and influences the cytoskeletal dynamics by interacting with proteins such as collapsin response mediator protein (CRMP) and Rac1. SEMA3A participates in immune response through its influence on immune cell migration and positioning ensuring an appropriate immune response.

Anomalies in Semaphorin 3A activity relate to cancer and neuropathic pain. Aberrant SEMA3A expression and signaling can contribute to tumor progression and metastasis often working with neuropilin-1 and vascular endothelial growth factor (VEGF) in cancerous processes. Furthermore changes in SEMA3A signaling pathways have links to neuropathic pain where it collaborates with CRMP-2 to alter pain perception and neuropathic pain pathways.
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Specifications

Form

Lyophilized

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General info

Function

Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.

Sequence similarities

Belongs to the semaphorin family.

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Product protocols

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Target data

Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.
See full target information SEMA3A
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