Recombinant Human SERPINB1/PI2 protein is a Human Full Length protein, in the 1 to 379 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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- The Journal of clinical investigation 124:4445-582014Proteinase 3-dependent caspase-3 cleavage modulates neutrophil death and inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFabien Loison et. al.PubMed 25180606
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M E Q L S S A N T R F A L D L F L A L S E N N P A G N I F I S P F S I S S A M A M V F L G T R G N T A A Q L S K T F H F N T V E E V H S R F Q S L N A D I N K R G A S Y I L K L A N R L Y G E K T Y N F L P E F L V S T Q K T Y G A D L A S V D F Q H A S E D A R K T I N Q W V K G Q T E G K I P E L L A S G M V D N M T K L V L V N A I Y F K G N W K D K F M K E A T T N A P F R L N K K D R K T V K M M Y Q K K K F A Y G Y I E D L K C R V L E L P Y Q G E E L S M V I L L P D D I E D E S T G L K K I E E Q L T L E K L H E W T K P E N L D F I E V N V S L P R F K L E E S Y T L N S D L A R L G V Q D L F N S S K A D L S G M S G A R D I F I S K I V H K S F V E V N E E G T E A A A A T A G I A T F C M L M P E E N F T A D H P F L F F I R H N S S G S I L F L G R F S S P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis (PubMed:30692621). Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3 (PubMed:11747453, PubMed:30692621). Acts also as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity (PubMed:23269243). During inflammation, limits the activity of inflammatory caspases CASP1, CASP4 and CASP5 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation (PubMed:30692621). When secreted, promotes the proliferation of beta-cells via its protease inhibitory function (PubMed:26701651).
Alternative names
ELANH2, MNEI, PI2, SERPINB1, Leukocyte elastase inhibitor, LEI, Monocyte/neutrophil elastase inhibitor, Peptidase inhibitor 2, Serpin B1, EI, M/NEI, PI-2
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Recombinant Human SERPINB1/PI2 protein is a Human Full Length protein, in the 1 to 379 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P30740-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M E Q L S S A N T R F A L D L F L A L S E N N P A G N I F I S P F S I S S A M A M V F L G T R G N T A A Q L S K T F H F N T V E E V H S R F Q S L N A D I N K R G A S Y I L K L A N R L Y G E K T Y N F L P E F L V S T Q K T Y G A D L A S V D F Q H A S E D A R K T I N Q W V K G Q T E G K I P E L L A S G M V D N M T K L V L V N A I Y F K G N W K D K F M K E A T T N A P F R L N K K D R K T V K M M Y Q K K K F A Y G Y I E D L K C R V L E L P Y Q G E E L S M V I L L P D D I E D E S T G L K K I E E Q L T L E K L H E W T K P E N L D F I E V N V S L P R F K L E E S Y T L N S D L A R L G V Q D L F N S S K A D L S G M S G A R D I F I S K I V H K S F V E V N E E G T E A A A A T A G I A T F C M L M P E E N F T A D H P F L F F I R H N S S G S I L F L G R F S S P
- Accession
- P30740
- Protein length
- Full Length
- Predicted molecular weight
- 67.43 kDa
- Amino acids
- 1 to 379
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis (PubMed:30692621). Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3 (PubMed:11747453, PubMed:30692621). Acts also as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity (PubMed:23269243). During inflammation, limits the activity of inflammatory caspases CASP1, CASP4 and CASP5 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation (PubMed:30692621). When secreted, promotes the proliferation of beta-cells via its protease inhibitory function (PubMed:26701651).
- Sequence similarities
Belongs to the serpin family. Ov-serpin subfamily.
- Subcellular localisation
- Cytolytic granule, Early endosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as SERPINB1.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SERPINB1 also known as PI2 functions as a serine protease inhibitor. It has an approximate mass of 42 kDa. Serine protease inhibitors play a role in regulating a variety of proteases to maintain cellular homeostasis. This protein mainly expresses in neutrophils epithelial cells and certain immune cells. By inhibiting neutrophil elastase and cathepsin G SERPINB1 protects tissues from protease-driven damage during inflammatory responses.
- Biological function summary
SERPINB1 acts to control proteolytic activity and ensures regulated inflammatory responses. It does not form any known complexes but interacts directly with other proteases to modulate their activity. By neutralizing serine proteases SERPINB1 helps in maintaining the balance in immune cell functions. This regulation contributes to the prevention of excessive tissue damage during inflammation emphasizing its role in immune homeostasis.
- Pathways
SERPINB1 participates in key regulatory processes such as immune response and protease inhibition pathways. It has a significant influence on pathways involving serine proteases like neutrophil elastase pathways. By interacting with these proteases SERPINB1 modulates their activities ensuring controlled inflammatory reactions and protection against tissue damage.
- Associated diseases and disorders
SERPINB1 links to conditions like chronic obstructive pulmonary disease (COPD) and acute lung injury. These conditions often involve excessive proteolytic activity and SERPINB1 acts to counterbalance this to protect the lung tissue. Additionally the protein shows interactions with other proteins like alpha-1-antitrypsin which also acts as a serine protease inhibitor. The relationship between these proteins highlights their collective role in managing protease activity associated with lung diseases.
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1 product image
SDS-PAGE - Recombinant Human SERPINB1/PI2 protein (ab114774)
ab114774 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.
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