Recombinant human Semaphorin 3A protein (Fc Chimera Active) is a Human Full Length protein, in the 25 to 771 aa range, expressed in CHO, with >=95% purity and suitable for SDS-PAGE, FuncS, HPLC.
>=95% SDS-PAGE
CHO cells
His tag N-Terminus
SDS-PAGE, FuncS, HPLC
Yes
H H H H H H H H G K N N V P R L K L S Y K E M L E S N N V I T F N G L A N S S S Y H T F L L D E E R S R L Y V G A K D H I F S F D L V N I K D F Q K I V W P V S Y T R R D E C K W A G K D I L K E C A N F I K V L K A Y N Q T H L Y A C G T G A F H P I C T Y I E I G H H P E D N I F K L E N S H F E N G R G K S P Y D P K L L T A S L L I D G E L Y S G T A A D F M G R D F A I F R T L G H H H P I R T E Q H D S R W L N D P K F I S A H L I S E S D N P E D D K V Y F F F R E N A I D G E H S G K A T H A R I G Q I C K N D F G G H R S L V N K W T T F L K A R L I C S V P G P N G I D T H F D E L Q D V F L M N F K D P K N P V V Y G V F T T S S N I F K G S A V C M Y S M S D V R R V F L G P Y A H R D G P N Y Q W V P Y Q G R V P Y P R P G T C P S K T F G G F D S T K D L P D D V I T F A R S H P A M Y N P V F P M N N R P I V I K T D V N Y Q F T Q I V V D R V D A E D G Q Y D V M F I G T D V G T V L K V V S I P K E T W Y D L E E V L L E E M T V F R E P T A I S A M E L S T K Q Q Q L Y I G S T A G V A Q L P L H R C D I Y G K A C A E C C L A R D P Y C A W D G S A C S R Y F P T A K R A T R A Q D I R N G D P L T H C S D L H H D N H H G H S P E E R I I Y G V E N S S T F L E C S P K S Q R A L V Y W Q F Q R R N E E R K E E I R V D D H I I R T D Q G L L L R S L Q Q K D S G N Y L C H A V E H G F I Q T L L K V T L E V I D T E H L E E L L H K D D D G D G S K T K E M S N S M T P S Q K V W Y R D F M Q L I N H P N L N T M D E F C E Q V W K R D R K Q R R Q R P G H T P G N S N K W K H L Q E N K K G R N R R T H E F E R A P R S V G G P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.
Semaphorin-3A, Semaphorin III, Sema III, SEMA3A, SEMAD
Recombinant human Semaphorin 3A protein (Fc Chimera Active) is a Human Full Length protein, in the 25 to 771 aa range, expressed in CHO, with >=95% purity and suitable for SDS-PAGE, FuncS, HPLC.
Semaphorin-3A, Semaphorin III, Sema III, SEMA3A, SEMAD
>=95% SDS-PAGE
CHO cells
His tag N-Terminus
SDS-PAGE, FuncS, HPLC
Yes
Determined by its ability to bind recombinant rat Neuropilin‐1 Fc Chimera in a functional ELISA assay.
No
Human
Lot numbers beginning with GR3223939 should be reconstituted in 1xPBS to 0.1-0.25 mg/ml. Note: Allow the reconstituted vial to sit at room temperature for 1 hour before use. All other lot numbers should be in 20mM Tris, pH 8.5 to a concentration of 0.1-0.25 mg/ml. Note: Allow the reconstituted vial to sit at room temperature for 2 hours before use. If you have any questions regarding the reconstitution of your product, please do not hesitate to contact our Scientific support team.
H H H H H H H H G K N N V P R L K L S Y K E M L E S N N V I T F N G L A N S S S Y H T F L L D E E R S R L Y V G A K D H I F S F D L V N I K D F Q K I V W P V S Y T R R D E C K W A G K D I L K E C A N F I K V L K A Y N Q T H L Y A C G T G A F H P I C T Y I E I G H H P E D N I F K L E N S H F E N G R G K S P Y D P K L L T A S L L I D G E L Y S G T A A D F M G R D F A I F R T L G H H H P I R T E Q H D S R W L N D P K F I S A H L I S E S D N P E D D K V Y F F F R E N A I D G E H S G K A T H A R I G Q I C K N D F G G H R S L V N K W T T F L K A R L I C S V P G P N G I D T H F D E L Q D V F L M N F K D P K N P V V Y G V F T T S S N I F K G S A V C M Y S M S D V R R V F L G P Y A H R D G P N Y Q W V P Y Q G R V P Y P R P G T C P S K T F G G F D S T K D L P D D V I T F A R S H P A M Y N P V F P M N N R P I V I K T D V N Y Q F T Q I V V D R V D A E D G Q Y D V M F I G T D V G T V L K V V S I P K E T W Y D L E E V L L E E M T V F R E P T A I S A M E L S T K Q Q Q L Y I G S T A G V A Q L P L H R C D I Y G K A C A E C C L A R D P Y C A W D G S A C S R Y F P T A K R A T R A Q D I R N G D P L T H C S D L H H D N H H G H S P E E R I I Y G V E N S S T F L E C S P K S Q R A L V Y W Q F Q R R N E E R K E E I R V D D H I I R T D Q G L L L R S L Q Q K D S G N Y L C H A V E H G F I Q T L L K V T L E V I D T E H L E E L L H K D D D G D G S K T K E M S N S M T P S Q K V W Y R D F M Q L I N H P N L N T M D E F C E Q V W K R D R K Q R R Q R P G H T P G N S N K W K H L Q E N K K G R N R R T H E F E R A P R S V G G P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K
Full Length
226.2 kDa
25 to 771
Recombinant
His tag N-Terminus
Lyophilized
>= 95% by HPLC analysis.
Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.
Belongs to the semaphorin family.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Glycosylated, disulfide-linked homodimer of 1,976 amino acid residues with a calculated molecular weight of 226.2 kDa and therefore runs above the 200kDa marker by SDS-PAGE analysis under nonreducing conditions.
When run under reducing conditions, this protein migrates as three distinct bands that, due to glycosylation, run higher than expected at apparent molecular weights of approximately 120-130 kDa, 90-100 kDa, and 35-40 kDa.
Semaphorin 3A also known as SEMA3A is a secreted protein that plays a role as a guidance cue in the nervous system by binding to receptors like neuropilin-1 and plexin-A. It weighs approximately 95 kDa. Expressed mainly in neurons SEMA3A influences neuronal wiring during development. In addition to neural tissues its expression occurs in endothelial cells and immune cells suggesting a role in vascular and immune system functioning.
Semaphorin 3A influences cell migration axonal guidance and organogenesis. It functions as a repulsive guidance molecule that unfavorably influences the direction of growing axons and migrating neurons. SEMA3A does not act alone but is part of a complex involving neuropilin and plexin for effective signal transduction. These signaling complexes ensure that axonal pathways form correctly and aid in correct neural network formation.
Semaphorin 3An importantly integrates into the axon guidance and immune response pathways. In the axon guidance pathway it repulses axons and influences the cytoskeletal dynamics by interacting with proteins such as collapsin response mediator protein (CRMP) and Rac1. SEMA3A participates in immune response through its influence on immune cell migration and positioning ensuring an appropriate immune response.
Anomalies in Semaphorin 3A activity relate to cancer and neuropathic pain. Aberrant SEMA3A expression and signaling can contribute to tumor progression and metastasis often working with neuropilin-1 and vascular endothelial growth factor (VEGF) in cancerous processes. Furthermore changes in SEMA3A signaling pathways have links to neuropathic pain where it collaborates with CRMP-2 to alter pain perception and neuropathic pain pathways.
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