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AB153783

Recombinant Human Semenogelin I protein

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Recombinant Human Semenogelin I protein is a Human Fragment protein, in the 24 to 402 aa range, expressed in HEK 293 cells, with >95%, < 0.1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

View Alternative Names

SEMG, SEMG1, Semenogelin-1, Cancer/testis antigen 103, Semenogelin I, SGI

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 0.1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

HPLC, SDS-PAGE

applications

Biologically active

No

Accession

P04279

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 4.5 Constituents: 99% Sodium acetate, 0.88% Sodium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"QKGGSKGRLPSEFSQFPHGQKGQHYSGQKGKQQTESKGSFSIQYTYHVDANDHDQSRKSQQYDLNALHKTTKSQRHLGGSQQLLHNKQEGRDHDKSKGHFHRVVIHHKGGKAHRGTQNPSQDQGNSPSGKGISSQYSNTEERLWVHGLSKEQTSVSGAQKGRKQGGSQSSYVLQTEELVANKQQRETKNSHQNKGHYQNVVEVREEHSSKVQTSLCPAHQDKLQHGSKDIFSTQDELLVYNKNQHQTKNLNQDQQHGRKANKISYQSSSTEERRLHYGENGVQKDVSQRSIYSQTEKLVAGKSQIQAPNPKQEPWHGENAKGESGQSTNREQDLLSHEQKGRHQHGSHGGLDIVIIEQEDDSDRHLAQHLNNDQNPLFTVDHHHHHH","proteinLength":"Fragment","predictedMolecularWeight":"43.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":402,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P04279","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Semenogelin I sometimes abbreviated as SgI acts mechanically as an important component in the formation of the semen coagulum. This protein has a molecular mass of approximately 52 kDa and is expressed chiefly in the seminal vesicles. After ejaculation Semenogelin I contributes significantly to the formation of a gel-like matrix which plays an important role in the initial viscosity of seminal fluid. Upon the cleavage of Semenogelin I by prostate-specific antigen (PSA) this gel liquefies allowing sperm to achieve motility.
Biological function summary

The primary role of Semenogelin I lies in its involvement in semen coagulation and liquefaction processes. It forms a complex with other seminal plasma proteins to stabilize the gel structure. This structural role ensures that Semenogelin I helps regulate the controlled release and proper function of spermatozoa. Additionally it is implicated in modulating immune system responses against male seminal antigens to prevent adverse reactions in the female reproductive tract.

Pathways

The actions of Semenogelin I integrate it into reproductive and coagulation pathways. In the context of semen coagulation and liquefaction it works closely with PSA which breaks down Semenogelin I and facilitates liquefaction and sperm motility. This process ties Semenogelin I into the prostaglandin signaling pathway which affects reproductive physiology and fertility. Furthermore proteins such as fibronectin and lactoferrin relate to Semenogelin I within these pathways assisting in post-ejaculation changes that enhance sperm function and fertility.

Semenogelin I is linked to fertility issues and prostate cancer. Abnormal levels or altered cleavage patterns can contribute to male infertility potentially through improper semen liquefaction that affects sperm motility. In prostate cancer elevated degradation of Semenogelin I by enzymes like PSA could serve as an indicator of tumor activity and progression. Consequently monitoring Semenogelin I alongside PSA levels can offer insights into both male reproductive health and prostate cancer management.
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Specifications

Form

Lyophilized

Additional notes

ab153783 was determined to be >95% pure by SEC-HPLC and reducing SDS-PAGE.

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General info

Function

Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA.. Alpha-inhibin-92 and alpha-inhibin-31, derived from the proteolytic degradation of semenogelin, inhibit the secretion of pituitary follicle-stimulating hormone.

Sequence similarities

Belongs to the semenogelin family.

Post-translational modifications

Transglutaminase substrate.. Rapidly cleaved after ejaculation by KLK3/PSA, resulting in liquefaction of the semen coagulum and the progressive release of motile spermatozoa.

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Product protocols

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Target data

Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA.. Alpha-inhibin-92 and alpha-inhibin-31, derived from the proteolytic degradation of semenogelin, inhibit the secretion of pituitary follicle-stimulating hormone.
See full target information SEMG1
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