Recombinant Human SERPINE1 Protein (Active) is a Human Full Length protein, in the 24 to 402 aa range, expressed in HEK 293, with >=95% purity, >= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC, Biological Activity.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- >= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, MS, HPLC, Biological Activity
- Biologically active
- Yes
Amino acid sequence
V H H P P S Y V A H L A S D F G V R V F Q Q V A Q A S K D R N V V F S P Y G V A S V L A M L Q L T T G G E T Q Q Q I Q A A M G F K I D D K G M A P A L R H L Y K E L M G P W N K D E I S T T D A I F V Q R D L K L V Q G F M P H F F R L F R S T V K Q V D F S E V E R A R F I I N D W V K T H T K G M I S N L L G K G A V D Q L T R L V L V N A L Y F N G Q W K T P F P D S S T H R R L F H K S D G S T V S V P M M A Q T N K F N Y T E F T T P D G H Y Y D I L E L P Y H G D T L S M F I A A P Y E K E V P L S A L T N I L S A Q L I S H W K G N M T R L P R L L V L P K F S L E T E V D L R K P L E N L G M T D M F R Q F Q A D F T S L S D Q E P L H V A Q A L Q K V K I E V N E S G T V A S S S T A V I V S A R M A P E E I I M D R P F L F V V R H N P T G T V L F M G Q V M E P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Biological Activity | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Serine protease inhibitor. Inhibits TMPRSS7 (PubMed:15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed:17912461, PubMed:8481516, PubMed:9207454). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed:9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed:15001579, PubMed:9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed:18386027). It is involved in cellular and replicative senescence (PubMed:16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed:25808697, PubMed:27046084).
Alternative names
PAI1, PLANH1, SERPINE1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1
Recommended products
Recombinant Human SERPINE1 Protein (Active) is a Human Full Length protein, in the 24 to 402 aa range, expressed in HEK 293, with >=95% purity, >= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC, Biological Activity.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- >= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, MS, HPLC, Biological Activity
- Biologically active
- Yes
- Biological activity
- Fully active determined by its ability to inhibit PLAU mediated cleavage of a peptide substrate N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). ED50 for this effect is ≤1.57 ug/ml corresponding to specific activity of 6.36x103 units/mg.
- Accession
- P05121-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- V H H P P S Y V A H L A S D F G V R V F Q Q V A Q A S K D R N V V F S P Y G V A S V L A M L Q L T T G G E T Q Q Q I Q A A M G F K I D D K G M A P A L R H L Y K E L M G P W N K D E I S T T D A I F V Q R D L K L V Q G F M P H F F R L F R S T V K Q V D F S E V E R A R F I I N D W V K T H T K G M I S N L L G K G A V D Q L T R L V L V N A L Y F N G Q W K T P F P D S S T H R R L F H K S D G S T V S V P M M A Q T N K F N Y T E F T T P D G H Y Y D I L E L P Y H G D T L S M F I A A P Y E K E V P L S A L T N I L S A Q L I S H W K G N M T R L P R L L V L P K F S L E T E V D L R K P L E N L G M T D M F R Q F Q A D F T S L S D Q E P L H V A Q A L Q K V K I E V N E S G T V A S S S T A V I V S A R M A P E E I I M D R P F L F V V R H N P T G T V L F M G Q V M E P
- Accession
- P05121
- Protein length
- Full Length
- Predicted molecular weight
- 42.83 kDa
- Amino acids
- 24 to 402
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Serine protease inhibitor. Inhibits TMPRSS7 (PubMed:15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed:17912461, PubMed:8481516, PubMed:9207454). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed:9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed:15001579, PubMed:9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed:18386027). It is involved in cellular and replicative senescence (PubMed:16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed:25808697, PubMed:27046084).
- Sequence similarities
Belongs to the serpin family.
- Post-translational modifications
Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Biological Activity - Recombinant Human SERPINE1 Protein (Active) (ab282405)
Fully active determined by its ability to inhibit PLAU mediated cleavage of a peptide substrate N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). ED50 for this effect is ≤1.57 ug/ml corresponding to specific activity of 6.36x103 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3396380-2
SDS-PAGE - Recombinant Human SERPINE1 Protein (Active) (ab282405)
SDS-page analysis of ab282405
Mass Spectrometry - Recombinant Human SERPINE1 Protein (Active) (ab282405)
Mass determination by ESI-TOF.
Predicted MW is 42826.25 Da (+/- 10 Da by ESI-TOF). Observed MW is 42828.92
HPLC - Recombinant Human SERPINE1 Protein (Active) (ab282405)
HPLC analysis of ab282405.
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