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AB109972

Recombinant Human SEPT5 protein (His tag N-Terminus)

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Recombinant Human SEPT5 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 369 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

PNUTL1, SEPT5, SEPTIN5, Septin-5, Cell division control-related protein 1, Peanut-like protein 1, CDCrel-1

2 Images
SDS-PAGE - Recombinant Human SEPT5 protein (His tag N-Terminus) (AB109972)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human SEPT5 protein (His tag N-Terminus) (AB109972)

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

SDS-PAGE - Recombinant Human SEPT5 protein (His tag N-Terminus) (AB109972)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SEPT5 protein (His tag N-Terminus) (AB109972)

15% SDS-PAGE analysis of 3 μg ab109972.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q99719

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 5 Preservative: 1.702% Imidazole Constituents: 10% Glycerol (glycerin, glycerine), 2.922% Sodium chloride, 0.39% MES, 0.0017% PMSF

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMSTGLRYKSKLATPEDKQDIDKQYVGFATLPNQVHRKSVKKGFDFTLMVAGESGLGKSTLVHSLFLTDLYKDRKLLSAEERISQTVEILKHTVDIEEKGVKLKLTIVDTPGFGDAVNNTECWKPITDYVDQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPVDVGFMKALHEKVNIVPLIAKADCLVPSEIRKLKERIREEIDKFGIHVYQFPECDSDEDEDFKQQDRELKESAPFAVIGSNTVVEAKGQRVRGRLYPWGIVEVENQAHCDFVKLRNMLIRTHMHDLKDVTCDVHYENYRAHCIQQMTSKLTQDSRMESPIPILPLPTPDAETEKLIRMKDEELRRMQEMLQRMKQQMQDQ","proteinLength":"Full Length","predictedMolecularWeight":"45.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":369,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q99719","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEPT5 also known as PNUTL1 or hCNTRL is a protein involved in the mechanics of cellular division and membrane dynamics. It has a molecular mass of about 39 kDa and is predominantly found in neuronal and hematopoietic tissues. SEPT5 functions as part of the septin family which plays a role in cytoskeletal rearrangements. Within cells SEPT5 assembles into filaments essential for maintaining structural integrity during cell division.
Biological function summary

SEPT5 is involved in bundling and organizing actin filaments. It forms part of a larger septin complex which includes other septin proteins like SEPT2 and SEPT7. This complex is responsible for the proper organization and stability of cytoskeletal structures which is important for processes like cytokinesis and exocytosis. SEPT5's ability to bind lipid membranes indicates its role in membrane-trafficking processes. Its interactions with vesicular proteins suggest SEPT5's involvement in neurotransmitter release.

Pathways

SEPT5 plays a role in the cell cycle and synaptic vesicle trafficking pathways. In the cell cycle SEPT5 collaborates with proteins such as Cdc42 and calmodulin to ensure accurate division timing and fidelity. In synaptic vesicle trafficking SEPT5 associates with proteins like syntaxin and SNARE complexes contributing to the regulation of neurotransmitter release. These pathways highlight the importance of SEPT5 in both cell proliferation and nervous system functioning.

SEPT5 has been implicated in conditions like Parkinson's disease and acute myeloid leukemia (AML). In Parkinson's disease SEPT5 may interact with alpha-synuclein which is known to disrupt synaptic function and contribute to the pathogenesis of the disorder. In AML deregulation of SEPT5 expression disturbs normal cell division processes. Its interactions with other septins and cell cycle regulators have implications for understanding its role in these diseases.

Specifications

Form

Liquid

Additional notes

ab109972 was purified using conventional chromatography.

General info

Function

Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion (By similarity).

Sequence similarities

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.

Post-translational modifications

Phosphorylated by DYRK1A (By similarity). In platelets, phosphorylated in response to thrombin, phorbol-12-myristate-13-acetate and collagen.

Subcellular localisation

Cytoskeleton

Product protocols

Target data

Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion (By similarity).
See full target information SEPTIN5

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