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AB282405

Recombinant Human SERPINE1 Protein (Active)

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Recombinant Human SERPINE1 Protein (Active) is a Human Full Length protein, in the 24 to 402 aa range, expressed in HEK 293 cells, with >95%, >0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, HPLC, Biological Activity, FuncS.

View Alternative Names

PAI1, PLANH1, SERPINE1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1

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Mass Spectrometry - Recombinant Human SERPINE1 Protein (Active) (AB282405)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human SERPINE1 Protein (Active) (AB282405)

Mass determination by ESI-TOF.

Predicted MW is 42826.25 Da (+/- 10 Da by ESI-TOF). Observed MW is 42828.92

Biological Activity - Recombinant Human SERPINE1 Protein (Active) (AB282405)
  • Biological Activity

Unknown

Biological Activity - Recombinant Human SERPINE1 Protein (Active) (AB282405)

Fully active determined by its ability to inhibit PLAU mediated cleavage of a peptide substrate N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). ED50 for this effect is ≤1.57 ug/ml corresponding to specific activity of 6.36x103 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot GR3396380-2

HPLC - Recombinant Human SERPINE1 Protein (Active) (AB282405)
  • HPLC

Supplier Data

HPLC - Recombinant Human SERPINE1 Protein (Active) (AB282405)

HPLC analysis of ab282405.

SDS-PAGE - Recombinant Human SERPINE1 Protein (Active) (AB282405)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human SERPINE1 Protein (Active) (AB282405)

SDS-page analysis of ab282405

Key facts

Purity

>95% HPLC

Endotoxin level

>0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Biological Activity, SDS-PAGE, HPLC, FuncS, Mass Spec

applications

Biologically active

Yes

Biological activity

Fully active determined by its ability to inhibit PLAU mediated cleavage of a peptide substrate N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). ED50 for this effect is ≤1.57 ug/ml corresponding to specific activity of 6.36x103 units/mg.

Accession

P05121

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Complementary Products

Primary Antibodies

AB66705

Anti-PAI1 antibody

BOND RX™ Validated

Western blot - Anti-PAI1 antibody (AB66705)

  • 4
  • 8
Assay Kits

AB242296

Gamma H2A.X Staining Kit

Fluorescence Microscopy - Gamma H2A.X Staining Kit (AB242296)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB217407

Recombinant human Vitronectin/S-Protein (Animal Free)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Biological Activity": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }
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Sequence info

[{"sequence":"VHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAVDQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPKFSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASSSTAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP","proteinLength":"Full Length","predictedMolecularWeight":"42.83 kDa","actualMolecularWeight":null,"aminoAcidEnd":402,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P05121","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

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General info

Function

Serine protease inhibitor. Inhibits TMPRSS7 (PubMed : 15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed : 17912461, PubMed : 8481516, PubMed : 9207454, PubMed : 21925150). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed : 9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed : 15001579, PubMed : 9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed : 18386027). It is involved in cellular and replicative senescence (PubMed : 16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed : 25808697, PubMed : 27046084).

Sequence similarities

Belongs to the serpin family.

Post-translational modifications

Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond.

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Product protocols

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Target data

Serine protease inhibitor. Inhibits TMPRSS7 (PubMed : 15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed : 17912461, PubMed : 8481516, PubMed : 9207454, PubMed : 21925150). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed : 9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed : 15001579, PubMed : 9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed : 18386027). It is involved in cellular and replicative senescence (PubMed : 16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed : 25808697, PubMed : 27046084).
See full target information SERPINE1
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