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AB196408

Recombinant Human SESN2/Sestrin-2 protein

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Recombinant Human SESN2/Sestrin-2 protein is a Human Full Length protein, in the 2 to 480 aa range, expressed in Baculovirus infected Sf9 cells, with >35%, suitable for SDS-PAGE.

View Alternative Names

Hi95, SEST2, SESN2, Sestrin-2, Hypoxia-induced gene

1 Images
SDS-PAGE - Recombinant Human SESN2/Sestrin-2 protein (AB196408)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human SESN2/Sestrin-2 protein (AB196408)

4-20% SDS-PAGE analysis of ab196408 at 3 μg stained with Coomassie Blue (lane 1). Lane 2 contains a protein marker.

Key facts

Purity

>35% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

DDDDK tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P58004

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.72% Sodium chloride, 0.71% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling

This product was previously labelled as SESN2

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Sequence info

[{"sequence":"IVADSECRAELKDYLRFAPGGVGDSGPGEEQRESRARRGPRGPSAFIPVEEVLREGAESLEQHLGLEALMSSGRVDNLAVVMGLHPDYFTSFWRLHYLLLHTDGPLASSWRHYIAIMAAARHQCSYLVGSHMAEFLQTGGDPEWLLGLHRAPEKLRKLSEINKLLAHRPWLITKEHIQALLKTGEHTWSLAELIQALVLLTHCHSLSSFVFGCGILPEGDADGSPAPQAPTPPSEQSSPPSRDPLNNSGGFESARDVEALMERMQQLQESLLRDEGTSQEEMESRFELEKSESLLVTPSADILEPSPHPDMLCFVEDPTFGYEDFTRRGAQAPPTFRAQDYTWEDHGYSLIQRLYPEGGQLLDEKFQAAYSLTYNTIAMHSGVDTSVLRRAIWNYIHCVFGIRYDDYDYGEVNQLLERNLKVYIKTVACYPEKTTRRMYNLFWRHFRHSEKVHVNLLLLEARMQAALLYALRAITRYMT","proteinLength":"Full Length","predictedMolecularWeight":"55 kDa","actualMolecularWeight":null,"aminoAcidEnd":480,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P58004","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SESN2 also known as Sestrin-2 or Hi95 is a stress-inducible protein that plays a role in cellular responses to oxidative stress. It has a molecular mass of approximately 60 kDa and is expressed in various tissues including the liver heart and muscle. Sestrin-2 acts as an antioxidant protein that helps reduce reactive oxygen species (ROS) and maintains cellular redox balance. It contains domains that directly interact with target proteins to regulate cellular activity enabling cells to respond efficiently to stress signals.
Biological function summary

Sestrin-2 regulates cell survival and homeostasis during oxidative stress conditions. It modulates the activity of key signaling pathways that control autophagy and cell growth. Sestrin-2 does not function alone but interacts with other proteins to form functional complexes. This allows it to fine-tune cellular processes and contributes to its role in promoting cell repair mechanisms. Its induction during stress conditions demonstrates its engagement in promoting cellular adaptation and resistance to damage.

Pathways

Sestrin-2 engages in signaling networks such as the AMPK and mTOR pathways. Its interaction with AMP-activated protein kinase (AMPK) triggers energy homeostasis and metabolic stress responses. It also negatively regulates the mammalian target of rapamycin (mTOR) to support autophagy and inhibit cell growth when necessary. Sestrin-2 therefore plays an important role in coordinating responses between these pathways ensuring optimal cellular function during periods of environmental stress or nutrient deprivation.

SESN2 is implicated in conditions related to oxidative stress and metabolic dysregulation such as cancer and type 2 diabetes. Its involvement in cancer is connected to its regulatory role on the mTOR pathway often overactive in tumor growth. The protein’s modulation of AMPK-mTOR signaling can impact cancer cell survival and response to treatment. Additionally in diabetes Sestrin-2 plays a part in maintaining insulin sensitivity and glucose metabolism often disrupted in diabetic patients. Its ability to regulate oxidative stress and cellular metabolism draws a link between Sestrin-2 and these complex disorders emphasizing its potential as a therapeutic target.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Functions as an intracellular leucine sensor that negatively regulates the mTORC1 signaling pathway through the GATOR complex (PubMed : 18692468, PubMed : 25263562, PubMed : 25457612, PubMed : 26449471, PubMed : 26586190, PubMed : 26612684, PubMed : 31586034, PubMed : 35114100, PubMed : 35831510, PubMed : 36528027). In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents mTORC1 signaling (PubMed : 18692468, PubMed : 25263562, PubMed : 25457612, PubMed : 26449471, PubMed : 26586190, PubMed : 26612684, PubMed : 31586034, PubMed : 35114100, PubMed : 35831510, PubMed : 36528027). Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed : 26449471, PubMed : 26586190, PubMed : 35114100, PubMed : 35831510, PubMed : 36528027). This stress-inducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses. May negatively regulate protein translation in response to endoplasmic reticulum stress, via mTORC1 (PubMed : 24947615). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed : 23274085). May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress (PubMed : 18692468). Moreover, may prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (PubMed : 26612684). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (PubMed : 15105503). However, this could not be confirmed (PubMed : 19113821).

Sequence similarities

Belongs to the sestrin family.

Post-translational modifications

Phosphorylated by ULK1 at multiple sites.. Ubiquitinated at Lys-175 by RNF167 via 'Lys-63'-linked polyubiquitination in response to leucine deprivation: ubiquitination promotes SESN2-interaction with the GATOR2 complex, leading to inhibit the TORC1 signaling pathway (PubMed:35114100). Deubiquitinated at Lys-175 by STAMBPL1, promoting the TORC1 signaling pathway (PubMed:35114100). Ubiquitinated by RNF186; ubiquitination mediates proteasomal degradation (PubMed:31586034).

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Product protocols

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Target data

Functions as an intracellular leucine sensor that negatively regulates the mTORC1 signaling pathway through the GATOR complex (PubMed : 18692468, PubMed : 25263562, PubMed : 25457612, PubMed : 26449471, PubMed : 26586190, PubMed : 26612684, PubMed : 31586034, PubMed : 35114100, PubMed : 35831510, PubMed : 36528027). In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents mTORC1 signaling (PubMed : 18692468, PubMed : 25263562, PubMed : 25457612, PubMed : 26449471, PubMed : 26586190, PubMed : 26612684, PubMed : 31586034, PubMed : 35114100, PubMed : 35831510, PubMed : 36528027). Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed : 26449471, PubMed : 26586190, PubMed : 35114100, PubMed : 35831510, PubMed : 36528027). This stress-inducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses. May negatively regulate protein translation in response to endoplasmic reticulum stress, via mTORC1 (PubMed : 24947615). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed : 23274085). May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress (PubMed : 18692468). Moreover, may prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (PubMed : 26612684). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (PubMed : 15105503). However, this could not be confirmed (PubMed : 19113821).
See full target information SESN2
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