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AB176039

Recombinant human SERPING1 protein

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Recombinant human SERPING1 protein is a Human Fragment protein, in the 23 to 500 aa range, expressed in CHO cells, with >95%, < 1 EU/µg endotoxin level, suitable for HPLC, SDS-PAGE, FuncS.

View Alternative Names

C1IN, C1NH, SERPING1, Plasma protease C1 inhibitor, C1 Inh, C1Inh, C1 esterase inhibitor, C1-inhibiting factor, Serpin G1

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

CHO cells

Tags

Tag free

Applications

HPLC, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Measured by its ability to inhibit recombinant human complement component C1a cleavage of a colorimetric peptide substrate, N Carbobenzyloxy-Lys- ThioBenzyl ester (Z-K-SBzl). The expected IC50 is ≤ 2.6 nM.

Accession

P05155

Animal free

No

Carrier free

Yes

Species

Human

Storage buffer

Constituents: 0.14% Sodium phosphate

storage-buffer

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Complementary Products

Proteins & Peptides

AB229370

Recombinant human Serpin A5 protein (Active)

SDS-PAGE - Recombinant human Serpin A5 protein (Active) (AB229370)

  • 0
  • 0
Primary Antibodies

AB134918

Anti-SERPING1 antibody [EPR8015]

RabMAb|Recombinant

Western blot - Anti-SERPING1 antibody [EPR8015] (AB134918)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Assay Kits

AB224883

Human C1 inhibitor ELISA Kit

Recombinant|SimpleStep

ELISA - Human C1 inhibitor ELISA Kit (AB224883)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Migrates at an apparent molecular weight of approximately 80-90 kDa by SDS PAGE analysis under reducing conditions.</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as C1 Inactivator
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Sequence info

[{"sequence":"NPNATSSSSQDPESLQDRGEGKVATTVISKMLFVEPILEVSSLPTTNSTTNSATKITANTTDEPTTQPTTEPTTQPTIQPTQPTTQLPTDSPTQPTTGSFCPGPVTLCSDLESHSTEAVLGDALVDFSLKLYHAFSAMKKVETNMAFSPFSIASLLTQVLLGAGENTKTNLESILSYPKDFTCVHQALKGFTTKGVTSVSQIFHSPDLAIRDTFVNASRTLYSSSPRVLSNNSDANLELINTWVAKNTNNKISRLLDSLPSDTRLVLLNAIYLSAKWKTTFDPKKTRMEPFHFKNSVIKVPMMNSKKYPVAHFIDQTLKAKVGQLQLSHNLSLVILVPQNLKHRLEDMEQALSPSVFKAIMEKLEMSKFQPTLLTLPRIKVTTSQDMLSIMEKLEFFDFSYDLNLCGLTEDPDLQVSAMQHQTVLELTETGVEAAAASAISVARTLLVFEVQQPFLFVLWDQQHKFPVFMGRVYDPRA","proteinLength":"Fragment","predictedMolecularWeight":"52.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":500,"aminoAcidStart":23,"nature":"Recombinant","expressionSystem":"CHO cells","accessionNumber":"P05155","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
A few weeks
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon reconstitution add a carrier protein (0.1% BSA)
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SERPING1 also known as C1 inhibitor or C1-inactivator is a protein that plays an important mechanical role in the regulation of the complement system. It is a member of the serpin superfamily and has a molecular mass of approximately 105-110 kDa. This protein is mainly expressed in the liver but also present in other tissues such as monocytes and epithelial cells. As an inhibitor SERPING1 effectively regulates the activity of the complement subcomponents C1r and C1s plasmin and kallikrein by inhibiting their protease activity preventing unwanted activation of the complement cascade.
Biological function summary

The protein acts as a critical control point in the complement and contact activation system. It is part of the C1 complex which includes the proteins C1q C1r and C1s. The regulation extends to pathways that involve the breakdown of plasma proteins and prevention of spontaneous inflammatory reactions. By controlling these pathways the protein prevents excessive inflammation and tissue damage which can occur during immune responses. Its involvement extends to the regulation of the complement system and maintenance of vascular permeability.

Pathways

SERPING1 interacts closely with mechanisms involved in the complement cascade and the kinin-kallikrein pathways. It associates with proteins like MASP-1 and MASP-2 important in the complement and lectin pathways. These pathways are important for innate immunity and influence factors like inflammation and blood pressure. SERPING1 modulates these pathways by inhibiting key enzymes and ensuring controlled responses therefore maintaining systemic balance within the immune system.

The malfunction or deficiency of SERPING1 has direct links to hereditary angioedema a condition marked by severe and rapid swelling episodes. The disorder results from inadequate inhibition of bradykinin emphasizing the protein's role in vascular regulation. Additionally the protein has implications in autoimmune diseases such as systemic lupus erythematosus where complement regulation is disrupted. The interplay between SERPING1 and other proteins such as bradykinin receptor B2 remains significant in understanding disease mechanisms and developing therapeutic interventions.
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Specifications

Form

Lyophilized

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General info

Function

Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein.

Sequence similarities

Belongs to the serpin family.

Post-translational modifications

Highly glycosylated (49%) with N- and O-glycosylation. O-glycosylated with core 1 or possibly core 8 glycans. N-glycan heterogeneity at Asn-25: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor).. Can be proteolytically cleaved by E.coli stcE.

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Product protocols

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Target data

Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein.
See full target information SERPING1
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Product promise

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