Recombinant human Serum Amyloid A (mutated D60N + H71R) protein is a Human Full Length protein, expressed in Escherichia coli, with >=98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, WB, FuncS.
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- Cell research 33:585-6032023An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte-tumor cell crosstalk, local immunosuppression and tumor progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Wu,Jiayan Yan,Yinqi Bai,Feiyu Chen,Xuanxuan Zou,Jiangshan Xu,Ao Huang,Liangzhen Hou,Yu Zhong,Zehua Jing,Qichao Yu,Xiaorui Zhou,Zhifeng Jiang,Chunqing Wang,Mengnan Cheng,Yuan Ji,Yingyong Hou,Rongkui Luo,Qinqin Li,Liang Wu,Jianwen Cheng,Pengxiang Wang,Dezhen Guo,Waidong Huang,Junjie Lei,Shang Liu,Yizhen Yan,Yiling Chen,Sha Liao,Yuxiang Li,Haixiang Sun,Na Yao,Xiangyu Zhang,Shiyu Zhang,Xi Chen,Yang Yu,Yao Li,Fengming Liu,Zheng Wang,Shaolai Zhou,Huanming Yang,Shuang Yang,Xun Xu,Longqi Liu,Qiang Gao,Zhaoyou Tang,Xiangdong Wang,Jian Wang,Jia Fan,Shiping Liu,Xinrong Yang,Ao Chen,Jian ZhouPubMed 37337030
Key facts
- Purity
- >=98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
M R S F F S F L G E A F D G A R D M W R A Y S D M R E A N Y I G S D K Y F H A R G N Y D A A K R G P G G V W A A E A I S N A R E N I Q R F F G R G A E D S L A D Q A A N E W G R S G K D P N H F R P A G L P E K Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab50232 can be used as a WB positive control in conjunction with Anti-Serum Amyloid A antibody [115] ab687. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes For most in vitro applications, apo-SAA exerts its biological activity in the concentration range of 10-50 µg/ml. |
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Target data
Function
Major acute phase protein.
Alternative names
Serum amyloid A-1 protein, SAA, SAA1
Recommended products
Recombinant human Serum Amyloid A (mutated D60N + H71R) protein is a Human Full Length protein, expressed in Escherichia coli, with >=98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >=98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- Tested by its ability to down- regulate lipid biosynthesis in aortic smooth muscle cells. The effective concentration was found to be 4 μM. Schreiber, BM. et al. Biochem J. 1999 Nov 15; 344 Pt 1:7-13
- Accession
- P0DJI8
- Animal free
- No
- Species
- Human
- Reconstitution
- Please reconstitute this product in water to a concentration of 1mg/ml.
- Concentration
Sequence info
Amino acid sequence
- M R S F F S F L G E A F D G A R D M W R A Y S D M R E A N Y I G S D K Y F H A R G N Y D A A K R G P G G V W A A E A I S N A R E N I Q R F F G R G A E D S L A D Q A A N E W G R S G K D P N H F R P A G L P E K Y
- Accession
- P0DJI8
- Protein length
- Full Length
- Predicted molecular weight
- 11.7 kDa
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
>=98% by SDS-PAGE and HPLC analyses.
General info
- Function
Major acute phase protein.
- Sequence similarities
Belongs to the SAA family.
- Post-translational modifications
This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
For most in vitro applications, apo-SAA exerts its biological activity in the concentration range of 10-50 μg/ml. ab50232 can be used as a WB positive control in conjunction with Anti-Serum Amyloid A antibody [115] ab687.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Serum Amyloid A (SAA) is an important acute-phase protein with a mass of approximately 12 kDa. It plays a role in inflammation and is present in the serum. SAA is an apolipoprotein that associates with high-density lipoprotein (HDL) during inflammatory states. The protein is expressed mainly in the liver and circulating SAA levels greatly increase during inflammatory responses marking it as an important marker for inflammation.
- Biological function summary
SAA contributes to the body's immune response to injury by recruiting immune cells and facilitating the removal of cholesterol. As part of the acute-phase response SAA integrates into HDL displacing other apolipoproteins and altering HDL's function. Increased SAA levels can also modulate prostaglandin synthesis and secretion influencing the inflammatory response. The exact role of SAA in pathways beyond these functions continues to be a subject of research.
- Pathways
SAA influences lipid metabolism and inflammatory pathways. It plays a significant role within the acute-phase response pathway which modulates the immune system’s reaction to stress such as infection or injury. Another pathway involves cholesterol metabolism where SAA interacts with apolipoproteins AI and AII altering the composition and function of HDL particles.
- Associated diseases and disorders
High levels of SAA associate with amyloidosis and chronic inflammatory diseases. In amyloidosis misfolded SAA proteins deposit as amyloid fibrils in tissues leading to organ dysfunction. In dogs and other species SAA is used as a biomarker to monitor inflammatory diseases. Its connection with C-reactive protein further emphasizes its role in inflammatory processes. Research continues to explore how modulating SAA levels may impact disease progression and treatment.
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1 product image
Western blot - Recombinant human Serum Amyloid A (mutated D60N + H71R) protein (ab50232)
All lanes: Western blot - Anti-Serum Amyloid A antibody [115] (Anti-Serum Amyloid A antibody [115] ab687) at 0.5 µg/mL
All lanes: Western blot - Recombinant human Serum Amyloid A (mutated D60N + H71R) protein (ab50232) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1min
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