Recombinant Human Serum Albumin protein (His tag)
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(2 Publications)
Recombinant Human Serum Albumin protein (His tag) is a Human Full Length protein, in the 25 to 609 aa range with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE and Functional studies. The predicted molecular weight of ab217817 protein is 67.3 kDa.
- Save time and ensure accurate results - use our Serum Albumin protein as a control
- Optimal protein bioactivity, stability and reproducibility
View Alternative Names
GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin
- Biological Activity
Supplier Data
Biological Activity - Recombinant Human Serum Albumin protein (His tag) (AB217817)
Human Serum Albumin Protein, His Tag (ab217817) immobilized on CM4 Chip can bind Human FCGRT&B2M Heterodimer Protein, His Tag with an affinity constant of 0.381 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
- FuncS
Supplier Data
Functional Studies - Recombinant Human Serum Albumin protein (His tag) (AB217817)
Bioactivity SPR : Immobilized biotinyated Huamn FcRN Heterodimer protein on SA chip can bind ab217817, his tag with an affitinity constant of 1.78μM.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human Serum Albumin protein (His tag) (AB217817)
SDS-PAGE analysis of DTT-reduced ab217817 followed by staining overnight with Coomassie Blue.
DTT-reduced protein migrates as 66 kDa in SDS-PAGE due to glycosylation.
- Size Exclusion Chromatography
Supplier Data
Size Exclusion Chromatography - Recombinant Human Serum Albumin protein (His tag) (AB217817)
The purity of Human Serum Albumin, His Tag (ab217817) is more than 90% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.
Reactivity data
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Why is this recommended?
We recommend this product because it’s often used in the same experiment or related research.
We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.
Product details
Check out our protein gel staining guide for SDS-PAGE here
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Specifications
Form
Lyophilized
Additional notes
>95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
General info
Function
Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed : 19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Post-translational modifications
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.. Glycated in diabetic patients.. Phosphorylated by FAM20C in the extracellular medium.. Acetylated on Lys-223 by acetylsalicylic acid.
Product protocols
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Target data
Publications (2)
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The Journal of biological chemistry 299:104998 PubMed37394009
2023
Applications
Unspecified application
Species
Unspecified reactive species
Microbiology spectrum 10:e0269721 PubMed35080440
2022
Applications
Unspecified application
Species
Unspecified reactive species
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