Recombinant Human Serum Response Factor SRF protein is a Human Fragment protein, in the 406 to 508 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
H M M Y P S P H A V M Y A P T S G L G D G S L T V L N A F S Q A P S T M Q V S H S Q V Q E P G G V P Q V F L T A S S G T V Q I P V S A V Q L H Q M A V I G Q Q A G S S S N L T E L Q V V N L D T A H S T K S E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.
Alternative names
Serum response factor, SRF
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Recombinant Human Serum Response Factor SRF protein is a Human Fragment protein, in the 406 to 508 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P11831-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- H M M Y P S P H A V M Y A P T S G L G D G S L T V L N A F S Q A P S T M Q V S H S Q V Q E P G G V P Q V F L T A S S G T V Q I P V S A V Q L H Q M A V I G Q Q A G S S S N L T E L Q V V N L D T A H S T K S E
- Accession
- P11831
- Protein length
- Fragment
- Predicted molecular weight
- 36.96 kDa
- Amino acids
- 406 to 508
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.
- Post-translational modifications
Phosphorylated by PRKDC.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Serum Response Factor (SRF) also known as serum response element-binding protein is a transcription factor with a mass of around 67 kDa. SRF regulates gene expression by binding to the serum response element (SRE) found in the promoter region of target genes. It is widely expressed across multiple tissues including muscle brain and epithelial cells. SRF plays a critical role in various cellular functions due to its ability to control the transcription of immediate-early genes in response to a range of extracellular signals.
- Biological function summary
SRF influences cellular processes such as growth differentiation and migration. SRF often functions as a part of a transcriptional complex interacting with co-factors like the ternary complex factor (TCF) from the ETS domain family of transcription factors. This interaction modulates the expression of genes involved in cytoskeletal organization and cell cycle regulation. By doing so SRF coordinates complex cellular activities that are essential for normal development and response to environmental changes.
- Pathways
SRF plays a significant role in the mitogen-activated protein kinase (MAPK) pathway and the RhoA pathway. In the MAPK pathway SRF acts downstream to mediate the response of cells to growth stimuli working closely with MAPK-regulated kinases. Through the RhoA pathway SRF regulates changes in cytoskeletal dynamics and cell movement with the involvement of proteins such as Rho-associated coiled-coil containing protein kinase (ROCK) and myocardin-related transcription factors (MRTFs).
- Associated diseases and disorders
SRF has a strong connection to cardiovascular diseases and certain types of cancer. Alterations in SRF expression or function can contribute to the development of atherosclerosis and myocardial hypertrophy. Additionally dysregulation in SRF activity has been observed in some cancers where it associates with aberrant cell proliferation and migration. In these contexts proteins such as connective tissue growth factor (CTGF) can interact with SRF further impacting disease progression and severity.
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1 product image
SDS-PAGE - Recombinant Human Serum Response Factor SRF protein (ab114319)
SDS-PAGE analysis of ab114319 on a 12.5% gel stained with Coomassie Blue.
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