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AB198126

Recombinant human SET7 protein

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Recombinant human SET7 protein is a Human Fragment protein, in the 52 to 266 aa range, expressed in Baculovirus infected Sf9 cells, with >62%, suitable for SDS-PAGE, FuncS.

View Alternative Names

KIAA1717, KMT7, SET7, SET9, SETD7, Histone-lysine N-methyltransferase SETD7, Histone H3-K4 methyltransferase SETD7, Lysine N-methyltransferase 7, SET domain-containing protein 7, SET7/9, H3-K4-HMTase SETD7

2 Images
Functional Studies - Recombinant human SET7 protein (AB198126)
  • FuncS

Supplier Data

Functional Studies - Recombinant human SET7 protein (AB198126)

Activity of ab198126

SDS-PAGE - Recombinant human SET7 protein (AB198126)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human SET7 protein (AB198126)

SDS-PAGE analysis of ab198126.

Key facts

Purity

>62% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His tag N-Terminus GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

50 μl reaction mix (50 mM TRIS pH8.8, 5 mM MgCl2, 4 mM DTT, 20 μM S-adenosylmethionine, and 0-10 ng SET9) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated K4 residue of histone H3, incubate 1 hr. Then incubate 30 mins with HRP-labeled secondary antibody. Finally, add HRP chemiluminescent substrates and read luminescence.

Accession

Q8WTS6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.49% Glutathione, 0.48% Tris, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MHHHHHHGGGSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFKDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDPENLYFQGGGEFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":266,"aminoAcidStart":52,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q8WTS6","tags":[{"tag":"His","terminus":"N-Terminus"},{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SET7 also known as SETD7 or KMT7 is a lysine methyltransferase that adds methyl groups to lysine residues on target proteins influencing their function and stability. This protein has a molecular mass of approximately 55 kDa. SET7 is expressed in a variety of tissues including the heart liver and pancreas suggesting its versatile role across different biological systems.
Biological function summary

SET7 contributes to gene regulation by methylating histone H3 at lysine 4 (H3K4) a mark generally associated with transcriptional activation. It also methylates non-histone proteins such as p53 TAF10 and STAT3 affecting their activity and interactions. SET7 operates distinctively and does not need to be part of any complex to fulfill its function effectively in diverse cellular processes.

Pathways

SET7 modulates transcriptional pathways such as the p53 signaling pathway and the NF-kB pathway. It interacts with the p53 protein affecting cell cycle regulation and the response to DNA damage. The modification of NF-kB by SET7 can influence inflammation and immune responses. Through these pathways SET7 plays a role in maintaining cellular homeostasis and response to stress.

SET7 is involved in conditions like cancer and cardiovascular diseases. Its methylation of p53 links it to tumor suppression activities while its impact on NF-kB relates to inflammatory responses. Dysregulation of SET7 activity can lead to abnormal cell proliferation and inflammation pointing to its potential as a target for therapeutic intervention.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3 (PubMed : 11779497, PubMed : 11850410, PubMed : 12540855, PubMed : 12588998, PubMed : 16141209). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed : 12540855, PubMed : 12588998, PubMed : 16141209). Plays a central role in the transcriptional activation of genes such as collagenase or insulin (PubMed : 12588998, PubMed : 16141209). Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription (PubMed : 16141209). Has also methyltransferase activity toward non-histone proteins such as CGAS, p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins (PubMed : 15099517, PubMed : 15525938, PubMed : 16415881, PubMed : 35210392). Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II (PubMed : 15099517, PubMed : 16415881). Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation (PubMed : 15525938, PubMed : 16415881, PubMed : 17108971). Monomethylates 'Lys-491' of CGAS, promoting interaction between SGF29 and CGAS (By similarity).

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET7 subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3 (PubMed : 11779497, PubMed : 11850410, PubMed : 12540855, PubMed : 12588998, PubMed : 16141209). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed : 12540855, PubMed : 12588998, PubMed : 16141209). Plays a central role in the transcriptional activation of genes such as collagenase or insulin (PubMed : 12588998, PubMed : 16141209). Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription (PubMed : 16141209). Has also methyltransferase activity toward non-histone proteins such as CGAS, p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins (PubMed : 15099517, PubMed : 15525938, PubMed : 16415881, PubMed : 35210392). Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II (PubMed : 15099517, PubMed : 16415881). Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation (PubMed : 15525938, PubMed : 16415881, PubMed : 17108971). Monomethylates 'Lys-491' of CGAS, promoting interaction between SGF29 and CGAS (By similarity).
See full target information SETD7
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