Recombinant human SENP2 protein (Active) is a Human Fragment protein, in the 360 to 589 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
R R T D D L L E L T E D M E K E I S N A L G H G P Q D E I L S S A F K L R I T R G D I Q T L K N Y H W L N D E V I N F Y M N L L V E R N K K Q G Y P A L H V F S T F F Y P K L K S G G Y Q A V K R W T K G V N L F E Q E I I L V P I H R K V H W S L V V I D L R K K C L K Y L D S M G Q K G H R I C E I L L Q Y L Q D E S K T K R N S D L N L L E W T H H S M K P H E I P Q Q L N G S D C G M F T C K Y A D Y I S R D K P I T F T Q H Q M P L F R K K M V W E I L H Q Q L L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Protease that catalyzes two essential functions in the SUMO pathway (PubMed:11896061, PubMed:12192048, PubMed:15296745, PubMed:20194620, PubMed:21965678). The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins (PubMed:15296745). The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein (PubMed:15296745, PubMed:20194620, PubMed:21965678). May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway (By similarity). Deconjugates SUMO2 from MTA1 (PubMed:21965678). Plays a dynamic role in adipogenesis by desumoylating and promoting the stabilization of CEBPB (PubMed:20194620). Acts as a regulator of the cGAS-STING pathway by catalyzing desumoylation of CGAS and STING1 during the late phase of viral infection (By similarity).
Alternative names
KIAA1331, SENP2, Sentrin-specific protease 2, Axam2, SMT3-specific isopeptidase 2, Sentrin/SUMO-specific protease SENP2, Smt3ip2
Recommended products
Recombinant human SENP2 protein (Active) is a Human Fragment protein, in the 360 to 589 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab268954 was ≥3351 nmol/min/mg in a deubiquitinase assay using recombinant human SUMO1-based proluciferin protein as substrate.
- Accession
- Q9HC62-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence
- R R T D D L L E L T E D M E K E I S N A L G H G P Q D E I L S S A F K L R I T R G D I Q T L K N Y H W L N D E V I N F Y M N L L V E R N K K Q G Y P A L H V F S T F F Y P K L K S G G Y Q A V K R W T K G V N L F E Q E I I L V P I H R K V H W S L V V I D L R K K C L K Y L D S M G Q K G H R I C E I L L Q Y L Q D E S K T K R N S D L N L L E W T H H S M K P H E I P Q Q L N G S D C G M F T C K Y A D Y I S R D K P I T F T Q H Q M P L F R K K M V W E I L H Q Q L L
- Accession
- Q9HC62
- Protein length
- Fragment
- Amino acids
- 360 to 589
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Protease that catalyzes two essential functions in the SUMO pathway (PubMed:11896061, PubMed:12192048, PubMed:15296745, PubMed:20194620, PubMed:21965678). The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins (PubMed:15296745). The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein (PubMed:15296745, PubMed:20194620, PubMed:21965678). May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway (By similarity). Deconjugates SUMO2 from MTA1 (PubMed:21965678). Plays a dynamic role in adipogenesis by desumoylating and promoting the stabilization of CEBPB (PubMed:20194620). Acts as a regulator of the cGAS-STING pathway by catalyzing desumoylation of CGAS and STING1 during the late phase of viral infection (By similarity).
- Sequence similarities
Belongs to the peptidase C48 family.
- Post-translational modifications
Polyubiquitinated; which leads to proteasomal degradation.
- Subcellular localisation
- Nucleus, Nuclear pore complex, Nucleus membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SENP2 also known as Sentrin/SUMO-specific protease 2 functions as a protease that removes SUMO (Small Ubiquitin-like Modifier) moieties from target proteins. This activity regulates the sumoylation state of proteins affecting their stability activity and localization. SENP2 has a molecular mass of around 63 kDa. It is widely expressed in various tissues including the heart liver and placenta with particularly high levels in these areas suggesting its importance across different biological processes.
- Biological function summary
SUMO E3 ligase complexes often include SENP2 as an integral part. It modulates various signaling pathways by targeting transcription factors and other proteins involved in cellular stress response cell division and apoptosis. Through de-sumoylation SENP2 influences the activity of proteins by altering their transcriptional potency nuclear transport or protein degradation rates. SENP2's activity ensures appropriate cellular responses to environmental cues and maintains proper cellular homeostasis by balancing protein modifications.
- Pathways
SUMO-related mechanisms like nucleocytoplasmic transport and DNA repair involve SENP2. In these pathways SENP2 interacts with proteins like RanBP2 and PML contributing to cell cycle regulation and maintaining genomic stability. In the hypoxia response pathway SENP2's influence is important for modulating hypoxia-inducible factors (HIFs). These interactions illustrate SENP2’s regulatory roles in adapting cellular function in response to stress and changes in the cellular environment.
- Associated diseases and disorders
SENP2 displays relevance in cancer and cardiovascular disorders. Altered sumoylation activity linked with SENP2 is often observed in certain cancers where it may misregulate transcription factors like p53 and c-Myc. In cardiovascular diseases SENP2 interacts with proteins such as NFATc1 impacting cardiac development and function. These associations highlight SENP2's potential as a therapeutic target where modulation of its activity might offer novel treatment avenues for related diseases.
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2 product images
Functional Studies - Recombinant human SENP2 protein (Active) (ab268954)
The specific activity of ab268954 was ≥3351 nmol/min/mg in a deubiquitinase assay using recombinant human SUMO1-based proluciferin protein as substrate.
SDS-PAGE - Recombinant human SENP2 protein (Active) (ab268954)
SDS-PAGE analysis of ab268954.
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