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AB50232

Recombinant human Serum Amyloid A (mutated D60N + H71R) protein

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(1 Publication)

Recombinant human Serum Amyloid A (mutated D60N + H71R) protein is a Human Full Length protein, expressed in Escherichia coli, with >98%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

Serum amyloid A-1 protein, SAA, SAA1

1 Images
Western blot - Recombinant human Serum Amyloid A (mutated D60N + H71R) protein (AB50232)
  • WB

Unknown

Western blot - Recombinant human Serum Amyloid A (mutated D60N + H71R) protein (AB50232)

All lanes:

Western blot - Anti-Serum Amyloid A antibody [115] (<a href='/en-us/products/primary-antibodies/serum-amyloid-a-antibody-115-ab687'>ab687</a>) at 0.5 µg/mL

All lanes:

Western blot - Recombinant human Serum Amyloid A (mutated D60N + H71R) protein (ab50232) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

true

Exposure time: 1min

Key facts

Purity

>98% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, WB, FuncS

applications

Biologically active

Yes

Biological activity

Tested by its ability to down- regulate lipid biosynthesis in aortic smooth muscle cells. The effective concentration was found to be 4 μM. Schreiber, BM. et al. Biochem J. 1999 Nov 15; 344 Pt 1:7-13

Accession

P0DJI8

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Please reconstitute this product in water to a concentration of 1mg/ml.

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Complementary Products

Primary Antibodies

AB190802

Anti-Serum Amyloid A antibody [EP11592-92]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid A antibody [EP11592-92] (AB190802)

  • 0
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB215090

Mouse SAA ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse SAA ELISA Kit (AB215090)

  • 0
  • 0
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab50232 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/serum-amyloid-a-antibody-115-ab687'>ab687</a>.</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>For most in vitro applications, apo-SAA exerts its biological activity in the concentration range of 10-50 µg/ml.</p>" } } }
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Product details

For most in vitro applications, apo-SAA exerts its biological activity in the concentration range of 10-50 μg/ml. ab50232 can be used as a WB positive control in conjunction with ab687.
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Sequence info

[{"sequence":"MRSFFSFLGEAFDGARDMWRAYSDMREANYIGSDKYFHARGNYDAAKRGPGGVWAAEAISNARENIQRFFGRGAEDSLADQAANEWGRSGKDPNHFRPAGLPEKY","proteinLength":"Full Length","predictedMolecularWeight":"11.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P0DJI8","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serum Amyloid A (SAA) is an important acute-phase protein with a mass of approximately 12 kDa. It plays a role in inflammation and is present in the serum. SAA is an apolipoprotein that associates with high-density lipoprotein (HDL) during inflammatory states. The protein is expressed mainly in the liver and circulating SAA levels greatly increase during inflammatory responses marking it as an important marker for inflammation.
Biological function summary

SAA contributes to the body's immune response to injury by recruiting immune cells and facilitating the removal of cholesterol. As part of the acute-phase response SAA integrates into HDL displacing other apolipoproteins and altering HDL's function. Increased SAA levels can also modulate prostaglandin synthesis and secretion influencing the inflammatory response. The exact role of SAA in pathways beyond these functions continues to be a subject of research.

Pathways

SAA influences lipid metabolism and inflammatory pathways. It plays a significant role within the acute-phase response pathway which modulates the immune system’s reaction to stress such as infection or injury. Another pathway involves cholesterol metabolism where SAA interacts with apolipoproteins AI and AII altering the composition and function of HDL particles.

High levels of SAA associate with amyloidosis and chronic inflammatory diseases. In amyloidosis misfolded SAA proteins deposit as amyloid fibrils in tissues leading to organ dysfunction. In dogs and other species SAA is used as a biomarker to monitor inflammatory diseases. Its connection with C-reactive protein further emphasizes its role in inflammatory processes. Research continues to explore how modulating SAA levels may impact disease progression and treatment.
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Specifications

Form

Lyophilized

Additional notes

>=98% by SDS-PAGE and HPLC analyses.

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General info

Function

Major acute phase protein.

Sequence similarities

Belongs to the SAA family.

Post-translational modifications

This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.

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Product protocols

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Target data

Major acute phase protein.
See full target information SAA1 mutated D60N + H71R
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell research 33:585-603 PubMed37337030

2023

An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte-tumor cell crosstalk, local immunosuppression and tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Wu,Jiayan Yan,Yinqi Bai,Feiyu Chen,Xuanxuan Zou,Jiangshan Xu,Ao Huang,Liangzhen Hou,Yu Zhong,Zehua Jing,Qichao Yu,Xiaorui Zhou,Zhifeng Jiang,Chunqing Wang,Mengnan Cheng,Yuan Ji,Yingyong Hou,Rongkui Luo,Qinqin Li,Liang Wu,Jianwen Cheng,Pengxiang Wang,Dezhen Guo,Waidong Huang,Junjie Lei,Shang Liu,Yizhen Yan,Yiling Chen,Sha Liao,Yuxiang Li,Haixiang Sun,Na Yao,Xiangyu Zhang,Shiyu Zhang,Xi Chen,Yang Yu,Yao Li,Fengming Liu,Zheng Wang,Shaolai Zhou,Huanming Yang,Shuang Yang,Xun Xu,Longqi Liu,Qiang Gao,Zhaoyou Tang,Xiangdong Wang,Jian Wang,Jia Fan,Shiping Liu,Xinrong Yang,Ao Chen,Jian Zhou
View all publications
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Product promise

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