Recombinant Human Serum Amyloid P/SAP protein (His tag) is a Human Full Length protein, in the 1 to 223 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M N K P L L W I S V L T S L L E A F A H T D L S G K V F V F P R E S V T D H V N L I T P L E K P L Q N F T L C F R A Y S D L S R A Y S L F S Y N T Q G R D N E L L V Y K E R V G E Y S L Y I G R H K V T S K V I E K F P A P V H I C V S W E S S S G I A E F W I N G T P L V K K G L R Q G Y F V E A Q P K I V L G Q E Q D S Y G G K F D R S Q S F V G E I G D L Y M W D S V L P P E N I L S A Y Q G T P L P A N I L D W Q A L N Y E I R G Y V I I K P L V W V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
Alternative names
PTX2, APCS, Serum amyloid P-component, SAP, 9.5S alpha-1-glycoprotein
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Recombinant Human Serum Amyloid P/SAP protein (His tag) is a Human Full Length protein, in the 1 to 223 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P02743-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M N K P L L W I S V L T S L L E A F A H T D L S G K V F V F P R E S V T D H V N L I T P L E K P L Q N F T L C F R A Y S D L S R A Y S L F S Y N T Q G R D N E L L V Y K E R V G E Y S L Y I G R H K V T S K V I E K F P A P V H I C V S W E S S S G I A E F W I N G T P L V K K G L R Q G Y F V E A Q P K I V L G Q E Q D S Y G G K F D R S Q S F V G E I G D L Y M W D S V L P P E N I L S A Y Q G T P L P A N I L D W Q A L N Y E I R G Y V I I K P L V W V
- Accession
- P02743
- Protein length
- Full Length
- Predicted molecular weight
- 24.7 kDa
- Amino acids
- 1 to 223
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
- Sequence similarities
Belongs to the pentraxin family.
- Post-translational modifications
N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148).
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Serum Amyloid P component also known as SAP P-SAP or APCS protein is a pentraxin family member. It has a molecular mass of approximately 25 kDa. SAP expresses mainly in the liver and circulates in blood plasma. The protein consists of five identical subunits which form a homopentameric structure. This structure enables SAP to bind to amyloid fibrils a defining mechanical action of this protein.
- Biological function summary
SAP plays a role in the immune system by recognizing damaged cells and pathogens. It acts as an opsonin marking these cellular targets for phagocytosis. As part of a complex SAP stabilizes extracellular matrix and amyloid deposits. It binds specifically to amyloid fibrils found in various tissues potentially facilitating their removal by immune cells. The protein's binding actions are important for its role in maintaining tissue and fluid homeostasis.
- Pathways
SAP is actively involved in the innate immune response and amyloidogenic pathways. It interacts with the complement system to enhance the clearance of cell debris and amyloid fibrils. The complement system is an important component of innate immunity where SAP can engage with complement proteins like C1q. This interaction suggests that SAP plays roles in modulating both inflammation and tissue repair processes.
- Associated diseases and disorders
SAP has significant connections. SAP is related to systemic amyloidosis where it binds to amyloid deposits affecting multiple organs. Moreover SAP has been implicated in Alzheimer's disease due to its interaction with beta-amyloid protein plaques. In these disorders the involvement of SAP aids researchers in understanding the pathological mechanisms and could open avenues for targeted therapies aimed at modulating SAP functions.
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SDS-PAGE - Recombinant Human Serum Amyloid P/SAP protein (His tag) (ab276564)
SDS-PAGE analysis of ab276564
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