Recombinant Human Serum Albumin protein (His tag) is a Human Full Length protein, in the 25 to 609 aa range with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE and Functional studies. The predicted molecular weight of ab217817 protein is 67.3 kDa.
- Save time and ensure accurate results - use our Serum Albumin protein as a control
- Optimal protein bioactivity, stability and reproducibility
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Publications
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- Microbiology spectrum 10:e02697212022The Linker Region Promotes Activity and Binding Efficiency of Modular LPMO towards Polymeric Substrate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAishwarya Srivastava,Pragya Nagar,Sumit Rathore,Nidhi AdlakhaPubMed 35080440
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
D A H K S E V A H R F K D L G E E N F K A L V L I A F A Q Y L Q Q C P F E D H V K L V N E V T E F A K T C V A D E S A E N C D K S L H T L F G D K L C T V A T L R E T Y G E M A D C C A K Q E P E R N E C F L Q H K D D N P N L P R L V R P E V D V M C T A F H D N E E T F L K K Y L Y E I A R R H P Y F Y A P E L L F F A K R Y K A A F T E C C Q A A D K A A C L L P K L D E L R D E G K A S S A K Q R L K C A S L Q K F G E R A F K A W A V A R L S Q R F P K A E F A E V S K L V T D L T K V H T E C C H G D L L E C A D D R A D L A K Y I C E N Q D S I S S K L K E C C E K P L L E K S H C I A E V E N D E M P A D L P S L A A D F V E S K D V C K N Y A E A K D V F L G M F L Y E Y A R R H P D Y S V V L L L R L A K T Y E T T L E K C C A A A D P H E C Y A K V F D E F K P L V E E P Q N L I K Q N C E L F E Q L G E Y K F Q N A L L V R Y T K K V P Q V S T P T L V E V S R N L G K V G S K C C K H P E A K R M P C A E D Y L S V V L N Q L C V L H E K T P V S D R V T K C C T E S L V N R R P C F S A L E V D E T Y V P K E F N A E T F T F H A D I C T L S E K E R Q I K K Q T A L V E L V K H K P K A T K E Q L K A V M D D F A A F V E K C C K A D D K E T C F A E E G K K L V A A S Q A A L G L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).
Alternative names
GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin
Recommended products
Recombinant Human Serum Albumin protein (His tag) is a Human Full Length protein, in the 25 to 609 aa range with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE and Functional studies. The predicted molecular weight of ab217817 protein is 67.3 kDa.
- Save time and ensure accurate results - use our Serum Albumin protein as a control
- Optimal protein bioactivity, stability and reproducibility
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- ab217817 immobilized on CM4 Chip can bind Human FCGRT&B2M Heterodimer Protein with an affinity constant of 0.381 μM as determined in a SPR assay.
- Accession
- P02768-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 200 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 90% PBS, 10% Trehalose
Sequence info
Amino acid sequence
- D A H K S E V A H R F K D L G E E N F K A L V L I A F A Q Y L Q Q C P F E D H V K L V N E V T E F A K T C V A D E S A E N C D K S L H T L F G D K L C T V A T L R E T Y G E M A D C C A K Q E P E R N E C F L Q H K D D N P N L P R L V R P E V D V M C T A F H D N E E T F L K K Y L Y E I A R R H P Y F Y A P E L L F F A K R Y K A A F T E C C Q A A D K A A C L L P K L D E L R D E G K A S S A K Q R L K C A S L Q K F G E R A F K A W A V A R L S Q R F P K A E F A E V S K L V T D L T K V H T E C C H G D L L E C A D D R A D L A K Y I C E N Q D S I S S K L K E C C E K P L L E K S H C I A E V E N D E M P A D L P S L A A D F V E S K D V C K N Y A E A K D V F L G M F L Y E Y A R R H P D Y S V V L L L R L A K T Y E T T L E K C C A A A D P H E C Y A K V F D E F K P L V E E P Q N L I K Q N C E L F E Q L G E Y K F Q N A L L V R Y T K K V P Q V S T P T L V E V S R N L G K V G S K C C K H P E A K R M P C A E D Y L S V V L N Q L C V L H E K T P V S D R V T K C C T E S L V N R R P C F S A L E V D E T Y V P K E F N A E T F T F H A D I C T L S E K E R Q I K K Q T A L V E L V K H K P K A T K E Q L K A V M D D F A A F V E K C C K A D D K E T C F A E E G K K L V A A S Q A A L G L
- Accession
- P02768
- Protein length
- Full Length
- Predicted molecular weight
- 67.3 kDa
- Amino acids
- 25 to 609
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
>95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
General info
- Function
Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).
- Sequence similarities
Belongs to the ALB/AFP/VDB family.
- Post-translational modifications
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C, -80°C
- Appropriate long-term storage conditions
- -20°C, -80°C
- Storage information
- Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Ensure the validity of your result using our bioactive recombinant human Serum Albumin protein (His tag) ab217817 as a positive control in SDS-PAGE. The rHSA ab217817 molecular weight is 67.3 kDa.
Check out our protein gel staining guide for SDS-PAGE here
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
SDS-PAGE - Recombinant Human Serum Albumin protein (His tag) (ab217817)
SDS-PAGE analysis of DTT-reduced ab217817 followed by staining overnight with Coomassie Blue.
DTT-reduced protein migrates as 66 kDa in SDS-PAGE due to glycosylation.
Size Exclusion Chromatography - Recombinant Human Serum Albumin protein (His tag) (ab217817)
The purity of Human Serum Albumin, His Tag (ab217817) is more than 90% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.
Biological Activity - Recombinant Human Serum Albumin protein (His tag) (ab217817)
Human Serum Albumin Protein, His Tag (ab217817) immobilized on CM4 Chip can bind Human FCGRT&B2M Heterodimer Protein, His Tag with an affinity constant of 0.381 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Functional Studies - Recombinant Human Serum Albumin protein (His tag) (ab217817)
Bioactivity SPR: Immobilized biotinyated Huamn FcRN Heterodimer protein on SA chip can bind ab217817, his tag with an affitinity constant of 1.78μM.
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