Recombinant Human Semaphorin 7a protein (Fc Chimera His Tag) is a Human Full Length protein, in the 45 to 648 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A D P Q G H L R S G P R I F A V W K G H V G Q D R V D F G Q T E P H T V L F H E P G S S S V W V G G R G K V Y L F D F P E G K N A S V R T V N I G S T K G S C L D K R D C E N Y I T L L E R R S E G L L A C G T N A R H P S C W N L V N G T V V P L G E M R G Y A P F S P D E N S L V L F E G D E V Y S T I R K Q E Y N G K I P R F R R I R G E S E L Y T S D T V M Q N P Q F I K A T I V H Q D Q A Y D D K I Y Y F F R E D N P D K N P E A P L N V S R V A Q L C R G D Q G G E S S L S V S K W N T F L K A M L V C S D A A T N K N F N R L Q D V F L L P D P S G Q W R D T R V Y G V F S N P W N Y S A V C V Y S L G D I D K V F R T S S L K G Y H S S L P N P R P G K C L P D Q Q P I P T E T F Q V A D R H P E V A Q R V E P M G P L K T P L F H S K Y H Y Q K V A V H R M Q A S H G E T F H V L Y L T T D R G T I H K V V E P G E Q E H S F A F N I M E I Q P F R R A A A I Q T M S L D A E R R K L Y V S S Q W E V S Q V P L D L C E V Y G G G C H G C L M S R D P Y C G W D Q G R C I S I Y S S E R S V L Q S I N P A E P H K E C P N P K P D K A P L Q K V S L A P N S R Y Y L S C P M E S R H A T Y S W R H K E N V E Q S C E P G H Q S P N C I L F I E N L T A Q Q Y G H Y F C E A Q E G S Y F R E A Q H W Q L L P E D G I M A E H L L G H A C A L A L E P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of pro-inflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.
Alternative names
CD108, SEMAL, SEMA7A, Semaphorin-7A, CDw108, JMH blood group antigen, John-Milton-Hargen human blood group Ag, Semaphorin-K1, Semaphorin-L, Sema K1, Sema L
Recommended products
Recombinant Human Semaphorin 7a protein (Fc Chimera His Tag) is a Human Full Length protein, in the 45 to 648 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O75326-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A D P Q G H L R S G P R I F A V W K G H V G Q D R V D F G Q T E P H T V L F H E P G S S S V W V G G R G K V Y L F D F P E G K N A S V R T V N I G S T K G S C L D K R D C E N Y I T L L E R R S E G L L A C G T N A R H P S C W N L V N G T V V P L G E M R G Y A P F S P D E N S L V L F E G D E V Y S T I R K Q E Y N G K I P R F R R I R G E S E L Y T S D T V M Q N P Q F I K A T I V H Q D Q A Y D D K I Y Y F F R E D N P D K N P E A P L N V S R V A Q L C R G D Q G G E S S L S V S K W N T F L K A M L V C S D A A T N K N F N R L Q D V F L L P D P S G Q W R D T R V Y G V F S N P W N Y S A V C V Y S L G D I D K V F R T S S L K G Y H S S L P N P R P G K C L P D Q Q P I P T E T F Q V A D R H P E V A Q R V E P M G P L K T P L F H S K Y H Y Q K V A V H R M Q A S H G E T F H V L Y L T T D R G T I H K V V E P G E Q E H S F A F N I M E I Q P F R R A A A I Q T M S L D A E R R K L Y V S S Q W E V S Q V P L D L C E V Y G G G C H G C L M S R D P Y C G W D Q G R C I S I Y S S E R S V L Q S I N P A E P H K E C P N P K P D K A P L Q K V S L A P N S R Y Y L S C P M E S R H A T Y S W R H K E N V E Q S C E P G H Q S P N C I L F I E N L T A Q Q Y G H Y F C E A Q E G S Y F R E A Q H W Q L L P E D G I M A E H L L G H A C A L A L E P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K H H H H H H
- Accession
- O75326
- Protein length
- Full Length
- Predicted molecular weight
- 95.7 kDa
- Amino acids
- 45 to 648
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of pro-inflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.
- Sequence similarities
Belongs to the semaphorin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Semaphorin 7a also known as SEMA7A or CD108 is a glycoprotein that has a molecular mass of approximately 80 kDa. This target plays a role in axonal guidance and immune response regulation. It is a member of the semaphorin family which generally acts as signaling molecules. Semaphorin 7a is expressed in various tissues including the immune system nervous system and some epithelial cells. Its expression is especially significant in T-cells and is involved in modulating cell adhesion and migration.
- Biological function summary
Semaphorin 7a is implicated in diverse processes such as immune system modulation and nervous system development. It functions as part of a complex involving integrins which mediate cell-to-cell communication and interaction with the extracellular matrix. This protein can bridge the immune and neural signaling pathways indicating its multifunctional nature within organisms. Its interaction with β1 integrins enhances T-cell activation and cytokine production which is important for immune response.
- Pathways
Semaphorin 7a integrates into the MAPK and PI3K signaling pathways. These pathways are essential for cell survival migration and apoptosis. In the MAPK pathway Semaphorin 7a impacts downstream signaling through its interaction with ERK proteins. Its role in the PI3K pathway connects it to AKT proteins influencing cell growth and metabolic processes. These connections show its versatility and critical role in signal transduction linked to cellular behaviors.
- Associated diseases and disorders
Semaphorin 7a has been associated with multiple sclerosis and melanoma. In multiple sclerosis altered expression of this protein can affect immune response and neural repair processes. Its connection to melanoma is linked with its role in regulating cell motility and adhesion impacting cancer progression and metastasis. Semaphorin 7a's interactions with proteins like Melanocortin 1 Receptor (MC1R) in melanoma provide insights into therapeutic targets for disease interventions.
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1 product image
SDS-PAGE - Recombinant Human Semaphorin 7a protein (Fc Chimera His Tag) (ab227401)
15% SDS-PAGE analysis of 3 μg ab227401.
MW: 70-100 kDa (SDS-PAGE under reducing conditions).
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