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AB268955

Recombinant human SENP8 protein (Active)

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Recombinant human SENP8 protein (Active) is a Human Full Length protein, in the 1 to 212 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

DEN1, NEDP1, PRSC2, FKSG8, SENP8, Sentrin-specific protease 8, Deneddylase-1, NEDD8-specific protease 1, Sentrin/SUMO-specific protease SENP8

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Functional Studies - Recombinant human SENP8 protein (Active) (AB268955)
  • FuncS

Supplier Data

Functional Studies - Recombinant human SENP8 protein (Active) (AB268955)

The specific activity of ab268955 was ≥3434 nmol/min/mg in a deubiquitinase assay using recombinant human NEDD8-based proluciferin protein as substrate.

SDS-PAGE - Recombinant human SENP8 protein (Active) (AB268955)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human SENP8 protein (Active) (AB268955)

SDS-PAGE analysis of ab268955.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab268955 was ≥3434 nmol/min/mg in a deubiquitinase assay using recombinant human NEDD8-based proluciferin protein as substrate.

Accession

Q96LD8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MDPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQFIKCTSNPAEIAMFLEPLDLPNKRVVFLAINDNSNQAAGGTHWSLLVYLQDKNSFFHYDSHSRSNSVHAKQVAEKLEAFLGRKGDKLAFVEEKAPAQQNSYDCGMYVICNTEALCQNFFRQQTESLLQLLTPAYITKKRGEWKDLITTLAKK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":212,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96LD8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SENP8 also known as SUMO/Sentrin Specific Peptidase 8 functions as a protein involved in the process of neddylation. SENP8 has a molecular weight of approximately 23 kDa. This protein mediates deneddylation which involves the removal of the small ubiquitin-like protein NEDD8 from its substrates. SENP8 is mainly expressed in tissues where active cell division occurs like spleen thymus and testis.
Biological function summary

SENP8 plays an important role in regulating neddylation influencing protein stability activity and localization. SENP8 operates independently rather than as a part of any larger protein complex. Its activity is important in cell cycle regulation DNA replication and repair. By removing NEDD8 from target proteins SENP8 controls the degradation of certain proteins impacting cellular processes.

Pathways

SENP8 links tightly with the neddylation and ubiquitin-proteasome pathways. These pathways govern protein turnover and degradation impacting cell cycle control and apoptosis. SENP8 influences proteins such as cullins which are core components of the SCF ubiquitin ligase complex. This relationship highlights SENP8's critical role in cellular protein homeostasis and regulation through these pathways.

Dysfunctional SENP8 activity connects to cancer and neurodegenerative diseases. Alterations in SENP8 expression or function can lead to uncontrolled cell growth and proliferation linked to certain cancers. SENP8 also has potential connections to Alzheimer's disease due to its effect on protein stability and degradation involving proteins like cullin-1. Changes in neddylation and proteasome pathways indicate SENP8's influence in these conditions.
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Specifications

Form

Liquid

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General info

Function

Protease that catalyzes two essential functions in the NEDD8 pathway : processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53.

Sequence similarities

Belongs to the peptidase C48 family.

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Product protocols

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Target data

Protease that catalyzes two essential functions in the NEDD8 pathway : processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53.
See full target information SENP8
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