Recombinant human SET7 protein is a Human Full Length protein, in the 1 to 366 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB, FuncS.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
M D S D D E M V E E A V E G H L D D D G L P H G F C T V T Y S S T D R F E G N F V H G E K N G R G K F F F F D G S T L E G Y Y V D D A L Q G Q G V Y T Y E D G G V L Q G T Y V D G E L N G P A Q E Y D T D G R L I F K G Q Y K D N I R H G V C W I Y Y P D G G S L V G E V N E D G E M T G E K I A Y V Y P D E R T A L Y G K F I D G E M I E G K L A T L M S T E E G R P H F E L M P G N S V Y H F D K S T S S C I S T N A L L P D P Y E S E R V Y V A E S L I S S A G E G L F S K V A V G P N T V M S F Y N G V R I T H Q E V D S R D W A L N G N T L S L D E E T V I D V P E P Y N H V S K Y C A S L G H K A N H S F T P N C I Y D M F V H P R F G P I K C I R T L R A V E A D E E L T V A Y G Y D H S P P G K S G P E A P E W Y Q V E L K A F Q A T Q Q K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3 (PubMed:11779497, PubMed:11850410, PubMed:12540855, PubMed:12588998, PubMed:16141209). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:12540855, PubMed:12588998, PubMed:16141209). Plays a central role in the transcriptional activation of genes such as collagenase or insulin (PubMed:12588998, PubMed:16141209). Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription (PubMed:16141209). Has also methyltransferase activity toward non-histone proteins such as CGAS, p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins (PubMed:15099517, PubMed:15525938, PubMed:16415881, PubMed:35210392). Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II (PubMed:15099517, PubMed:16415881). Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation (PubMed:15525938, PubMed:16415881, PubMed:17108971). Monomethylates 'Lys-491' of CGAS, promoting interaction between SGF29 and CGAS (By similarity).
Alternative names
KIAA1717, KMT7, SET7, SET9, SETD7, Histone-lysine N-methyltransferase SETD7, Histone H3-K4 methyltransferase SETD7, Lysine N-methyltransferase 7, SET domain-containing protein 7, SET7/9, H3-K4-HMTase SETD7
Recommended products
Recombinant human SET7 protein is a Human Full Length protein, in the 1 to 366 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab167960 was determined to be 40 nmol/min/mg as per activity assay protocol.
- Accession
- Q8WTS6-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M D S D D E M V E E A V E G H L D D D G L P H G F C T V T Y S S T D R F E G N F V H G E K N G R G K F F F F D G S T L E G Y Y V D D A L Q G Q G V Y T Y E D G G V L Q G T Y V D G E L N G P A Q E Y D T D G R L I F K G Q Y K D N I R H G V C W I Y Y P D G G S L V G E V N E D G E M T G E K I A Y V Y P D E R T A L Y G K F I D G E M I E G K L A T L M S T E E G R P H F E L M P G N S V Y H F D K S T S S C I S T N A L L P D P Y E S E R V Y V A E S L I S S A G E G L F S K V A V G P N T V M S F Y N G V R I T H Q E V D S R D W A L N G N T L S L D E E T V I D V P E P Y N H V S K Y C A S L G H K A N H S F T P N C I Y D M F V H P R F G P I K C I R T L R A V E A D E E L T V A Y G Y D H S P P G K S G P E A P E W Y Q V E L K A F Q A T Q Q K
- Accession
- Q8WTS6
- Protein length
- Full Length
- Predicted molecular weight
- 75 kDa
- Amino acids
- 1 to 366
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Assessed by densitometry. Affinity purified.
General info
- Function
Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3 (PubMed:11779497, PubMed:11850410, PubMed:12540855, PubMed:12588998, PubMed:16141209). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:12540855, PubMed:12588998, PubMed:16141209). Plays a central role in the transcriptional activation of genes such as collagenase or insulin (PubMed:12588998, PubMed:16141209). Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription (PubMed:16141209). Has also methyltransferase activity toward non-histone proteins such as CGAS, p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins (PubMed:15099517, PubMed:15525938, PubMed:16415881, PubMed:35210392). Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II (PubMed:15099517, PubMed:16415881). Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation (PubMed:15525938, PubMed:16415881, PubMed:17108971). Monomethylates 'Lys-491' of CGAS, promoting interaction between SGF29 and CGAS (By similarity).
- Sequence similarities
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET7 subfamily.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SET7 also known as SETD7 or KMT7 is a lysine methyltransferase that adds methyl groups to lysine residues on target proteins influencing their function and stability. This protein has a molecular mass of approximately 55 kDa. SET7 is expressed in a variety of tissues including the heart liver and pancreas suggesting its versatile role across different biological systems.
- Biological function summary
SET7 contributes to gene regulation by methylating histone H3 at lysine 4 (H3K4) a mark generally associated with transcriptional activation. It also methylates non-histone proteins such as p53 TAF10 and STAT3 affecting their activity and interactions. SET7 operates distinctively and does not need to be part of any complex to fulfill its function effectively in diverse cellular processes.
- Pathways
SET7 modulates transcriptional pathways such as the p53 signaling pathway and the NF-kB pathway. It interacts with the p53 protein affecting cell cycle regulation and the response to DNA damage. The modification of NF-kB by SET7 can influence inflammation and immune responses. Through these pathways SET7 plays a role in maintaining cellular homeostasis and response to stress.
- Associated diseases and disorders
SET7 is involved in conditions like cancer and cardiovascular diseases. Its methylation of p53 links it to tumor suppression activities while its impact on NF-kB relates to inflammatory responses. Dysregulation of SET7 activity can lead to abnormal cell proliferation and inflammation pointing to its potential as a target for therapeutic intervention.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
SDS-PAGE - Recombinant human SET7 protein (ab167960)
SDS Page analysis of ab167960
Functional Studies - Recombinant human SET7 protein (ab167960)
The specific activity of ab167960 was determined to be 40 nmol/min/mg as per activity assay protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com