Recombinant Human SET/TAF-I protein is a Human protein, in the 1 to 290 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A P K R Q S P L P P Q K K K P R P P P A L G P E E T S A S A G L P K K G E K E Q Q E A I E H I D E V Q N E I D R L N E Q A S E E I L K V E Q K Y N K L R Q P F F Q K R S E L I A K I P N F W V T T F V N H P Q V S A L L G E E D E E A L H Y L T R V E V T E F E D I K S G Y R I D F Y F D E N P Y F E N K V L S K E F H L N E S G D P S S K S T E I K W K S G K D L T K R S S Q T Q N K A S R K R Q H E E P E S F F T W F T D H S D A G A D E L G E V I K D D I W P N P L Q Y Y L V P D M D D E E G E G E E D D D D D E E E E G L E D I D E E G D E D E G E E D E D D D E G E E G E E D E G E D D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
Alternative names
Protein SET, HLA-DR-associated protein II, Inhibitor of granzyme A-activated DNase, PHAPII, Phosphatase 2A inhibitor I2PP2A, Template-activating factor I, IGAAD, I-2PP2A, TAF-I, SET
Recommended products
Recombinant Human SET/TAF-I protein is a Human protein, in the 1 to 290 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q01105-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A P K R Q S P L P P Q K K K P R P P P A L G P E E T S A S A G L P K K G E K E Q Q E A I E H I D E V Q N E I D R L N E Q A S E E I L K V E Q K Y N K L R Q P F F Q K R S E L I A K I P N F W V T T F V N H P Q V S A L L G E E D E E A L H Y L T R V E V T E F E D I K S G Y R I D F Y F D E N P Y F E N K V L S K E F H L N E S G D P S S K S T E I K W K S G K D L T K R S S Q T Q N K A S R K R Q H E E P E S F F T W F T D H S D A G A D E L G E V I K D D I W P N P L Q Y Y L V P D M D D E E G E G E E D D D D D E E E E G L E D I D E E G D E D E G E E D E D D D E G E E G E E D E G E D D
- Accession
- Q01105
- Predicted molecular weight
- 35.93 kDa
- Amino acids
- 1 to 290
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab167879 is purified using conventional chromatography techniques.
General info
- Function
Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
- Sequence similarities
Belongs to the nucleosome assembly protein (NAP) family.
- Post-translational modifications
Isoform 2 is phosphorylated on Ser-15 and Ser-24.
- Subcellular localisation
- Nucleus, Nucleoplasm
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as SET.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SET/TAF-I also known as Inhibitor of Protein Phosphatase 2A (I2PP2A) is a multifunctional protein with an approximate mass of 39 kDa. It is ubiquitously expressed in various human tissues including the brain liver and testis. SET/TAF-I plays a role in chromatin remodeling and gene transcription regulation by inhibiting protein phosphatase 2A (PP2A) activity. The protein interacts with nucleosome assembly and in chromatin sliding affecting the overall structure and function of chromosomal material.
- Biological function summary
In the context of cellular processes SET/TAF-I functions as a histone chaperone and is a part of the nucleosome assembly complex. This protein is involved in nucleosome disassembly and assembly during DNA replication and repair and it modulates transcriptional access for various transcription factors. It regulates the acetylation of histones influencing gene expression levels. The SET/TAF-I also participates in apoptosis and autophagy demonstrating its versatility in cellular control mechanisms.
- Pathways
Studies show that SET/TAF-I engages in the MAPK/ERK signaling pathway which is critical for cell cycle progression and proliferation. It is linked with other proteins such as HMGN proteins and histone deacetylases playing a substantial role in chromatin dynamics and genomic stability. Furthermore SET/TAF-I is central to the signaling pathway that maintains normal cellular functions and responds to stress signals like oxidative stress demonstrating its importance in normal cell regulation.
- Associated diseases and disorders
Abnormalities in SET/TAF-I levels link to Alzheimer's disease and acute myeloid leukemia. In Alzheimer's the protein accumulation leads to tau hyperphosphorylation an important feature of neurodegeneration influencing PP2A's role in phosphatase activity. In acute myeloid leukemia SET/TAF-I dysregulation results in altered cell proliferation and survival signaling implicating proteins like BCL-2 and downstream apoptotic regulators. This demonstrates the protein's involvement in both neurological disorders and malignancies highlighting its broader clinical significance.
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1 product image
Western blot - Recombinant Human SET/TAF-I protein (ab167879)
15% SDS-PAGE analysis of ab167879 (3µg).
All lanes: Western blot - Recombinant Human SET/TAF-I protein (ab167879)
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