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AB116194

Recombinant Human Seryl-tRNA synthetase/SERS protein

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Recombinant Human Seryl-tRNA synthetase/SERS protein is a Human Full Length protein, in the 1 to 514 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

SARS, SERS, SARS1, Seryl-tRNA synthetase, Seryl-tRNA(Ser/Sec) synthetase, SerRS

1 Images
SDS-PAGE - Recombinant Human Seryl-tRNA synthetase/SERS protein (AB116194)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Seryl-tRNA synthetase/SERS protein (AB116194)

15% SDS-PAGE showing ab116194 at approximately 61.2kDa (3µg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P49591

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as Seryl-tRNA synthetase
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMVLDLDLFRVDKGGDPALIRETQEKRFKDPGLVDQLVKADSEWRRCRFRADNLNKLKNLCSKTIGEKMKKKEPVGDDESVPENVLSFDDLTADALANLKVSQIKKVRLLIDEAILKCDAERIKLEAERFENLREIGNLLHPSVPISNDEDVDNKVERIWGDCTVRKKYSHVDLVVMVDGFEGEKGAVVAGSRGYFLKGVLVFLEQALIQYALRTLGSRGYIPIYTPFFMRKEVMQEVAQLSQFDEELYKVIGKGSEKSDDNSYDEKYLIATSEQPIAALHRDEWLRPEDLPIKYAGLSTCFRQEVGSHGRDTRGIFRVHQFEKIEQFVYSSPHDNKSWEMFEEMITTAEEFYQSLGIPYHIVNIVSGSLNHAASKKLDLEAWFPGSGAFRELVSCSNCTDYQARRLRIRYGQTKKMMDKVEFVHMLNATMCATTRTICAILENYQTEKGITVPEKLKEFMPPGLQELIPFVKPAPIEQEPSKKQKKQHEGSKKKAAARDVTLENRLQNMEVTDA","proteinLength":"Full Length","predictedMolecularWeight":"61.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":514,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P49591","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Seryl-tRNA synthetase also known as SERS is an enzyme responsible for catalyzing the attachment of serine to its corresponding tRNA molecule a process known as aminoacylation. SERS with a molecular mass of approximately 55 kDa finds its expression in various tissues and cell types where it plays a role in protein synthesis. It operates by ensuring that genetic information is translated accurately from nucleic acid sequences to protein sequences important in maintaining the fidelity of protein biosynthesis.
Biological function summary

Through its function of aminoacylating serine to tRNA SERS contributes to the synthesis of proteins by enabling the incorporation of serine residues into polypeptides. SERS does not act alone; instead it operates as a part of the multi-tRNA synthetase complex enhancing its enzymatic efficiency and aiding in the coordination of protein synthesis. Beyond catalysis SERS also participates in cellular response to stress and has non-canonical roles that extend its function beyond tRNA charging.

Pathways

The role of SERS in translation aligns it with pathways involving protein synthesis and amino acid metabolism. One such pathway is the mTOR signaling pathway which SERS influences indirectly through its role in the availability of serine-loaded tRNAs for protein synthesis. Additionally SERS is linked to serine biosynthesis pathways interconnected with proteins like PHGDH which catalyzes the first step in the serine biosynthesis from 3-phosphoglycerate.

The malfunction or aberrant expression of SERS associates with Charcot-Marie-Tooth disease a neuropathy affecting peripheral nerves. Mutations in SERS can disrupt normal nerve function leading to symptoms associated with this disorder. Furthermore SERS links to cancerous processes as its non-canonical functions may influence tumor progression. In cancer SERS interacts with proteins such as oncogenes or tumor suppressors affecting cellular proliferation and survival.
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Specifications

Form

Liquid

Additional notes

ab116194 was purified using conventional chromatography techniques.

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General info

Function

Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction : serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser) (PubMed : 22353712, PubMed : 24095058, PubMed : 26433229, PubMed : 28236339, PubMed : 34570399, PubMed : 36041817, PubMed : 9431993). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) (PubMed : 26433229, PubMed : 28236339, PubMed : 34570399, PubMed : 9431993). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC (PubMed : 24940000). Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA (PubMed : 19423847, PubMed : 19423848, PubMed : 24940000).

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction : serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser) (PubMed : 22353712, PubMed : 24095058, PubMed : 26433229, PubMed : 28236339, PubMed : 34570399, PubMed : 36041817, PubMed : 9431993). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) (PubMed : 26433229, PubMed : 28236339, PubMed : 34570399, PubMed : 9431993). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC (PubMed : 24940000). Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA (PubMed : 19423847, PubMed : 19423848, PubMed : 24940000).
See full target information SARS1
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