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Recombinant Human Septin 2 protein is a Human Full Length protein, in the 1 to 361 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.

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Images

SDS-PAGE - Recombinant Human Septin 2 protein (AB99296), expandable thumbnail

Publications

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, MS

Biologically active

No

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M S K Q Q P T Q F I N P E T P G Y V G F A N L P N Q V H R K S V K K G F E F T L M V V G E S G L G K S T L I N S L F L T D L Y P E R V I S G A A E K I E R T V Q I E A S T V E I E E R G V K L R L T V V D T P G Y G D A I N C R D C F K T I I S Y I D E Q F E R Y L H D E S G L N R R H I I D N R V H C C F Y F I S P F G H G L K P L D V A F M K A I H N K V N I V P V I A K A D T L T L K E R E R L K K R I L D E I E E H N I K I Y H L P D A E S D E D E D F K E Q T R L L K A S I P F S V V G S N Q L I E A K G K K V R G R L Y P W G V V E V E N P E H N D F L K L R T M L I T H M Q D L Q E V T Q D L H Y E N F R S E R L K R G G R K V E N E D M N K D Q I L L E K E A E L R R M Q E M I A R M Q A Q M Q M Q M Q G G D G D G G A L G H H V

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Filament-forming cytoskeletal GTPase. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed:25588830). Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

Alternative names

Recommended products

Recombinant Human Septin 2 protein is a Human Full Length protein, in the 1 to 361 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, MS

Accession
Q15019-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Sequence info

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M S K Q Q P T Q F I N P E T P G Y V G F A N L P N Q V H R K S V K K G F E F T L M V V G E S G L G K S T L I N S L F L T D L Y P E R V I S G A A E K I E R T V Q I E A S T V E I E E R G V K L R L T V V D T P G Y G D A I N C R D C F K T I I S Y I D E Q F E R Y L H D E S G L N R R H I I D N R V H C C F Y F I S P F G H G L K P L D V A F M K A I H N K V N I V P V I A K A D T L T L K E R E R L K K R I L D E I E E H N I K I Y H L P D A E S D E D E D F K E Q T R L L K A S I P F S V V G S N Q L I E A K G K K V R G R L Y P W G V V E V E N P E H N D F L K L R T M L I T H M Q D L Q E V T Q D L H Y E N F R S E R L K R G G R K V E N E D M N K D Q I L L E K E A E L R R M Q E M I A R M Q A Q M Q M Q M Q G G D G D G G A L G H H V

Accession

Q15019

Protein length

Full Length

Predicted molecular weight

43.6 kDa

Amino acids

1 to 361

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

Additional notes

ab99296 is purified using conventional chromatography techniques.

General info

Function

Filament-forming cytoskeletal GTPase. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed:25588830). Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

Sequence similarities

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.

Subcellular localisation

Cytoskeleton, Spindle

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Septin 2 also known as septin-2 or SEPT2 is a GTP-binding protein with a molecular mass of approximately 41 kDa. It functions mechanically as a major component of the cytoskeletal structure playing an essential role in cellular organization and division. Septin 2 is expressed across various human tissues and cell types indicative of its fundamental role in maintaining cellular integrity. It participates in organizing the actin cytoskeleton and is necessary for cytokinesis.

Biological function summary

Septin 2 integrates into a filamentous network interacting closely with other septins to form hetero-oligomeric complexes essential for cellular dynamics. The protein contributes to the formation of diffusion barriers within membranes facilitating compartmentalization in the cells. As a structural element it supports cellular morphogenesis polarity and vesicle trafficking. The collaborative action within septin complexes ensures effective cell cycle progression and signal transduction.

Pathways

Septin 2 plays a part in key cellular pathways including the cell cycle and apoptosis pathways. The interaction of Septin 2 with proteins like Cdc42 and Rac1 impacts signaling cascades pivotal for actin cytoskeleton organization. The septin complex is important for modulating these pathways as it influences cellular responses to environmental cues. Through these pathways Septin 2 contributes to critical processes such as mitotic spindle positioning and stability.

Associated diseases and disorders

Altered expression or function of Septin 2 is linked to cancer including gliomas where septin dysregulation affects tumor growth and metastasis. Additionally the protein has connections to neurodegenerative disorders such as Alzheimer's disease. Within these contexts proteins like Cdc42 and Rac1 further influence Septin 2's role modulating disease progression through pathways involved in cell migration and proliferation. Understanding Septin 2 in these disorders provides insight into potential therapeutic targets for intervention.

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1 product image

  • SDS-PAGE - Recombinant Human Septin 2 protein (ab99296), expandable thumbnail

    SDS-PAGE - Recombinant Human Septin 2 protein (ab99296)

    15% SDS-PAGE showing ab99296 (3μg).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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