Recombinant Human SerpinB3/SCCA protein is a Human Full Length protein, in the 1 to 390 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M N S L S E A N T K F M F D L F Q Q F R K S K E N N I F Y S P I S I T S A L G M V L L G A K D N T A Q Q I K K V L H F D Q V T E N T T G K A A T Y H V D R S G N V H H Q F Q K L L T E F N K S T D A Y E L K I A N K L F G E K T Y L F L Q E Y L D A I K K F Y Q T S E S V D F A N A P E E S R K K I N S W V E S Q T N E K I K N L I P E G N I G S N T T L V L N A I Y F K G Q W E K K F N K E D T K E E K F W P N K N T Y K S I Q M M R Q Y T S F H F A S L E D V Q A K V L E I P Y K G K D L S M I V L L P N E I D G L Q K L E E K L T A E K L M E W T S L Q N M R E T R V D L H L P R F K V E E S Y D L K D T L R T M G M V D I F N G D A D L S G M T G S R G L V L S G V L H K A F V E V T E E G A E A A A A T A V V G F G S S P T S T N E E F H C N H P F L F F I R Q N K T N S I L F Y G R F S S P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May act as a papain-like cysteine protease inhibitor to modulate the host immune response against tumor cells. Also functions as an inhibitor of UV-induced apoptosis via suppression of the activity of c-Jun NH(2)-terminal kinase (JNK1).
Alternative names
SCCA, SCCA1, SERPINB3, Serpin B3, Protein T4-A, Squamous cell carcinoma antigen 1, SCCA-1
Recommended products
Recombinant Human SerpinB3/SCCA protein is a Human Full Length protein, in the 1 to 390 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P29508-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 89% PBS, 10% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M N S L S E A N T K F M F D L F Q Q F R K S K E N N I F Y S P I S I T S A L G M V L L G A K D N T A Q Q I K K V L H F D Q V T E N T T G K A A T Y H V D R S G N V H H Q F Q K L L T E F N K S T D A Y E L K I A N K L F G E K T Y L F L Q E Y L D A I K K F Y Q T S E S V D F A N A P E E S R K K I N S W V E S Q T N E K I K N L I P E G N I G S N T T L V L N A I Y F K G Q W E K K F N K E D T K E E K F W P N K N T Y K S I Q M M R Q Y T S F H F A S L E D V Q A K V L E I P Y K G K D L S M I V L L P N E I D G L Q K L E E K L T A E K L M E W T S L Q N M R E T R V D L H L P R F K V E E S Y D L K D T L R T M G M V D I F N G D A D L S G M T G S R G L V L S G V L H K A F V E V T E E G A E A A A A T A V V G F G S S P T S T N E E F H C N H P F L F F I R Q N K T N S I L F Y G R F S S P
- Accession
- P29508
- Protein length
- Full Length
- Predicted molecular weight
- 47 kDa
- Amino acids
- 1 to 390
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab202159 was purified by using conventional chromatography techniques.
General info
- Function
May act as a papain-like cysteine protease inhibitor to modulate the host immune response against tumor cells. Also functions as an inhibitor of UV-induced apoptosis via suppression of the activity of c-Jun NH(2)-terminal kinase (JNK1).
- Sequence similarities
Belongs to the serpin family. Ov-serpin subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Protein previously labeled as SerpinB3.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SerpinB3 also known as Squamous Cell Carcinoma Antigen (SCCA) is part of the serine protease inhibitor family. This protein has a molecular mass of approximately 45 kDa. It is expressed in various human tissues including skin liver and the respiratory epithelium. SerpinB3 acts by inhibiting cysteine proteinases through binding to them which helps regulate their activity and prevent excessive proteolysis. Such regulatory mechanisms are essential for maintaining cellular homeostasis.
- Biological function summary
SerpinB3 plays a role in cellular proliferation differentiation and protection against apoptosis. It is not reported as part of a specific protein complex but it engages in interactions that influence cell signaling pathways. By protecting cells from programmed cell death SerpinB3 can contribute to tissue repair and regeneration. Its expression levels can change significantly during cell stress responses suggesting its involvement in adaptive processes to environmental stimuli.
- Pathways
Protein SerpinB3 is involved in inflammatory and immune pathways. It influences the NF-κB signaling pathway which is important for immune response regulation and inflammation. This pathway involves multiple proteins including IκB which acts as an inhibitor and other cytokines and transcription factors that facilitate signal transduction in response to stress. SerpinB3’s modulation of these pathways reflects how it affects both innate and adaptive immune responses.
- Associated diseases and disorders
SerpinB3 has been associated with the progression of squamous cell carcinoma and liver fibrosis. In squamous cell carcinoma its interaction with proteases like cathepsin contributes to cancer cell survival and metastasis. For liver fibrosis SerpinB3’s inhibition of apoptosis in hepatocytes may lead to excessive tissue scarring. These connections illustrate its potential as a biomarker for diagnosis and as a target for therapeutic intervention.
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1 product image
SDS-PAGE - Recombinant Human SerpinB3/SCCA protein (ab202159)
15% SDS-PAGE analysis of ab202159 (3μg).
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